Shel lab BACTRON300, Installation And Operation Manual, Page: 37 / 86

37 | P a g e
OPERATION (CONTINUED)
E
STABLISH AN
A
NAEROBIC
A
TMOSPHERE
Carry out the following steps to purge aerobic atmosphere from the workspace chamber and
incubators, and establish an anaerobic atmosphere. If the BACTRON has previously been in use,
remove the right arm port sleeve prior to starting the procedure. This procedure requires
approximately 1 to 4 hours to complete.
Continued on next page
Establish an Anaerobic Atmosphere
1. Verify that the BACTRON is connected to an AMG gas source.
a. A dual gas configuration is acceptable. An AMG source with a 5%
hydrogen concentration must be attached to the GAS 1 IN port
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2. Note the reading on the AMG gas regulator.
a. The BACTRON300 requires approximately 200 psi of AMG for
the initial setup purge.
b. The BACTRON600 and BACTRON900 need around 400 psi of
AMG for the initial setup purge.
Note : Sufficient AMG should be on hand to continue operating the BACTRON
after setting up the anaerobic atmosphere.
AMG
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3. Verify all incubator doors are slightly open (0.5 inches / 1cm) to avoid
leaving reservoirs of aerobic atmosphere.
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4. Verify the arm port doors are closed and sealed.
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5. Check that the manometer has been filled to the fill line (upper ring)
with purified water.
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