PerkinElmer QuantaSmart 1694267, Reference Manual

Page: 38 / 218

CHAPTER 2
28 PerkinElmer Life and Analytical Sciences
How To Run an Alpha Beta Standards Assay
not available on the 2800TR;
optional on the 2900TR and 3100TR series
The following tasks are required to run the Alpha Beta Standards Assay. Two
standards are required, a pure beta emitter and a pure alpha emitter.
1. Choose the Alpha Beta Standards selection from the Libraries menu.
2. Click the Add button and enter the name for a new Alpha Beta Standard Set.
3. Choose Automatic for the Discriminator Type if your pure alpha and pure beta
standards have an activity of at least 50,000 CPM each. If the activity in either
standard is less than 50,000 CPM, choose Manual as the Discriminator Type
by clicking on it.
4. The remaining fields are for information only. They are either default values or
values computed by the instrument.
5. Choose File-New and select Alpha Beta Standards as the assay choice from
the drop down menu.
OR
Open an existing assay and edit or review if necessary.
6. Click the Name button on the Count Conditions Tab to choose from the list of
Alpha Beta Standard names in the Alpha Beta Standards Library. Define the
other available parameters as desired.
Note:High Sensitivity or Low Level Count mode are not available
when counting Alpha Beta Standards, but will be available when
counting samples in an Alpha Beta assay
7. When the assay definition is complete, name and save the assay.
8. Associate (link) the assay parameters with an available protocol flag in the
protocol tree. Place the numbered protocol clip on a cassette.
9. Place the pure beta emitter standard in cassette position 1 and the pure alpha
emitter standard in cassette position 2.
10. Load the cassette onto the instrument and click the green start button at the
top of the main window.
11. After counting is complete, the misclassification (or spillover) curve and the
optimum discriminator value will be stored for the Standard Set. Review the
curve and discriminator setting, if desired, from the Alpha Beta Standards
Library.