Last updated 10/29/2020
20
b.
Care should be taken as LN
2
is added to the filling cup as
excess LN
2
will pour out of the back of the transfer station.
3.
Transfer cryo grid to the holder.
a.
The gridbox will sit in a small pool of LN
2
on the 9’o clock
side of the tip. See image →
b.
All tools are in the drawer labeled “CT3500 only”. There are
two tools that are used to handle the grid and clip ring: 1)
clipping tool and 2) flat head tool. See image below.
c.
The clipping tool is used for loading/unloading
clip ring and the flathead tool is used to remove
the clip ring if it has been over-tightened. Rotate
counterclockwise to remove clip ring off the tip
of the holder and clockwise to secure the clip
ring in place.
d.
When unscrewing the lid off the gridbox, you
will need to use a pair of forceps to hold the box
down (station was originally designed for
square boxes).
4.
Transfer cryo holder into the TEM.
a.
Same insertion mechanism as 626 holder
b.
The CT3500 does NOT have a pin that secures
it to the goniometer plate (you need to rotate it
to approximately zero degrees by eye)
c.
After insertion, warm up the cryo transfer
station (it’s not recommended to keep it cold
since it gets very frosty)
d.
Open column valves when IGP1 < 20 (will not
go < 10).
5.
After screening, retract holder from the TEM.
Summary of Contents for TF20
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