Shel lab BACTRON300, Installation And Operation Manual, Page: 42 / 86

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OPERATION (CONTINUED)
C
HAMBER
E
NTRY
Perform these steps to enter and work in the workspace chamber without drawing in aerobic
atmosphere. See the
Exiting the Chamber procedure (page 46) for how to withdraw without
compromising the anaerobic atmosphere. You must don the BACTRON sleeve assemblies prior
to entering the chamber.
1. Remove watches, bracelets, large rings, and any sharp objects that might damage the
sleeves or cuffs.
2. Place your hands in the sleeve assemblies so the cuff rings sit snug against bare skin on
the widest part of your forearms.
a. The BACTRON comes with mid-sized, size 8 cuffs. Please see the Parts List on page
78 for other cuff sizes.
Cycling the Sleeves
3. Evacuate air from the sleeve assemblies by depressing the VAC foot pedal (right).
a. Hold your hands approximately 4
– 6 inches (10 cm – 15 cm) away from the arm port
doors, and slightly to either side. This helps prevent the collapsing sleeves from pulling
your hands into the arm port doors.
b. Both sleeves will deflate simultaneously.
c. Continue deflating until the definition of your fingernails is visible through the sleeves.
4. Flush the sleeves with AMG mixture by depressing the Gas foot pedal (left).
a. Stop as soon as you can no longer see the definition of your fingernails through the
sleeve, and the sleeve material does not cling to your forearms.
b.
Do not overfill the sleeves. “Ballooning” the sleeves wastes gas, and risks
compromising the seals around the cuff rings and arm ports.
5. Repeat steps 3 and 4 for three complete iterations of evacuation and AMG replacement.
Entry
1. Loosen both arm port doorknobs by 2 or 3 turns.
2. Rotate the locking bars to roughly 45° diagonals:
3. Slowly push one door into the chamber, then the second.
a. Avoid pushing both doors in the same time. Pushing both doors in simultaneously
creates a significant displacement of pressurized chamber atmosphere.
4. Stow the arm ports doors, one at a time.
a. BACTRON300 and BACTRON900
– place the arm port doors one at a time in the
hanging door shelves on the bottom of the workspace chamber incubator.
b. BACTRON600
– Place the arm port doors one at a time in the arm port door stands
included with the unit.