Research Instruments INTEGRA 3, User Manual, Page: 39 / 76

Section 7
Integra 3™ Temperature Control
35
Research Instruments Ltd
7
Probe Positioned in Droplet Centred
over Objective/Hole in Heated Plate
Probe Positioned in
Droplet over Heated Plate
Where the Thermosafe Air Heating System is fitted, it is recommended
that temperature measurements are made above the centre of the
objective
and
over the heated plate (see Figure 7-1 on page 35).
For optimum temperature control across the hole in the heated plate,
adjust the setpoint temperature of the Thermosafe Air Heating System.
To control the area directly above the heated plate, adjust the heated
plate setpoint temperature. As a starting point, use the same setpoint
temperature for both heating systems.
In all cases, it is recommended that the objective normally used for
manipulation is in position when measuring specimen temperature and
adjusting setpoint temperatures.
Figure 7-1
Temperature Calibration
Perform calibration only if the displayed temperature is different to the
actual temperature of the heated plate surface or the air expelled from the
air heater nozzle. The process of calibration allows the user to manually
adjust the temperature so that the displayed temperatures match the
temperature of the heated plate or air expelled from the air heater nozzle.
We recommend using a thermometer calibrated to 37°C fitted with a
small thermocouple probe, such as the RI IVF Thermometer. Place the
probe of the thermometer in the centre of the water drop and allow the
temperature reading to stabilise. Adjust the setpoint temperature until
the desired temperature in the dish is reached, allowing 20 minutes (or
as long as required) in between each setpoint change to allow the Petri
dish temperatures to stabilise.