Research Instruments INTEGRA 3, User Manual, Page: 22 / 76

Section 5
Integra 3™ Basic Operation
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Research Instruments Ltd
5
Heated Glass Inserts
Place the Petri dish in the centre of the insert, avoiding the frame of the
glass insert (figure 5-3). Short working distance objectives might touch
the insert before the specimen is brought into focus, depending on the
thickness of the glass and the dimensions of the Petri dish. Dishes with
a diameter larger than 60mm may need positioning by hand if the area
covered by specimen preparations exceeds 40mm in diameter.
Heated Stage Plate
The moving stage plate is heated, allowing a dish to be positioned away
from the centre of the stage should this be required.
Heated Metal Inserts
Place the Petri dish such that the area of interest is over the hole in the
centre of the insert. The effective range of movement is limited by the
diameter of this hole (normally 25mm). Reposition the dish by hand to
view specimens that are outside the area of the hole. It may also be
necessary to reposition the dish if specimens are close to the edge of
the hole or if short working distance objectives are being used, as the
objective might contact the insert.
Metal heated inserts are secured in position using magnets, the insert
can be lifted out by pulling upwards. When lowering the insert into
position ensure that the insert is rotated until the magnets re-attach the
insert securely.
The RI Droplet Organisation Guide (Part Number 6-30-600) can be used
to position droplets within commonly used Petri dishes in order to ensure
that all droplets are accessible by the pipettes.
Exercise caution when reaching for stage XY control that the
Fine Control Lever is not knocked, especially when switching
to the Integra 3 after using other stages that have the stage
XY control in a different position.