SEMC TF20, User Manual, Page: 23 / 30

Last updated 10/29/2020
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Cryo Holder Insertion/Retraction Guide for F20
***Cryo holder insertions on the F20 may only be performed by SEMC staff. NO EXCEPTIONS. ***
Cryo Holder insertion:
1. Before insertion confirm that IGP1 = 6, column valves closed,
holder is reset, objective aperture out, flu screen down, airlock
default pump time = 120 sec, turbo on and ready (button will be
orange while warming up and yellow when ready).
2. Set holder alpha value at -60 degrees. Goniometer will rotate.
3.
“Pre-pump airlock.” Takes 4 seconds.
4. Position styrofoam box below goniometer to catch LN
2
.
5. Remove holder from the transfer station and rotate the holder so
that the holder top is at the 3 o’clock position. Immediately insert
holder going straight into the goniometer with holder at 3 o’clock
position until holder can go no further in.
a. You should see the airlock pump time initiate in the
Vacuum Overview window
6.
Select ‘ST Cryo Holder’ as specimen holder