6
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
2013-06
1.5
Applications
Nucleic Acid Quantification (Infinite 200 PRO, Infinite M1000,
Infinite F500)
For the quantification procedure in the NanoQuant Plate, a sample volume of
2
μl is sufficient for accurate results. Absorbance of nucleic acid samples is
measured at 260 nm. The optical path length of the NanoQuant Plate is
0.5 mm. To assess the purity of the nucleic acid, an additional measurement at
280 nm is performed to indicate proteins in the sample. For pure nucleic acids,
a 260/280 ratio between 1.8 - 1.9 is acceptable. If this ratio is lower than 1.8 it
may indicate the presence of proteins or other contaminants. If this is the case,
an additional purification step/procedure is recommended.
Labeling Efficiency (Infinite M200 PRO and Infinite M1000 only)
Working with nucleic acids labeled with fluorescent dyes requires samples of
high quality. Besides common nucleic acid quantification and nucleic acid purity
check with 260/280, the labeling efficiency is an important criterion for improved
research results. With the NanoQuant Plate it is possible to measure
absorbance of nucleic acids labeled with Cy3, Cy5, Alexa 555, Alexa 647 and
many other fluorescent dyes.
Note
All measurements on Infinite M1000 involving NanoQuant Applications are
performed using one measurement channel only.
