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usually be achieved by increasing or decreasing the laser power. See section 4.3.3 for full
details.
Click the Auto Set Centre button, and click ‘Yes’ in the following pop-up box. This will set the
centre of the PureFocus850 sensor, which is used to positively verify whether the sample is in
focus (Fig. 8).
The blue line, which indicates the pinhole centre, will move to the middle of the peak. Half of
the total signal will be on the left side of the line, and other half will be on the right.
4.3.2 Sensor centre setup for line mode systems
In the PureFocus850 GUI, go to the offset submenu and click ‘Go to Factory Home’. The offset
lens will move. Go to the laser submenu and increase the value to 100. Click set.
Go to the mode submenu and ensure line mode is selected.
Set the PureFocus controller to MAN FOC mode.
