Shel lab BACTRON300, Installation And Operation Manual, Page: 18 / 86

18 | P a g e
INSTALLATION (CONTINUED)
G
AS
S
UPPLY
R
EQUIREMENTS
Warning
:
Never exceed a 5% hydrogen concentration inside the anaerobic workspace chamber.
Avertissement: La concentration d’hydrogène ne doit pas dépasser
5% dans la chambre anaérobie.
AMG (Anaerobic Mixed Gas) - Required
The BACTRON maintains an anaerobic environment in the workspace chamber in part through a
catalytic oxygen-capturing scrubbing process. The BACTRON requires an anaerobic mixed gas
with 5% hydrogen for this catalysis. Sheldon Manufacturing recommends a source of anaerobic
mixed gas (AMG) with the following ratios:

Hydrogen (H
2
) 5%

Carbon Dioxide (CO
2
) 5%

Nitrogen (N
2
) 90%
Anaerobic Mixed Gas is often sold by gas suppliers under the category of Anaerobic Incubation
Mixtures or Biological Atmospheres . Contact your site safety officer and review your institutional
safety protocols for handling, storing, and using compressed gases. Follow all local ordinances and
national regulations regarding compressed gases in research, clinical, or production environments.
Dual and Single Gas Configuration Options
AMG is also used to cycle the pass box and arm ports. To reduce anaerobic mixed gas
consumption during pass box auto cycle purges, the BACTRON can be connected to a second
compressed gas cylinder containing a cheaper inert gas such as nitrogen (N
2
), for use in the first
two stages of the three-stage auto cycle.

For dual gas configurations, Sheldon Manufacturing recommends one cylinder of AMG gas
at the above ratios along with a cylinder of 100% Nitrogen (N
2
).

The BACTRON will not draw from the second cylinder during manual pass box cycles or
when cycling the arm ports.
Requirements - All Configurations

All gases must be medical grade . Non-medical grade gas supplies may contain
impurities that can contaminate samples and damage BACTRON components. Use of
non-medical grade gases voids the manufacturing defect warranty.

Gas regulators for BACTRON applications must be set at 15 - 20 psi . Please see the
Pressure Unit Conversion table on page 56 in the Operation Section for working with
units other than Pounds per Square Inch.

Always use a dual-stage regulato r for each supply cylinder to ensure precise flow rates.

Use the filtered gas supply tubing kit provided with the AMG regulator.
Figure 7: Gas
Tubing Kit