IFU_EN_GEN-HT Version 1.01
July2020
51
Slope
The data after slope correction
Normalised
The data after normalisation
Limited
The data after limiting (value set in the Target parameter screen)
Ratio
The ratio (dF/F) of adjacent points (after step removal). This plot is
smoothed with an averaging filter.
Derivative
The gradient of the data (generated with a differentiating filter)
2nd Derivative
The gradient of the derivative (a second application of the same
filter)
Ratio4
An alternative ratio ((F-1-F1)/F0²) that gives an earlier indication of
amplification.
Amplification phases use the steps Raw, Spike, Step, Slope, Normalised, Ratio,
Derivative, Ratio4.
Anneal / melt phases use the steps Raw, Spike, Step, Derivative, 2nd Derivative.
Range X/Y to
Specify the ‘window’ to examine. If the limits are the same, it is treated as a threshold.
Units vary according to phase and plot selected. The meaning can vary according to
type. Limits are inclusive.
Relative to
Adjusts the range ‘window’ relative to the value found by another region.
BE AWARE:
Compatibility of units is not checked – it is possible to make nonsensical
selections (e.g. by making a time range (in seconds) on an amplification plot relative to
an anneal temperature in °C).
Result X/Y
Displays the X and/or Y value found on the results table, provided the region is
detected and used in the result.
Result column
Specifies which column(s) of the results table to put the X/Y value in (default 1). If both
X & Y are selected the X value is in the column specified and Y is in the following
column.
Graph
Show the point found on the graphs, provided the region is detected and used in the
result.
Use as reference
Advanced feature. Allows the point found to be referenced by another target, provided
the region is detected and required by the result.
Additional parameters will be displayed depending on the feature type selected. These are explained
further later.
Regions (other than Threshold type) are independent unless they explicitly use relative ranges; in which
case the region is undefined until the region(s) it is dependent on is/are fully determined. Be careful not
to create circular references as all regions will remain undefined.