Section 10
Integra 3™ Troubleshooting
64
Research Instruments Ltd
10
Problem
Possible cause
Solution
The specimen
falls off the
holding pipette/
unable to get
pressure to
inject
Tip of the
micropipette
holder has not
been tightened
properly
Tighten the tip if necessary
Micropipette tip
could be blocked
Replace the micropipette
There may be
an air leak in
the injection/
aspiration system
Check the connection of the syringe tubing
at each end. If loose, cut 10mm from the
end of the tubing and reconnect. Check the
tubing for damage along length and replace
if necessary. Replace the O-ring in the
micropipette holder tip (see Figure 11-1 on
page 66) if worn out. Replace the O-ring in
the SAS air syringe (see Figure 11-3 on
page 68)
Poor control
from SAS Syringe
Check for:
- Air leaks as above
- Blocked pipette
Poor control
from SOS Syringe
Check for:
- Air bubbles
- Blocked pipette
- Using correct medium and oil eg a sterile
filtered paraffin oil
- Oil leaks (treat as for air leaks)
Unable to
aspirate/inject
Has the specimen
dried up?
Heated Stage warmth can dry up an
insufficiently protected specimen. A generous
layer of mineral oil over the specimen will
protect it from drying out.
Are the media you
are using within
their shelf life?
Some media and PVP deteriorate if stored
past their shelf life or if they have not been
kept refrigerated during storage or transit.
Summary of Contents for INTEGRA 3
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