8
Quick Guide NanoQuant Plate
TM
No.30035094 Rev No. 1.4
2013-06
4. Select the desired measurement type in the control bar on the left side
of the window by double-clicking or dragging and dropping:
•
Nucleic acid quantification (Infinite 200 PRO, Infinite M1000,
Infinite F500)
•
Labeling Efficiency (Infinite M200 PRO and Infinite M1000
only)
5. The corresponding measurement stripe appears and the NanoQuant
Plate definition file (NanoQuant Plate Tecan 16 Flat Black) is
automatically selected in the Plate field.
6. Select blanking mode. Select the
Individual Blanking
check box for
individual blanking or leave the check box clear for average blanking.
See Individual Blanking and Average Blanking on page 12.
7. Depending on the connected instrument, the wavelengths used for
measurement are selected automatically (make sure that the correct
filters are properly installed and defined on the filter slides of the
Infinite F200 PRO and Infinite F500
instrument).
Instrument
Bandwidth at 260 nm
Bandwidth at 280 nm
Infinite M200 PRO:
5 nm
5 nm
Infinite F200 PRO:
5 nm
3 nm
Infinite M1000:
5 nm
5 nm
Infinite F500:
5 nm
3 nm
8. Select a sample type (e.g. dsDNA, ssDNA, RNA, etc. in the Sample
type drop-down list.
9. In addition, select the
respective dye(s) in the Markers drop
-down lists
for Labeling Efficiency measurements. If the samples are labeled with
only one fluorophore, set the drop-down list of Dye 2 to None.
10. When all settings are correct, click the
Start Blanking
button to
initialize the blanking measurement. The plate transport moves out
and the user is requested to insert the NanoQuant Plate with the
respective blanking buffer.
