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Introduction

Functional Description

Thermo Scientific

Accela RI Detector Hardware Manual

5

Figure 5.

Solvent path with purge control on or off

316 stainless tubing

316 stainless tubing (closed when purge control is off)

Teflon™ tubing

Connection by female nut

Connection by flange

NC = Normally closed
NO = Normally open
COM = Valve exit

To liquid waste
receptacle

From column

NO

NC

COM

PURGE On

PURGE Off

Flowcell

Sample
compartment

Reference

compartment

Heat
exchanger

Heat
exchanger

Joint block

3-way solenoid
valve

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Summary of Contents for Accela Pump

Page 1: ...Accela RI Detector Hardware Manual 60257 97002 Revision A February 2010 ...

Page 2: ... All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that ...

Page 3: ...mo Fisher Scientific or one of its authorized representatives Accela RI Detector EMC Directive 2004 108 EC EMC compliance has been evaluated by TÜV Rheinland of North America Inc Low Voltage Safety Compliance Low voltage safety compliance has been evaluated by TUV Rheinland of North America Inc This device complies with Low Voltage Directive 2006 95 EC and harmonized standard EN 61010 1 2001 IEC 6...

Page 4: ...ulations Use of this instrument in a manner not specified by Thermo Fisher Scientific could impair any protection provided by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturing ...

Page 5: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Page 6: ...ieurs compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont di...

Page 7: ...ati si raffreddino prima di effetturare l intervento di manutenzione Fire Use care when operating the system in the presence of flammable gases Feuer Beachten Sie die einschlägigen VorsichtsmaBnahmen wenn Sie das System in Gegenwart von entzündbaren Gasen betreiben Incendie Agir avec précaution lors de l utilisation du système en présence de gaz inflammables Fuego Tenga cuidado al operar el sistem...

Page 8: ...s and proper procedures to dispose waste oil Heat Before servicing the instrument allow any heated components to cool Fire Use care when operating the system in the presence of flammable gases Eye Hazard Eye damage could occur from splattered chemicals or flying particles Wear safety glasses when handling chemicals or servicing the instrument General Hazard A hazard is present that is not included...

Page 9: ... Loosening the Transport Screws 12 Placement and Environmental Requirements 13 Making the Back Panel Connections 14 Connecting to the Data System Computer 15 Connecting the System Interconnect Cable 16 Connecting the Inlet and Outlet Tubing 18 Turning On the Accela RI Detector for the First Time 21 Chapter 3 Routine Maintenance 23 Recommended Routine Maintenance 23 Preventive Maintenance 24 Cleani...

Page 10: ...Contents x Accela RI Detector Hardware Manual Thermo Scientific Appendix A Accessories and Maintenance Parts 35 Accessory Kit 35 Fuses 36 Service Spare Parts 36 Index 37 ...

Page 11: ...m Getting Connected Guide Accela UHPLC Instrument Control by the ChromQuest CDS Versions 4 2 and 5 0 User Guide Safety and Special Notices Make sure you follow the precautionary statements presented in this guide The safety and other special notices appear in boxes Safety and special notices include the following CAUTION Highlights hazards to humans property or the environment Each CAUTION notice ...

Page 12: ...ick Customer Manuals in the left margin of the window Y To suggest changes to documentation or to Help Complete a brief survey about this document by clicking the link below Thank you in advance for your help Send an e mail message to the Technical Publications Editor at techpubs lcms thermofisher com Tip Highlights helpful information that can make a task easier Phone 800 532 4752 Fax 561 688 873...

Page 13: ...tector Hardware Manual 1 1 Introduction The Accela RI Detector Figure 1 is a member of the Accela family of LC instruments Figure 1 Accela RI Detector Contents Functional Description Front and Back Panels Status LEDs Specifications ...

Page 14: ...ety of applications Integrated instrument control from the ChromQuest data system which provides automated temperature control a rise time filter a purge function an autozero function and built in calibration procedures This section contains the following topics Optical System next section Flowcell on page 4 Optical System The optical design of the Accela RI Detector is based on Snell s Law of Ref...

Page 15: ...e image of slit no 2 through the flowcell and onto the photodiode When the refractive indices of the sample and reference solvents in the flowcell differ the image on the photodiode moves horizontally as illustrated in Figure 4 Displacement Reference compartment Light Sample compartment Light beam at the time of ns nr Light beam at the time of ns nr nr ns ns Refractive index of liquid in sample co...

Page 16: ...uring normal operation the reference compartment is shut off from the solvent path You can replace the solvent in the reference compartment by using the purge control feature provided in the data system Figure 5 on page 5 is a diagram of the solvent path through the flowcell depending on how you have set up purge control When the Purge Control is On solvent flows through both the sample and the re...

Page 17: ...ess tubing 316 stainless tubing closed when purge control is off Teflon tubing Connection by female nut Connection by flange NC Normally closed NO Normally open COM Valve exit To liquid waste receptacle From column NO NC COM PURGE On PURGE Off Flowcell Sample compartment Reference compartment Heat exchanger Heat exchanger Joint block 3 way solenoid valve ...

Page 18: ... Jose CA 95134 U S A This device complies with FCC Rules Part 15 Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation DISCONNECT INPUT POWER BEFORE OPENING COVER REFER ALL SERVICE TO QUALIFIED PERSONNEL ONLY CAUTION T3 15A 250V T1 6A 250...

Page 19: ...shed Amber There is no communication with the data system Run Green The detector is ready for a run Flashing green A run is in progress and the detector is sending data to the data system computer Amber The detector is not ready to start a run Flashing amber An error occurred during the run Temp Green The detector has reached a stable temperature Amber The temperature of the detector has not equil...

Page 20: ...n of autozeroing 10 10 9 RIU Offset adjustable range 0 to 500 mV same with Integrator output sensitivity Resolution of offset 10 mV same with Integrator output sensitivity Integrator output 0 to 1V FS Sensitivity 512 10 6 RIU FS Recorder output 0 to 10 mV FS External communication Ethernet Event marker input Contact DC 5V 0 1 A Purge on off Automatic zeroing on off Run Signal output Contact 5V 0 1...

Page 21: ...including how to connect it to the other modules of the Accela LC system Contents Installation Checklist Unpacking and Inspecting Checking the Fuses Loosening the Transport Screws Placement and Environmental Requirements Making the Back Panel Connections Connecting the Inlet and Outlet Tubing Turning On the Accela RI Detector for the First Time ...

Page 22: ...e following A CD containing the software and documentation An accessory kit containing an Ethernet cable with fittings and tubing to connect the detector see Accessory Kit on page 35 If any items are missing contact your Thermo Fisher Scientific representative immediately U Unpack and inspect your instrument See page 10 U Check the fuses See page 11 U Loosen the transport screws See page 12 U Prov...

Page 23: ...tector until you hear a clicking sound 2 Pull the fuse holder out of the power module Figure 10 Figure 10 Fuse holder and fuses CAUTION Do not connect the power cord to your instrument without first verifying that the fuses are correct for the line power at your location Never connect the detector to or operate the detector with an electrical line source with power fluctuations greater than 10 abo...

Page 24: ...bolts located underneath the detector along the right side To use the detector loosen the transport screws with a 5 mm hexagonal wrench provided in the accessory kit Y To loosen the transport screws 1 Place the Accela RI Detector on the laboratory benchtop 2 Locate the 5 mm hexagonal wrench provided in the accessory kit and place it near the detector 3 Turn the detector so that the right side is a...

Page 25: ...sible to the chromatographic column outlet This location minimizes the length of tubing necessary for connection to the flowcell inlet If you are using other Accela modules place the Accela RI Detector on top of the autosampler as shown in Figure 12 on page 14 Ensure that the location meets the following requirements A draft free location away from an open window air conditioner vents or other cir...

Page 26: ...ela LC system Making the Back Panel Connections Use the cables provided in the Accela RI Detector accessory kit to make the back panel connections described in this section This section contains the following topics Connecting to the Data System Computer next section Connecting the System Interconnect Cable on page 16 ...

Page 27: ...sing the supplied CAT5 7 ft long shielded Ethernet cable with ferrite clamp Figure 13 Ethernet connections Note A CAT5 Ethernet cable is the only cable shipped with the Accela RI Detector If you already have an Accela LC system the Ethernet switch and system interconnect cable are already part of your existing system If you just purchased an Accela LC system locate the Ethernet switch and cables i...

Page 28: ...cle located on the back panel of the Accela RI Detector as shown in Figure 13 on page 15 2 To connect an autosampler plug the unlabeled connector to the left 8 pin receptacle located on the back panel of the autosampler 3 To connect a pump plug the connector labeled PUMP to the 8 pin receptacle located on the back panel of the pump DETECTOR DETECTOR DETECTOR PUMP PUMP A S M S To MS detector To aut...

Page 29: ...ENET UNIT ID 910111213141516 GND GND TF 1 TF 2 TF 3 TF 4 5V 150mA GND 1 2 3 4 5 6 7 8 A S READY INJECT OUT INJECT HOLD PUMP READY GRAD START PUMP STOP 5V 150mA GND 1 2 3 4 5 6 7 8 9 0 1 2 3 4 5 6 7 8 9 0 ENET 910111213141516 NC CHASSIS GND INTEGRATOR INTEGRATOR NC CHASSIS GND RECORDER RECORDER 1 2 3 4 5 6 7 8 PURGE GND ZERO READY RUN NC 5V 150mA GND Connector labeled DETECTOR Connector labeled PUM...

Page 30: ...n the system connect the other end of the inlet tubing to the outlet of the LC column Figure 16 on page 19 If the Accela RI Detector is the second detector in the system connect the other end of the inlet tubing to the outlet of the flow cell of the first detector Figure 17 on page 20 4 Connect the 3 32 in OD 0 060 in ID Teflon outlet tubing to the OUT port 5 Connect the other end of the outlet tu...

Page 31: ...tions for a single detector configuration A B C D B D C J2 J3 J4 Out In D C B A Out port connection to waste In port connection from LC column 3 32 in OD 0 060 in ID Teflon outlet tubing Accela pump 0 005 in ID 1 16 in OD stainless steel inlet tubing To waste container Accela autosampler Accela RI Detector Drain manifold Convoluted tubing ...

Page 32: ...tific Figure 17 Inlet and outlet tubing for a dual detector configuration Out In A B C D B D C J2 J3 J4 D C B A Accela RI Detector 0 005 in ID 1 16 in OD stainless steel inlet tubing Accela pump To waste container Accela autosampler Accela PDA Detector LC Column 3 32 in OD 0 060 in ID Teflon outlet tubing ...

Page 33: ...la RI Detector is properly connected to the data system computer 3 Ensure that the data system lists the appropriate configuration settings for the Accela RI Detector 4 Plug the power cord into the power receptacle on the back panel of the detector Figure 7 on page 6 and connect it to the power source 5 Turn the power on by pushing in the power switch on the front panel Figure 6 on page 6 Note By ...

Page 34: ......

Page 35: ... you have any questions about proper maintenance or to arrange for a preventive maintenance program contact your Thermo Fisher Scientific field service engineer Recommended Routine Maintenance Table 4 on 24 lists recommendations for routine maintenance of the Accela RI Detector Use this table as a basis for developing your maintenance program in accordance with your company practices Contents Reco...

Page 36: ... If this does not remove the contamination clean the flowcell with a dilute solution of nitric acid followed by a water rinse Thoroughly flush and clean all peripheral plumbing pumps and so on before connecting them to the flowcell To avoid particulate contamination and fluid path blockage maximize the use of filtration Avoid leaving buffers standing in the flowcell for long periods Plan your use ...

Page 37: ...o the flowcell Y To flush the flowcell 1 Prepare a 20 v v solution of nitric acid in HPLC grade water 2 Connect the LC pump directly to the Accela RI Detector 3 Launch the ChromQuest data system The Instrument window appears 4 Choose Control Instrument Status The Instrument Status window appears 5 Click the Accela RI tab 6 Click the RI Diagnostic tab 7 In the Purge Control area click On 8 Pump the...

Page 38: ...see Table 5 If the detector does not power up you might need to replace the fuses See Chapter 4 Troubleshooting to confirm if the fuses require replacement Y To replace the fuses 1 Turn off the power to the detector and unplug the detector from line power 2 Squeeze the lever located just below the fuse holder cover until you hear a clicking sound and then pull the fuse holder out of the power modu...

Page 39: ...lace them with the new fuses provided in the accessory kit Table 5 on page 26 4 Push the fuse holder back into the back panel of the detector until you hear a clicking sound 5 Plug the power cord into the power receptacle on the back panel of the detector 6 Turn the power on by pushing in the power switch on the front panel Fuse holder Lever Fuse holder Fuses ...

Page 40: ......

Page 41: ...ed Detector Related Problems Table 6 lists detector related problems along with suggestions for corrective action Contents Detector Related Problems Error Messages Table 6 Detector related problems Sheet 1 of 4 Symptom Cause Solution Spikes on baseline Continuous gas bubbles in the flowcell Degas the mobile phase Connect a backpressure device to the flowcell check backpressure rating Immiscible so...

Page 42: ...Fisher Scientific field service engineer to replace the flowcell Insufficient lamp warm up Allow a 30 minute warm up for normal operation and 1 5 hours for maximum sensitivity Lamp aging or defective Call a Thermo Fisher Scientific field service engineer to replace the lamp Ground loop problem between integrator and detector Check for the proper cable connections to the detector output do not grou...

Page 43: ... lamp is on Run the detector diagnostic tests to check the lamp Call a Thermo Fisher Scientific field service engineer to replace the lamp Integrator input voltage not matching output voltage Verify that the integrator is connected to the appropriate analog output connections on the detector Check the attenuation setting on the integrator Insufficient sample reaching the detector Check the entire ...

Page 44: ...the temperature to stabilize If the temperature does not stabilize in 30 minutes call your Thermo Fisher Scientific field service engineer for assistance Table 6 Detector related problems Sheet 4 of 4 Symptom Cause Solution Table 7 Error messages Error message Remedy Initialize Device failed The detector is not connected to the data system Shut down and restart the instruments Direct Control faile...

Page 45: ...h heater circuit Call your Thermo Fisher Scientific service representative for assistance Optical balance movement cannot detect home Call your Thermo Fisher Scientific service representative for assistance RAM error Instrument memory failure Call your Thermo Fisher Scientific service representative for assistance Light intensity too low Purge the detector Flush the flowcell with fresh mobile phas...

Page 46: ......

Page 47: ...sory Kit Fuses Service Spare Parts Table 9 Accela RI Detector accessory kit 60253 62000 Part number Description Quantity 97355 98006 CAT5 Ethernet cable 1 00109 02 00014 LuerLok syringe adapter 10 32 1 16 in 1 2522 0285 PEEK fingertight fitting 10 32 2 00006 02 00010 Fuses T3 15 AL250 5 20mm for 100 115V operation 2 00006 02 00011 Fuses T1 6 AL250 5 20mm for 230V operation 2 3301 0151 Syringe Luer...

Page 48: ...em If you just purchased an Accela LC system locate the Ethernet switch and system interconnect cable in Accela system accessory kit Table 10 Fuses Part number Description Quantity 00006 02 00010 Fuses T3 15AL250V 5 20mm for 100 115 V operation 2 00006 02 00011 Fuses T1 6AL250V 5 20mm for 230 V operation 2 Table 11 Service spare parts Part number Description 00950 01 00250 AD DA PCB 00950 01 00251...

Page 49: ...e iii flowcell bubbles in 29 cleaning 25 description 4 leaking 30 schematic 4 fuse size checking 21 fuses replacing 26 specification 36 G ground loop problems causes of 30 I inlet tubing connecting 18 installation checklist 10 L LEDs 7 O operating humidity 8 operating range 2 operating temperature 7 optical system components 3 description 2 outlet tubing connecting 18 P peak problems 31 power requ...

Page 50: ...cela RI Detector Hardware Manual Thermo Scientific Index T system interconnect cable 16 T temperature fluctuations results of 31 transport screws loosening 12 troubleshooting 29 W WEEE compliance v weight 7 ...

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