Last updated 10/29/2020
5
Cryo Screening with Tietz TVIPS F416
The TVIPS is a CMOS (complementary metal-oxide-semiconductor) 16 megapixel camera with a 50
frame/sec readout (4k x 4k). It sits below the column of the microscope, below the DE20 camera (thus,
the DE20 must be retracted to be able to use the TVIPS). It is a robust camera that has good SNR (good
contrast) that is used primarily for screening grids. It sits within its own vacuum housing separate from
the EM vacuum and is not retractable and always cooled. Because the temperature rarely changes on the
camera, dark and gain references are taken infrequently (once per month). It is controlled using “EM-
Menu” software, which is found on the “camera” monitor in monitor input “DVI-2”. Suggested mags for
EN/FA/FC = 62,000x; HL = 5000x; SQ = 1700x.
MCL server (on microscope computer) must be on in order to turn on EMMENU4 (software for F416)
(on camera computer). If F416 computer restarted, login as supervisor.
1.
After step 6 of “F20 User Guide”....
2.
Open EMG or NCCAT Leginon_client (depending on your access type) on camera & microscope
computer
a.
TVIPS camera control on monitor input DVI-2
3.
Open Leginon program on leginon PC
a.
In terminal, type betaleginon if using EMG Leginon_client, or nccatleginon if using
NCCAT Leginon_client.
4.
Setup Leginon session settings:
a.
Select “create new session” → “next”
b.
Select holder (Elsa or TF20-cryo-holder_AMI-2)
c.
In session description, type information about sample → “next”
i.
Make sure the start session name with “F20- ”
d.
Select project → “next”
e.
Image directory (use default… “/gpfs/leginon/username”)
f.
Connect to clients= tf20-f416 & tf20-
d394 → “next”
g.
C2 size = refer to the white board → “finish”
5.
Once Leginon has started up, open the “application”
a.
Application → Run → MSI
-T2 (3.3)
i.
Main = tf20leginon
ii.
Camera = tf20-f416
iii.
Scope = tf20-d394
b.
“Run”
6.
Upload your presets
a.
Preset manager → “import presets from another session” (* icon)
i.
TEM = tecnai; Digital Camera = Tietz F416 (if you do not see a camera
available, restart both clients on EM and camera computers)
b.
“Find” presets from past 20 days; select desired preset (usually from the most recent user)
c.
Highlight all the presets (GR, SQ, HL, FA, FC, EN) and “import” → “done”
i.
Suggested mags for EN/FA/FC = 62,000x; HL = 5,000x; SQ = 1,700x
Summary of Contents for TF20
Page 9: ...Last updated 10 29 2020 8 ...