14
INSTALLATION
Connecting a CO
2
gas cylinder
WARNING
When connecting a gas cylinder to the incubator,
confirm the gas type
.
Confirm that the connections
are secure and that no gas will escape
.
Be sure to use the specified pressure
. Using an incorrect
gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping
gas.
Install the incubator in a location with adequate ventilation.
If adequate ventilation cannot be
provided, then install an alarm system using CO
2
and O
2
densitometers.
1.
Use a liquefied CO
2
gas cylinder (at least 99.5 % pure). The siphon (dip tube) type cannot be used.
2.
Install a gas regulator rated at 25 MPa(G) (250 kgf/cm
2
(G), 3600 psi(G)) for the primary pressure, and
0.25 MPa(G) (2.5 kgf/cm
2
(G), 36 psi(G)) for the secondary pressure on the CO
2
gas cylinder.
3.
Using the gas tube that is provided, connect the gas regulator to the connecting port A/B for CO
2
gas
pipe (located at the lower left-hand side on the rear panel of the incubator).
Note:
If CO
2
is supplied to multiple CO
2
incubators from a single gas cylinder, a CO
2
solid will be formed in the
gas regulator. The gas regulator safety valve will operate, and there may an explosive sound.
When the MCO-21GC is not mounted
Using the gas tube that is provided, connect the gas regulator for the CO
2
gas cylinder to connecting port
A for CO
2
gas pipe on the incubator. After connecting the gas tube, make sure that no gas is leaking.
When the MCO-21GC is mounted
Connect a pair of CO
2
gas cylinders with gas regulators independently. The gas supply line will be
switched automatically. Connect the main cylinder to connecting port A for CO
2
gas pipe and the reserve
cylinder to connecting port B for CO
2
gas pipe. After connecting the cylinders, make sure that no gas is
leaking.
For details on installing the optional gas auto changer (MCO-21GC), refer to the MCO-21GC
installation guide. For details on using the MCO-21GC, refer to page 41.
4.
Set the CO
2
gas on the secondary side to 0.03 MPa(G) (0.3 kgf/cm
2
(G), 4.4 psi(G)) for gas injection. As
the pressure increases, the CO
2
gas density control range will increase. Excessive pressure may cause
gas supply lines inside the incubator to come loose, which may result in gas poisoning or oxygen
deprivation due to the escaping of gas. If gas lines come loose, the incubator must be repaired.
The gas lines connected to the incubator will degrade over time. If any deterioration or abnormalities
are found during inspection, replace the lines immediately.
