Thermo Scientific Accela Pump, Hardware Manual

Page: 43 / 50

4
Troubleshooting
Detector-Related Problems
Thermo Scientific Accela RI Detector Hardware Manual 31
Excessive baseline drift Flowcell contaminated Flush the flowcell with cleaning solvents as described
in
“Flushing the Flowcell” on page 25 . Check for
leaks.
Mobile phase contamination Replace the mobile phase with fresh mobile phase
made with high-purity solvents.
Material bleeding from the column Clean or replace the column.
Leaks in system or flowcell Check all of the fittings for leaks. Call a Thermo
Fisher Scientific field service engineer to replace the
flowcell.
Tiny bubble trapped in the flowcell Increase the flow rate until the bubble is removed.
Connect a backpressure device to the flowcell outlet
(and check backpressure rating to avoid rupturing the
flowcell).
Excessive temperature fluctuations Remove the system from drafts. Thermostatically
control the column temperature.
No peaks, or peaks
much smaller than
expected Lamp not on or defective Make sure the lamp is on. Run the detector
diagnostic tests to check the lamp. Call a Thermo
Fisher Scientific field service engineer to replace the
lamp.
Integrator input voltage not
matching output voltage Verify that the integrator is connected to the
appropriate analog output connections on the
detector. Check the attenuation setting on the
integrator.
Insufficient sample reaching the
detector Check the entire system for leaks. Verify the sample
injection volume.
Broad, tailing peaks Excessive rise time Lower the rise time selection.
Poor connection at flowcell inlet Check the end of the inlet tubing for a clean, flat
surface, free of obstructions.
Detector failing to
power up Tripped circuit breaker at power
outlet Reset the circuit breaker.
Blown detector fuse Replace the fuse. See “Replacing the Fuses” on
page 26 .
Incorrect voltage Verify that the line voltage is within the specified
range as listed in
“Specifications” on page 7 .
Power cord not connected Connect the power cord.
Detector not running
upon injection Detector not receiving Start signal Check the connection to the Run contacts on the
back panel.
Table 6. Detector-related problems (Sheet 3 of 4)
Symptom Cause Solution