Shel lab BACTRON300, Installation And Operation Manual, Page: 70 / 86

70 | P a g e
USER MAINTENANCE (CONTINUED)
C
ALIBRATE THE
T
EMPERATURE DISPLAY
Note: Performing a temperature display calibration requires a temperature reference device.
Please see the Reference Sensor Device entry on page 12 for device requirements.
Temperature calibrations are performed to match an incubator temperature
display to the actual air temperature inside the incubator. The actual air
temperature is supplied by a calibrated reference sensor device. Calibrations
compensate for long-term drifts in the BACTRON microprocessor controller as
well as those caused by the natural material evolution of the sensor probe in the humid and heated
chamber space. Calibrate as often as required by your laboratory or production protocol, or
regulatory compliance schedule.
Each temperature display and its incubator must be calibrated separately in the BACTRON900.
Probes
Reference device sensing probes may be introduced into an incubator
through the door space. In the cabinet style incubators, the wire may be
introduced through the finger hole in either door.
Place the sensor probe head as close as possible to the geometric center of
the incubator. A thermocouple sensor probe sleeve may be taped to the
shelving using non-stick, non-marking tape, as long as the exposed copper
end is 2 inches (5cm) away from the shelf (see Figure 34). An exposed
sensor probe in direct contact with the shelving may experience heat sinking,
which can result in an inaccurate temperature reading.
In a side storage incubator, carefully close the incubator door over the probe
wire, and, if possible, latch the door. Use non-stick, non-marking tape to
secure the wire and to seal any gaps.
Stability
Prior to calibration, an incubator should operate undisturbed at its application temperature set point
for 8 hours in order to stabilize. A common practice is to introduce and place the temperature
sensor probe in the incubator near close of business, allow the unit to operate and stabilize
overnight, and then calibrate the display next morning.
The incubator is considered stabilized when it has operated for 1 hour with no fluctuations of
±0.1°C or greater. Failure to wait for stabilization will result in an inaccurate calibration.
Continued on next page
Figure 34: Probe End 2
inches (5cm) From Shelf
Surface