26
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
2013-06
Example
An absorbance value of 1 OD means a transmittance of 10%, and an
absorbance value of 2 OD means a transmittance of 1%, etc. Because
absorbance calculations are based on logarithmic dimensions, calculations
between absorbance sample values and absorbance blank values are
performed by subtraction and not by division. For more information review
the Lambert-Beer law.
4.2
Blanks
Average Blanking
The average absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500
) of all wells
used for blanking is subtracted from the average absorbance value at
260 nm or 280 nm. The relative variation of the wells used for average
blanking must be below 10% in order to be able to start a measurement.
Infinite M200 PRO and Infinite M1000
Abs
blank average
= Abs
260 average
– Abs
310 average
[OD]
Infinite F200 PRO and Infinite F500
Abs
blank average
= Abs
260 average
– Abs
340 average
[OD]
Individual Blanking
The well-specific absorbance value at 310 nm for Infinite M200 PRO and
Infinite M1000 (340 nm for Infinite F200 PRO and Infinite F500
) is
subtracted from the corresponding absorbance value at 260 nm.
Infinite M200 PRO/Infinite M1000
Abs
blank A1
= Abs
260 A1
– Abs
310 A1
[OD]
Abs
blank A2
= Abs
260 A2
– Abs
310 A2
[OD]
Abs
blank B1
= Abs
260 B1
– Abs
310 B1
[OD]
etc.
