Last updated 10/29/2020
27
a.
EN preset gains
i.
Prepare beam for target dose = 30 - 60 electrons/Å
2
(SS 5, beam diameter ~ 2X
bigger than second circle on flu screen)
ii.
– Check en presets, and in the Correction node settings, choose the camera of
interest, and set the appropriate camera configuration, binning, and exposure
time.
iii.
Dark reference
1.
Close column valves and flu screen down
2.
In “correction” node → “settings” →
see LEFT image below
→ “dark”
and “both channels”
3.
Acquire (you should see black image)
Summary of Contents for TF20
Page 9: ...Last updated 10 29 2020 8 ...