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Shel lab BACTRON300 Installation And Operation Manual Download Page 3

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BACTRON Anaerobic Workstation 110 - 120 Volts

Installation and Operation Manual

Part number (Manual): 4861700-1

Revision: January 20, 2016

Pictured on Cover: BACTRON900 (BACTRONIV-900)

BACTRON600 (BACTRONIV)

BACTRON300 (BACTRONII)

These units are TÜV CUE listed as anaerobic chambers for professional, industrial, or educational
use where the preparation or testing of materials is done at an ambient air pressure range of 22.14
– 31.3 inHg (75 – 106 kPa) and no flammable, volatile, or combustible materials are being heated.

These units have been tested to the following requirements:

CAN/CSA C22.2 No. 61010-1:2012

CAN/CSA C22.2 No. 61010-2-010:2004 Reaffirmed: 2014-07

UL 61010-1:2012-05

UL 61010A-2-010:2002-03

EN 61010-1:2010

EN 61010-2-010:2014

Supplemented by: UL 61010-2-010:2015

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Summary of Contents for BACTRON300

Page 1: ...BACTRON 110 120 Volts Installation and Operation Manual BACTRON300 BACTRON600 BACTRON900 Previously Designated BACTRONII BACTRONIV BACTRONIV 900...

Page 2: ...2 P a g e This page left blank...

Page 3: ...professional industrial or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa and no flammable volatile or combustible...

Page 4: ...onnect the Foot Pedal Controls 21 Fill the Manometer 22 Installation Cleaning and Disinfection 22 Place Anaerobic Monitoring Strips 23 Install Shelf Spacers 23 Prepare the Incubators 23 Install the Ar...

Page 5: ...Usage 63 Sleeve Maintenance and Usage 63 Replacing Sleeve Assembly Components 64 Cleaning and Disinfecting 66 Maintaining the Acrylic Glass Panels 68 Vacuum Pump Maintenance 68 Electrical Components 6...

Page 6: ...s This can result in injury or death Your BACTRON and its recommended accessories are designed and tested to meet strict safety requirements The workstation is built to connect to a wall power source...

Page 7: ...ance CONTACTING ASSISTANCE If you are unable to resolve a technical issue with the BACTRON please contact Sheldon Technical Support Phone hours for Sheldon Technical Support are 6am 4 30pm Pacific Coa...

Page 8: ...rt any damage to the carrier service that delivered the BACTRON If the carton is not damaged open the carton and remove the contents Carefully check all packaging before discarding Save the shipping c...

Page 9: ...Manometer Side Storage Incubator Pass Box Workspace Chamber Workspace Chamber Incubator Catalyst Cartridge Gas and Vacuum Ports Workspace Incubator Control Panel Side Storage Incubator Pass Box Right...

Page 10: ...ed on back RECEIVING YOUR BACTRON CONTINUED Workspace Chamber Incubator Catalyst Cartridge Gas and Vacuum Ports Pass Box Right Arm Port Control Panel Manometer Left Arm Port Power Outlet Vacuum Pump P...

Page 11: ...tion Figure 6 Pass Box Inner Door and Sliding Shelf The pass box style airlock is provided with an integral sliding shelf to facilitate material transfers Small items may be introduced into the chambe...

Page 12: ...can be introduced into the incubator leaving the device outside Readings taken outside the incubator avoid incubator door openings during verifications and calibrations and eliminate subsequent waits...

Page 13: ...13 P a g e RECEIVING YOUR BACTRON CONTINUED This page left blank...

Page 14: ...resent page 15 Check that a suitable electrical outlet and power is present page 16 Install the BACTRON in a suitable location Review lifting and handling instructions page 17 Make sure the BACTRON is...

Page 15: ...onnections Please page 74 for unit dimensions Caster mounted stands for BACTRONs are available for purchase see page 82 ENVIRONMENTAL DISRUPTION SOURCES When selecting a location to install your BACTR...

Page 16: ...or these units are 15 amps The wall power source must conform to all national and local electrical codes The unit must be positioned so that all end users can quickly unplug the BACTRON in the event o...

Page 17: ...ng and damage Note To prevent damage when moving the BACTRON turn each of the four leveling feet completely clockwise LEVELING The BACTRON must be level and stable for safe operation Each BACTRON ship...

Page 18: ...le Gas Configuration Options AMG is also used to cycle the pass box and arm ports To reduce anaerobic mixed gas consumption during pass box auto cycle purges the BACTRON can be connected to a second c...

Page 19: ...the filtered gas tubing kit to the barb adapter of the T fitting Attach the black tubing of the kit to the AMG regulator Note Failure to connect the T fitting to both the GAS 1 and 2 ports will interf...

Page 20: ...re to connect the vacuum pump included with the BACTRON 1 Place the vacuum pump in a well ventilated location adjacent to the BACTRON a The pump must be ventilated to prevent overheating An overheated...

Page 21: ...bly b Connect the branching lines to the two brass barb adapter fittings on the inner sides of the arm port door assemblies Figure 15 Foot Pedal to Arm Port Assemblies Connect the foot pedal supply li...

Page 22: ...he factory procedure may not meet the standards of your institutional protocols Cleaning and disinfecting the workstation during installation reduces the chance of microbiological contamination Please...

Page 23: ...e anaerobes or if the incubator is being run at high temperatures PREPARE THE INCUBATORS Open all incubator doors to create a 0 5 inch 1cm gap The incubator doors must be placed slightly ajar prior to...

Page 24: ...ion 2 Install each door bottom first inserting the tabs into the slots on the arm ports 3 Tilt the door cover up so that it sits securely in the arm port 4 Turn the locking bar to the horizontal posit...

Page 25: ...25 P a g e INSTALLATION CONTINUED This page left blank...

Page 26: ...ntaires Indicates Temperature Rep re temp rature Indicates the Over Temperature Limit system Indique le syst me de d passement de temperature Indicates AC Power Rep re le courant alternatif Indicates...

Page 27: ...ent in the pass box Indique un environnement ana robie dans le sas Indicates an aerobic environment in the pass box Indique un environnement a robie dans le sas Indicates a injection of gas Indique un...

Page 28: ...28 P a g e GRAPHIC SYMBOLS CONTINUED This page left blank...

Page 29: ...Panel Incubator Controls BACTRON300 The Incubator controls on the main panel control the operations of the workspace incubator BACTRON600 The Incubator controls on the main panel control the operatio...

Page 30: ...heating of the chamber in the event of a failure of the main digital controller For more details please see the Over Temperature Limit System description in the Theory of Operations page 35 The red Ov...

Page 31: ...ates the when the pass box is atmosphere is being evacuated during auto cycles The Vac Light does not turn on when manually cycling the pass box Manual Switch The Manual Gas OFF VAC switch allows the...

Page 32: ...in its temperature calibration mode and then enter a display value correction The indicator light labeled Heating Activated illuminates whenever the temperature control system is heating the incubati...

Page 33: ...ours of usage Additionally the BACTRON is programmed to maintain a mild overpressure in the chamber to prevent the infiltration of external atmosphere including the diffusion of molecular oxygen throu...

Page 34: ...ugh the sleeve assemblies Incubators BACTRON300 and BACTRON900 are each provided with a cabinet style incubator in the workspace chamber The BACTRON600 and BACTRON900 come with two rotating shelves La...

Page 35: ...re Gauge and Check Valve The water filled manometer in the workspace chamber serves as a visual pressure gauge and dynamic venting check valve during instances of excess overpressures The manometer is...

Page 36: ...he back of the BACTRON 2 Insert the power cord plug into the room power source outlet 3 Power the BACTRON by placing the main control panel power switch in the On I position a The power switch and all...

Page 37: ...as configuration is acceptable An AMG source with a 5 hydrogen concentration must be attached to the GAS 1 IN port 2 Note the reading on the AMG gas regulator a The BACTRON300 requires approximately 2...

Page 38: ...s will not successfully purge workstation 10 Open and remove the right arm port door 11 Unwrap and install a fresh or reactivated catalyst cartridge on the left wall inside the workspace chamber a The...

Page 39: ...the arm port knob until the interval decreases b If gas continually pulses into the chamber tighten the right arm port door knob until an injection interval of 1 to 2 seconds is established Continual...

Page 40: ...on for 2 hours whichever happens first tighten and secure the right arm port door Then carry out the Installing the Sleeve Assemblies Chamber Entry and Verifying Anaerobic Atmosphere procedures on the...

Page 41: ...ve over the lip of the arm port door a Place the ring on the large end of the sleeve inside the groove on the arm port b Make sure none of the sleeve material is trapped or pinched between the ring an...

Page 42: ...sleeves will deflate simultaneously c Continue deflating until the definition of your fingernails is visible through the sleeves 4 Flush the sleeves with AMG mixture by depressing the Gas foot pedal l...

Page 43: ...f oxygen The strips turn pink in the presence of oxygen The more color on the strip the more oxygen is present The strips do not lose color after having been exposed to O2 A strip hold its pink colori...

Page 44: ...te a controlled leak that aerobic atmosphere can be expelled through See the Establish an Anaerobic Environment procedure steps on page 39 b Allow an additional 50 psi or 30 minutes worth of AMG to fl...

Page 45: ...ranched supply line is securely connected to the barb adaptors on both arm ports 7 Check that the foot pedal assembly GAS and VAC lines are connected to the GAS OUT and VAC OUT ports on the BACTRON 8...

Page 46: ...r arm port door 4 Withdraw arms from the sleeves one at a time Door Seal Check Carry out the following steps to make sure the arm port doors are sealed 1 Hold the sleeves away from the BACTRON allowin...

Page 47: ...the letters SP to indicate the Set Point is about to be shown b The temperature display will then show the flashing adjustable temperature set point Note The display will automatically exit the adjust...

Page 48: ...ther door Place the sensor probe head as close as possible to the geometric center of the incubator A thermocouple sensor probe sleeve may be taped to the shelving using non stick non marking tape as...

Page 49: ...falls within the acceptable range of your protocol the display is accurately showing the chamber air temperature The Temperature Verification procedure is now complete b See step 2 if a difference fa...

Page 50: ...es in the workspace chamber are generating heat If you can find no sources of external or internal temperature fluctuations contact Tech Support or your distributor for assistance End of Procedure Set...

Page 51: ...outer pass box door while the inner pass box door is opened 4 Always close an open pass box door after offloading or loading items a This safeguards against accidental exposure to oxygen from an inat...

Page 52: ...o the left GAS to inject AMG into the pass box a Hold the switch in this position until the pass box pressure display reads approximately 4inHg b This completes one iteration 4 Repeat steps 3 and 4 tw...

Page 53: ...the containers Syringes should be loosened and sealed containers partly opened if permitted by your laboratory or production protocol The pass box sliding shelf can hold and transport up to 252 plates...

Page 54: ...ladium coated pellets inside the catalyst cartridge should grow warm in the presence of oxygen and hydrogen indicating that the cartridge is ready for use 2 If the cartridge does not heat up when expo...

Page 55: ...rst beaker of carbon media with the second 3 On day 3 reactivate the first beaker of carbon media a Heat at a minimum of 160 C for at least two 2 hours Place the reactivated scrubber in the chamber b...

Page 56: ...anufacturing recommends the use of distilled water in the resistance range of 50K Ohm cm to 1M Ohm cm or a conductivity range of 20 0 uS cm to 1 0 uS cm for cleaning and humidifying applications HUMID...

Page 57: ...57 P a g e OPERATION CONTINUED This page left blank...

Page 58: ...h Record Incubator Temperature Catalyst Changed Condensate Receptacle Drained AMG Cylinder Pressure Reading Monitoring Strip Changed Date 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24...

Page 59: ...level in the manometer While the chamber is operating under its normal overpressure of 0 5 inches the water level should be even with the lower of the two reference rings 10 Clean and disinfect the w...

Page 60: ...ng AMG into the workspace chamber to main overpressure Injections are accompanied by a clicking noise that is the chamber gas solenoid opening then closing Verify Chamber Overpressure Verify that the...

Page 61: ...the acrylic glass panels arm port doors the outer pass box door back panel as well as the side incubator in the BACTRON600 and 900 The manometer exhaust port on the back of the BACTRON will register...

Page 62: ...rittleness dryness or cracks are present b Clean the gaskets with warm water and soap if sticky or dirty Dry and seat securely on the pass box mounting frames 6 Close and latch both pass box doors a V...

Page 63: ...needs to be adjusted Adjusting the chamber pressure switch is a service level procedure Locating leaks See the Locating Leaks entry on page 61 for instructions on using a hydrogen leak detector to pi...

Page 64: ...uff ring 4 Inspect all components for brittleness dryness or cracks Replace as needed Reassemble the sleeve assembly 1 Pull the sleeve cuff on over the cuff ring 2 Roll an O ring onto the cuff ring ov...

Page 65: ...65 P a g e USER MAINTENANCE CONTINUED This page left blank...

Page 66: ...the parts of the equipment or with material contained in it Warning Never clean the unit with alcohol or flammable cleaners Avertissement Ne jamais nettoyer l appareil l alcool ou avec des nettoyants...

Page 67: ...lable disinfectants that are non corrosive non abrasive and suitable for use on stainless steel painted surfaces and acrylic glass Contact your local Site Safety Officer for detailed information on th...

Page 68: ...result in a quick loss of elasticity Disable or redirect laboratory UV lighting away from the BACTRON The BACTRON should not be exposed to direct sunlight Verify that your laboratory or workspace envi...

Page 69: ...ng the acrylic glass panels or metal bulkheads Managing excessive condensation at humidity levels that overwhelm the BACTRON condensate controller depends on either lowering the humidity level in the...

Page 70: ...le in either door Place the sensor probe head as close as possible to the geometric center of the incubator A thermocouple sensor probe sleeve may be taped to the shelving using non stick non marking...

Page 71: ...alls outside of your protocol range advance to step 2 Reference Device 2 A display calibration adjustment must be entered to match the display to the reference device See next step Reference Device 3...

Page 72: ...point with the corrected display value Cooling to Set Point 6 Allow the BACTRON sit for at last one 1 hour undisturbed to stabilize after the incubator has achieved the corrected temperature set point...

Page 73: ...l repeat steps 3 7 up to two more times a Three calibration attempts may be required to successfully calibrate units that are more than 2 C out of calibration Reference Device 9 If the temperature rea...

Page 74: ...units produced in the series We reserve the right to alter technical specifications at all times WEIGHT Model Shipping Weight Unit Weight BACTRON300 610lbs 276kg 328 5lbs 149kg BACTRON600 760lbs 344 7...

Page 75: ...IONS Model Inches W D H Centimeters W D H All Models 12 x 13 5 x 12 30 5 x 34 3 x 30 5 PASS BOX PLATE CAPACITY Model Plates All Models 252 INCUBATOR DIMENSIONS Workspace Chamber Incubator Model Inches...

Page 76: ...17 6 498 BACTRON600 19 538 BACTRON900 17 6 498 Workspace Incubator Volume Model Cubic Feet Liters BACTRON300 1 4 39 6 BACTRON600 BACTRON900 1 4 39 6 Side Storage Incubator Volume Model Cubic Feet Lite...

Page 77: ...odel Range Uniformity Workspace Incubator Uniformity Side Incubator BACTRON300 Ambient 5 C to 70 C 0 5 C 37 C BACTRON600 Ambient 5 C to 70 C 1 C 37 C BACTRON900 Ambient 5 C to 70 C 0 5 37 C 1 C 37 C P...

Page 78: ...d Inlet Type T 12 5Amp 250V 5x20mm 3300520 Arm Port Door Right 9900698 Fuse Vacuum Pump Outlet Type T 10Amp 250V 5x20mm 3300516 Arm Port Door O Ring 6000509 Gas Regulator Kit Anaerobic Mixed Gas 97405...

Page 79: ...g straps 9990738M Sleeve Cuffs Latex Size 9 for sleeve assembly 3600502 Sleeve Assembly Size 9 Large 2 cuffs 2 cuff rings 4 0 rings 2 sleeves 2 self gripping straps 9990738L Sleeve Cuffs Nitrile Size...

Page 80: ...322 4897 extension 3 If you are uncertain that you have the correct Part Number or if you need that specific item please contact Sheldon Technical Support for help at 1 800 322 4897 extension 4 or 503...

Page 81: ...Number 9490578 For 110 120 volt units Acrylic Glass Cleaner 2oz 59 2ml Novus brand acrylic glass cleaner Part Number 1060503 Acrylic Glass Scratch Remover 2oz 59 2ml Helps remove visible scratches an...

Page 82: ...N900 Microscope Adaptors Designed for the Lecia S6 Spotting Stereo Microscope BACTRON300 Part Number 9990535 BACTRON600 and BACTRON900 Part Number 9990511 BACTRON300 Stand A rolling stand with cabinet...

Page 83: ...he appropriate BACTRON microscope adaptor Part Number 9990516 Lukas Fiber Optic Micro Lite Illumination System A fiber optic adjustable brightness halogen light box and guide Provides a stable long la...

Page 84: ...P a g e ACCESSORIES CONTINUED UV Viewing Lamp A handheld UV lamp for use with BACTRON workstations Parts Number 9490507 Zephiran Benzalkonium Chloride Chamber Cleaner 1 Gallon 0 133 Part Number 10605...

Page 85: ...85 P a g e ACCESSORIES CONTINUED...

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