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4-7 Centering of the centering phase contrast sider IX2-SL (option)
When performing the phase contrast observation using the PHL phase
contrast ring of the centering phase contrast slider IX2-SL or the PH1
phase contrast ring IX2-SLPH1, the centering of the phase contrast ring
must be performed. (The centering of the PH2 phase contrast ring IX2-
SLPH2 is not necessary.)
· The centering of the pre-centered phase contrast slider CKX3-
SLP is not necessary.
· With the phase contrast observation, the aperture iris diaphragm
must be opened.
Engage the phase contrast objective in the light path and bring the
specimen into focus.
Loosen the eyepiece clamping screw a with the small flat blade
screwdriver and remove the eyepiece.
Engage the centering telescope U-CT30-2 in the light path.
Slide the centering phase contrast slider IX2-SL and engage the phase
contrast ring suitable for the objective in the light path.
For procedures to attach the phase contrast ring to the
centering phase contrast slider, see page 40.
Rotate the knurled portion of the centering telescope to focus on the
phase contrast ring slit image b .
Insert the centering knobs d to the centering screw holes (2 positions)
of the centering phase contrast slider. Tighten or loosen them to adjust
so that the phase contrast ring slit image b overlaps with the phase
plate image c of the objective.
Adjust the centering by each objective magnification.
Remove the centering telescope.
Engage the eyepiece in the light path, and tighten the eyepiece
clamping screw a using the small flat blade screwdriver to secure
the eyepiece.
TIP
1
2
3
4
TIP
5
6
TIP
7
8
a
b
c
5
6
d
4-8 Operating procedures of PLN2X and CKX3-SLPAS (option)
Combining the AS ring for 2X CKX3-SLPAS with the objective PLN2X
enables to acquire a high contrast image in a wide field of view.
Slide the pre-centered phase contrast slider CKX3-SLP a and
engage the AS ring for 2X CKX3-SLPAS b attached to the slider in
the light path.
For procedures to attach the AS ring for 2X CKX3-SLPAS b
to the pre-centered phase contrast slider CKX3-SLP a , see
page 40.
Rotate the revolving nosepiece to engage the objective PLN2X in the
light path.
1
TIP
2
a
b
