Last updated 10/29/2020
18
d.
Transfer cryo grid to the tip of the holder.
e.
Secure the grid with the clip ring. Place clip ring
side of the tool onto the grid until you feel a click.
While applying a small amount of vertical pressure
on the end of the clip ring tool, slowly turn the tool
COUNTER- clockwise 1 - 2 turns until the tool
moves freely. Pull up to remove the clip ring tool.
f.
Close the shield of the cryo holder.
4.
Insert Elsa holder into the microscope
a.
Insertion is the same as the 626 holder.
5.
Collect data
6.
Retract Elsa holder from the microscope
a.
Retraction is the same as the 626 holder.
7.
Warm up the holder
a.
Dump out all excess liquid nitrogen from the dewar of the holder.
b.
Insert holder into the dry pumping station and turn it on.
c.
Once “MDP status” and “System Status” turn green, open V2 and then V1.
d.
Attach the holder to the Elsa temperature controller.
e.
Run the “Warm up” cycle. This takes ~2 hours.
8.
On Fridays, pump down the dewar (open V3 and V4).
If an overnight collection was done and you want to use the holder the next day, on the next day:
●
If you have time…
retract the holder and run warm up cycle (takes 2 hours); after warm up you
can then cool down the holder and use as normal
●
If you don’t have time…
cool down the holder in the scope (with the high-resolution cord
attached so you can measure the temperature of the tip); after the tip reaches ~ -170C, you can
retract the holder into a cold transfer station and switch to the grid of your choice (you will
discard the current grid since it has warmed up in the column and is unusable now)
Summary of Contents for TF20
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