26
User sample
PF209 element
Permanent fixed slide
Coverslip
Cell culture dish
Coverslip
Plain wafer
Mirror
Semiconductor wafer
Metallic disc
General metallic samples
Metallic disc
6.2 Objective selection
Go to the parameters submenu and click New Objective set
In the following pop-up window, select an option from the list that most closely correlates with the
objective, focusing mechanism and sample you are using. Each option contains a number of pre-set
values, which give a good starting point for configuring each objective. ‘Fixed slide’ options are used
for biological samples containing a glass layer in front of the sample e.g. a coverslip, and ‘materials’
options are used for materials samples e.g. a semiconductor wafer. Refer to the examples below.
User Objective/Focusing Mechanism /Sample Suggested Objective Option
10x/Stepper motor/fixed slide
10x 0.17 apo 0.40 fixed slide
10x/Stepper motor/wafer
10x M achro 0.25 materials
20x/Stepper motor/fixed slide
20x 0.17 apo 0.75 fixed slide
20x/Stepper motor/cell culture dish
20x 0.17 apo 0.75 fixed slide
100x/Piezo nosepiece/wafer
100x M achro 0.90 materials - OP400
10x/Piezo stage/semiconductor wafer
10x M achro 0.25 materials - NZ100
10x/Piezo stage/96-well plate
10x 0.17 apo 0.40 fixed slide - NZ100
Following selection of an objective option, there is an opportunity to rename the objectives with their
actual specifications or with a name that will be recognisable to the user. Changing the name will not
change any of the pre-set values.
6.2.1 Focusing on the sample
Prior Scientific recommends using a 5x, 10x or 20x objective when setting up an objective for the first
time, although any objective can be used. Once steps 6.3-6.7 have been understood, those steps will
be used to set-up all objectives in your imaging system.