2013-06
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
3
1.
General Information
1.1
Introduction
Tecan’s NanoQuant Plate is intended as a general laboratory measurement
tool for the quantification of small volumes (2
μl) of nucleic acids in absorbance
mode and the measurement of the labeling efficiency of nucleic acids labeled
with fluorescent dyes. The NanoQuant Plate permits the application and
parallel measurement of 16 different samples in a single measurement
procedure. After the measurement, which is controlled by Tecan’s i-control
software, the calculation of nucleic acid content and purity check using the
260/280 ratio is performed automatically and the results are displayed in an
Excel sheet. A blanking measurement, including an integrated reference
wavelength at the beginning of the measurement procedure, functions
simultaneously as a quality control check for the entire plate and indicates any
pipetting or cleaning mistakes. The plate has been designed to meet the
requirements of research laboratories working with various types of low-volume
samples including fluorophore-labeled nucleotides.
1.2
Contents of the NanoQuant Plate Package
The NanoQuant Plate package for Infinite reader series contains the following
items:
•
NanoQuant Plate
•
Pipetting Aid
•
Safety Certificate
•
This Quick Guide
•
Storage Box
The NanoQuant Plate is available for the following readers:
•
Infinite M200 PRO
•
Infinite F200 PRO
•
Infinite M200 PRO NanoQuant
•
Infinite F200 PRO NanoQuant
•
Infinite M1000
•
Infinite F500