14
This section describes the operating procedures of the reflected light brightfield/darkfield observations which are the
basis of observation methods. The polarization observation and the differential interference contrast observation, etc. are
described in “4 Various observation methods" on page 30.
3
Select the observation light path.
4
Place a sample on the stage.
5
Engage the 10X objective in the light path.
6
Bring the sample into focus.
Stage plate
P.19
X-axis/Y-axis knobs
P.19
Revolving nosepiece
P.20
Coarse/Fine focusing knobs P.21
Brightness control knob
P.22
Interpupillary distance adjustment scale P.23
Diopter adjustment ring
P.23
Revolving nosepiece
P.20
Coarse/Fine focusing knobs P.21
Aperture diaphragm lever
P.26
Field diaphragm lever
P.27
Filter slider insertion slot
P.28
Brightness control knob
P.22
Main switch
P.16
11
Adjust the aperture diaphragm and
the field diaphragm.
(Fully open the aperture diaphragm during darkfield observation.)
12
Insert the required filters.
13
Adjust the brightness.
7
Adjust the brightness.
10
Engage the objective with the magnification
to be used in the light path and
bring the sample into focus.
Observation
Remove the analyzer, polarizer or
filter sliders from the light path.
BF/DF selection lever
P.17
2
Select the brightfield (BF) or
darkfield (DF) observation.
3-1 Reflected light brightfield/darkfield observation procedures
Light path selection knob on trinocular tube
or Observation/SP selection knob on the
side port
1
Set the main switch to (ON).
Eyepiece/camera light path selection
knob
P.18
Operation part
Page
8
Adjust the interpupillary distance.
9
Adjust the diopter
3 3
Basic operations of the microscope
(Reflected light brightfield/darkfield observations)