12
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic
observation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and
IR DIC observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 24.
(Controls Used)
(Page)
Tip for microscope operation
In patch-clamp testing, switch the microscope controls cautiously and gently so that the patch
electrodes do not slip off.
Set the main switch to “
I
” (ON).
@Main switch
²Lamp ON-OFF switch
Select the transmitted light illumination and
adjust its brightness.
³Transmitted/reflected light switch
(P. 14)
|
Light intensity control buttons
(P. 14)
Disengage the filter from the light
path.
5
Filter turret
(P. 15)
Select the binocular light path.
…Light path selector knob
(P. 20)
Place the specimen on the stage.
7
X-axis and Y-axis knobs
(P. 17)
Engage a low-magnification objective in the
light path.
8
Swing lever
(P. 18)
Bring the specimen in focus.
9
Objective up/down buttons, focus
adjustment knob/dial
(P. 16)
Adjust the brightness.
|Light intensity control buttons
(P. 14)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
a
Binocular tube
(P. 19)
b
Diopter adjustment ring
(P. 19)
c
Condenser height adjustment knob
(P. 21)
d
Condenser centering knobs
(P. 21)
e
Field iris diaphragm ring
(P. 21)
Engage the objective to be used in the light
path and bring the specimen in focus.
‡Swing lever
(P. 18)
9
Objective up/down buttons, focus
adjustment knob/dial
Adjust the aperture iris and field iris diaphragms.
(P. 16)
f
Aperture iris diaphragm lever
(P. 22)
e
Field iris diaphragm ring
(P. 21)
Engage the required filters.
5
Filter turret
(P. 15)
Adjust the brightness.
|Light intensity control buttons
(P. 14)
Start observation.
