Shel lab BACTRON300, Installation And Operation Manual, Page: 52 / 86

52 | P a g e
OPERATION (CONTINUED)
Manual Cycle
Users may manually cycle the pass box. This option is provided as a backup for the auto cycle.
1. Both pass box doors must be closed and latched, with the Doors light illuminated on the
main control panel, to safely cycle the pass box.
a. Do not cycle the pass box if the Pass Box Doors light is not illuminated!
2. Depress the Manual switch to the right (VAC).
a. Hold the switch down until the pass box pressure display shows between -17 inHG
and -20 inHg of atmospheric pressure in the pass box.
b. Immediately cease vacuuming if the water in the manometer gauge bubbles or the gas
injection light illuminates frequently, interrupting evacuation of the pass box. There may
be a leak along the inner pass box door. Continuing to vacuum may damage the
BACTRON. Contact Technical Support.
3. After achieving the low atmosphere pressure target in step 2, depress the Manual switch to
the left (GAS) to inject AMG into the pass box.
a. Hold the switch in this position until the pass box pressure display reads
approximately -4inHg.
b. This completes one iteration.
4. Repeat steps 3 and 4 twice more. Three iterations of evacuation and AMG backfill are
required to fully cycle the pass box.
a. On the third and final iteration, hold the Manual switch to the left (GAS) until the
pressure display reads 0.5inHg . This puts the pass box pressure near the workspace
chamber overpressure, allowing the inner pass box door to be opened without difficulty.
b. The pass box interior door can now be opened.
5. The Anaerobic indicator light does not illuminate after the completion of a manual cycle.
6. Do not open the outer pass box door while the inner pass box door is opened !
7. Always close an open pass box door after offloading or loading items.
a. This safeguards against accidental exposure to oxygen from an inattentive user
opening the outer door while the inner door is open.
Note: Failure to cycle the pass box to the standards described above risks introducing aerobic
atmosphere into the workspace chamber.