Last updated 10/29/2020
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iv.
Bright reference
1.
Open column valves and flu screen up
2.
In “correction” node → “bright” and “both channels”
3.
Acquire (you should see image of the camera sensor)
v.
Confirm that correction is done
1.
Under “correction” and “both channels”
2.
Acquire (you should see a flat fielded image)
vi.
When done with en preset gains, make sure to apply that same brightness/beam
diameter to the en preset in Preset_Manager
b.
FA/FC/HL/SQ/GR preset gains (SS 5, beam diameter ~ 2X bigger than second circle on
flu screen; target dose = 20 - 30 electrons/Å
2
).
i.
– Check FA preset (especially camera size and binning), and in the Correction
node settings, choose the camera of interest, and set the appropriate camera
configuration, binning, and exposure time.
ii.
Repeat step 13a using settings from
RIGHT image below
.
a.
Summary of Contents for TF20
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