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Section 5: Focusing System Setup
If the focusing system is not already connected to the PureFocus850 controller, close the
PureFocus850 GUI and switch off the controller. Plug the focusing system into the 15-pin connector.
Piezo nanopositioning systems require the PF404 BNC to 15-pin D-connector cable. Switch the
controller back on, and restart the GUI. The parameters set during optical alignment do not need to
be recalculated if they were saved to the controller as mentioned in section 4.5.
WARNING: this step is essential for operation of the PureFocus850. If the focus direction is set
incorrectly this can create a positive feedback loop. This can cause a system crash which may
damage your objective or sample. Please read sections 5.1 and 5.2 carefully.
