2013-06
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
25
4.
Calculations
To correct the OD values due to dirt on the outer surfaces of the quartz
lenses, an additional measurement at a reference wavelength is performed
automatically with each measurement. For Infinite M200 PRO and
Infinite M1000 readers, a reference wavelength of 310 nm is used
and for the Infinite F200 PRO and Infinite F500 readers, a reference
wavelength of 340 nm is used.
4.1
Calculation of Nucleic Acid Concentration
After the absorbance measurements of the nucleic acids have been
performed in the NanoQuant Plate, i-control and Excel automatically
calculate the nucleic acid concentration according to the Lambert-Beer law,
including the reference values.
A = ε *
d
* c
A
Absorbance
ε
Molarity Extinction Coefficient (L mol
-1
cm
-1
)
d
Distance (path length
in cm)
c
Concentration (mol L
-1
)
Calculation of DNA concentration:
DNA purity is automatically performed in the background as described in
the following: Generally, in analytical chemistry the absorbance A is defined
as
A
λ
= log
10
�
𝑰
𝟎
𝑰
�
[OD]
where
I
is the intensity of light at a specified wavelength
that has passed through
a sample (transmitted light intensity) and
I
0
is the intensity of the light before it enters the sample.
Absorbance measurements are often carried out in analytical chemistry,
since the absorbance of a sample is proportional to the thickness of the
sample and the concentration of the absorbing species in the sample.
Absorbance is a logarithmic dimension; its unit is OD (Optical Density).
