IFU_EN_GEN-HT Version 1.01
July2020
49
Excessive step removal will cause flat areas and shifted amplification.
The step removal threshold is target-dependant and is affected by noise:signal ratio of
the assay.
Slope
Slope correction adjusts for drift by examining the initial fluorescence and removing
the slope from subsequent fluorescence data after a given time period (
Time
). Slope
correction occurs after spike and step removal.
Normalisation
Normalisation removes the average initial fluorescence from all fluorescence data after
a given time period (
S/N time
). Slope correction and normalisation both use the same
time.
Control Scope
When the target type is ‘Pos Control’ or ‘Neg Control’, this indicates which group of
wells the control is related to.
None
The control is stand-alone and does not contribute to the result of other
targets
Well
The control only applies to other targets in the same well
Pair
Applies to targets in the same pair (1-2, 3-4, 5-6, 7-8)
Half
Applies to targets in the same half-strip (1-4, 5-8)
Strip
Applies to all targets in the same strip (1-8).
Running controls in a different strip is of dubious validity, so is not directly supported.
Smoothing
This setting adjusts the amount of smoothing applied to anneal derivative plots. If this
value is set to 0, the default smoothing settings in the instrument is used. If this is set
to a value between 1 and 8, the instrument uses an alternative smoothing function
which increases the amount of smoothing as the number is increased.
CONTROL TARGETS
Control targets allow easy configuration of two control types (positive & negative).
A control has a scope which indicates which other targets / wells can be affected. Control scope is
relative to the part of the strip that the sample well is in.
The state of the control is determined by the final result call.
For each well, controls within scope are collected and tested with the required / prohibited options.
Positive and negative controls are treated identically – just collected separately. It is therefore possible
to reverse the meaning or use two positive controls that are handled differently.
Advanced users can create additional controls using references.
