Olympus BX61WI Instructions Manual Download Page 4

Page: 4 / 56

5 OTHER OBSERVATION METHODS

5-1 Differential Interference Contrast Observation

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24-28

Attaching the Analyzer

Attaching the Polarizer

Attaching the DIC Prisms (for Revolving Nosepiece)

Attaching the DIC Prisms (for Condenser)

Adjusting the Polarizer Position

Observation Method

5-2 Reflected Light Fluorescence Observation

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5-3 Infrared Light (IR)/Differential Interference Contrast (DIC) Observation

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29-32

Introduction

Attaching the IR Modules

DIC Observation Using IR

5-4 Macro Reflected Light Fluorescence Observation

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33-35

Introduction

Attaching the Modules

Filter Characteristics of Fluorescence Mirror Units

Fabricating Optional Mirror Units

6 TROUBLESHOOTING GUIDE

7 SPECIFICATIONS

8 OPTICAL CHARACTERISTICS

36-38

39-40

42-49

ASSEMBLY

— See this section for the replacement of the light bulb. —

24-35

Summary of Contents for BX61WI

Page 1: ...ized Upright Microscope Model BX61WI To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the micro scope Retain this instruction manual in an easily accessible place near the work desk for future reference ...

Page 2: ......

Page 3: ...tives 1 Replacing the Focus Adjustment Knob 2 F C Button 3 Objective Up Down Buttons 4 Objective Escape Return Button 5 Using the Frost Filter Insertion Removal Lever 1 3 Correct assembly and adjustments are critical for the microscope to exhibit its full performance If you are going to assemble the microscope yourself please read Chapter 9 ASSEMBLY pages 42 to 49 carefully For the modules provide...

Page 4: ...ence Observation 29 5 3 Infrared Light IR Differential Interference Contrast DIC Observation 29 32 1 Introduction 2 Attaching the IR Modules 3 DIC Observation Using IR 5 4 Macro Reflected Light Fluorescence Observation 33 35 1 Introduction 2 Attaching the Modules 3 Filter Characteristics of Fluorescence Mirror Units 4 Fabricating Optional Mirror Units 6 TROUBLESHOOTING GUIDE 7 SPECIFICATIONS 8 OPT...

Page 5: ...n error in the focusing block and the motorized focusing opera tion will be suspended Recovery procedure If there is no error in motorized operation the caught object can be removed by turning the focusing knob If there is an error in motorized operation the focusing knob becomes inoperable Disassemble the relevant modules to remove the caught object Replace the relevant modules afterwards Turn of...

Page 6: ...r warrant the electrical safety performance of the equipment Never insert metallic objects into the air vents of the microscope frame as this could result in electrical shock personal injury and equipment damage Safety Symbols The following symbols are found on the microscope Study the meaning of the symbols and always use the equipment in the safest possible manner Symbol Explanation Indicates th...

Page 7: ...d of view may be obscured slightly This is due to the reduction of the light intensity in the field of view due to the narrow aperture and is not due to a defective optical adjustment of the microscope This phenomenon does not affect the photomicrography or TV camera light path 2 Maintenance and Storage 1 Clean all glass components by wiping gently with gauze To remove fingerprints or oil smudges ...

Page 8: ...be U TR30 2 FN 22 U TR30IR FN 22 U ETR3 FN 22 Revolving Nosepiece WI SRE3 WI SNPXLU2 U SLRE WI SSNP with revolving arm Revolving Arm WI NPA Cross Stage IX SVL2 or bridge stage WI XYS Fixed Stage Adapter WI FSH Condenser WI UCD WI DICD WI OBCD U UCD8 U SC3 U AAC U AC2 Focus Adjustment Knob Unit U FH Focus Adjustment Knob Interface U IFFH Microscope Frame BX61WIF Power Supply Unit for 100 W Mercury ...

Page 9: ... plate Y axis knob Page 17 X axis knob Page 17 Centering knobs Storage position Light intensity control buttons Page 14 Light path selector knob Page 20 Reflected light mercury lamp housing See the separately provided instruction manual Allen screwdriver Storage position Lamp voltage indicator Page 14 Mercury lamp power supply unit See the separately provided instruction manual Transmitted reflect...

Page 10: ...nt Frost filterinsertion removal lever Page 16 Light Preset switch Page 14 Lamp ON OFF switch Accepts a 32PO or 32POIR polarizer Indication label attaching frame Filter positions Accepts 32 mm filters with thickness of no more than 6 mm Polarizer clamping knob Page 24 Polarizer rotating dials Page 28 Slider insertion removal stopper Page 15 Filter frame reinforcing ring Page 46 Filter slider Page ...

Page 11: ...sepiece XLU WI SNPXLU2 DIC mount hole Page 25 Swinging Revolving Nosepiece WI SRE3 Swing lever Revolving nosepiece mount dovetail Revolving nosepiece clamping screw Grounding screw hole M3 Microscope frame mount dovetail XLFluor objective mount screw M 34 mm pitch 1 mm UIS objective mount screw RMS Adapter WI RMSAD XLU objective mount screw M 25 mm pitch 0 75 mm UIS objective mount screw Drop prev...

Page 12: ...denser accepts one large or small DIC prism When selecting the brightfield BF light path using the WI UCD leave one DIC prism large mount position empty U LDPW10H U LDPW20H Quarter wave plate rotation ring Page 28 Optical element index mount Page 26 Quarter wave plate clamping knob DIC prism replacement cover Quarter wave plate clamping knob Quarter wave plate rotation ring Page 28 DIC prism Large...

Page 13: ...in the DIC prism position of the WI SNPXLU2 or WI SRE3 Applicable condensers WI DICTHRA2 WI UCD W DICD WI DICT2 U UCD8 Positioning pin Condensers and Applicable Objective Magnifications Condenser Applicable Objective Magnification WI UCD WI DICD WI OBCD 5X or more U UCD8 U SC3 2X or more U AAC 10X or more U AC2 5X or more Oblique iris insertion removal knob Page 22 ...

Page 14: ...ch may affect the potential measurement data during patch clamping Halogen Lamp Power Supply TH4 See the separately provided instruction manual for details Hand Switch TH4 HS Focus Adjustment Knob Interface U IFFH Microscope FRAME connector U FH connector BX UCB connector ...

Page 15: ...ective escape return button Page 16 Lamp ON OFF Connector Objective up button Page 16 Objective escape shelter Focus adjustment knob Objective down button Page 16 F C F C button Page 16 Focusing motor ON OFF Transmitted reflected light switch button Page 14 Option Abbreviations Symbols Function Note Fine Coarse switching Lamp ON OFF Set cancel photo voltage Focus adjustment knob FRAME U FH Z focus...

Page 16: ...ath 5Filter turret P 15 Select the binocular light path Light path selector knob P 20 Place the specimen on the stage 7X axis and Y axis knobs P 17 Engage a low magnification objective in the light path 8Swing lever P 18 Bring the specimen in focus 9Objective up down buttons focus adjustment knob dial P 16 Adjust the brightness Light intensity control buttons P 14 Adjust the interpupillary distanc...

Page 17: ...wever the function of control is not available when the U FH is in use 6 3 9 1 Make a photocopy of the observation procedure pages and post it near your microscope b a 8 7 cdf 5 e 4 9 3 9 2 Control Box BX UCB The U ZPCB T2 Z board can be mounted here See page 44 for the setup and installation of the Z board 9 9 9 ...

Page 18: ...et Switch Fig 4 The Light Preset switch sets the light intensity voltage to a voltage suitable for color photography the factory default is 9 V regardless of the current setting of the light intensity control buttons Achieved by engaging the LBD filter Setting the Desired Brightness Figs 3 4 1 Press the Light Preset switch 5 The PHOTO indicator LED 6 lights up 2 Press one of the light intensity co...

Page 19: ...ew using the provided Allen screwdriver 4 2 Focusing Block The same effect as the focus adjustment knob on the microscope frame can also be obtained using the U FH focus adjustment knob unit However when the microscope is used stand alone while the cable to the U FH is connected the focus adjustment is available only from the focusing knob on the U FH Objective down button Focus Adjustment Knob Un...

Page 20: ...bjective down button 2 to lower the objective The stroke is 25 mm 4 Objective Escape Return Button Fig 9 When replacing the specimen press the Objective Escape Return button 1 The objective will rise by 5 mm in 1 sec Pressing the button again returns the objective to the original height Even after escaping care is still required during objectives switching for the objective may still interfere wit...

Page 21: ...n in Fig 13 The screw hole may sometimes be stuck by paint etc In such a case screw in the M4 screw a few times to expose the metallic thread inside the screw hole and improve the contact before attaching the grounding wire firmly 4 Adjusting the X Axis Y Axis Knob Rotation Tension Fig 14 The rotation tension of the X axis and Y axis knobs can be adjusted inde pendently 1 Loosen the 2 set screws 1...

Page 22: ...holder See page 49 for details 4 4 Revolving Nosepiece If the petri dish in use is filled with liquid it may splash when the objective is switched As such liquids are sometimes toxic be sure to move the revolving nosepiece away from the petri dish before switching the objective Even after the revolving nosepiece has been moved re focusing is easy by making use of the objective escape return button...

Page 23: ...to focus on the specimen Fig 19 Using Finder Eyepieces 1 Looking through the right eyepiece with your right eye turn the top of the eyepiece 2 until a clearly defined double crosslines can be seen in the field of view Figs 19 20 2 Looking through the right eyepiece adjust the focusing using the objec tive up down buttons to bring the specimen and double crosslines into simultaneous focus 3 Looking...

Page 24: ...e mounting frame so that the surface with the model indication faces downward Re attach the micrometer mounting frame in the original position 5 Selecting the Light Path of Trinocular Tube Fig 23 Slide the light path selector knob 1 to select the desired light path Trinocular Tube Light Path Selector Position Pushed In U TR30 2 Binocular 100 Binocular 20 TV photo 80 TV photo 100 U ETR3 Binocular 1...

Page 25: ... condenser holder to move the iris diaphragm image to the center of the field of view Fig 24 Fig A Fig B 7 Gradually open the field iris diaphragm The condenser is properly centered if the iris image is centered and inscribed in the field of view Fig B Fig C During actual use open the field diaphragm slightly until its image cir cumscribes the field of view Fig A Fig B Fig C Field Iris Diaphragm T...

Page 26: ...mination This is possible with objectives from 5X to 100X When the XLUMPlanFI20XW objective is used oblique illumination cannot provide a satisfactory effect due to the high NA 0 95 of the objective The effect of oblique illumination assumes that the field iris image is focused correctly Before proceeding to the following pull out the ob lique iris insertion removal knob 1 and bring the field iris...

Page 27: ...the same refractive index The electrically insulated area and immersion depth of the objective 1 are shown by the range of 2 Do not immerse the entire objective for this will cause malfunction After every immersed use be sure to clean the front lens with neutral detergent Water Immersion Cap for XL Objectives XL CAP In photometering with a film potential sensitive fluorochrome the water sur face f...

Page 28: ...zer deteriorates after it has been subjected to light for a lobe period about 500 hours of continuous use Replace the polarizer when it has been used for a long period Drop the polarizer into the filter insertion position with a push ring 1 or 2 and clamp with the push ring It is recommended to insert the polarizer in insertion position 1 This is because the polarizer rotation clamping knob 3 come...

Page 29: ...taching the DIC Prisms for Condenser Figs 35 to 38 DIC prisms can be inserted in three types of condensers including the WI UCD WI DICD and U UCD8 Do not use the U UCDTP530 one wave plate for the U UCD8 but use the exclusive WI TP137 quarter wave plate Shearing Amount Small High resolution Medium Middle contrast Condenser WI UCD WI DICD U UCD8 with WI TP137 DIC prism for revolving nosepiece WI DIC...

Page 30: ...rism by aligning its positioning pin 5 with the positioning groove 6 in the hole of the turret 4 Fig 36 Be careful not to touch the prism inside the frame A Rotate the turret 4 by 90 clockwise Fig 37 and tighten the two DIC prism clamping screws 3 uniformly using the dedicated knob provided with the condenser Figs 36 37 Do not tighten the screws too much or the prism frame may be deformed B Rotate...

Page 31: ...tion manual provided with the U UCD8 5 Adjusting the Polarizer Position Fig 39 This adjustment is possible without removing the DIC prism for revolving nosepiece However it is not possible if a DIC prism for condenser is engaged in the light path Remove or disengage the DIC prism for con denser as described below WI DICD Remove the DIC prism WI UCD Rotate the turret to engage a position without DI...

Page 32: ...ck interfer ence stripe seen at the center of the eyepiece sleeve s field of view then tighten the clamping knob 2 Ignore the short interference stripes in the surroundings in this adjustment Since this adjustment renders the field of view dark observation cannot be started unless the observation method described in the next item is employed Now the adjustment is complete Attach the eyepiece and o...

Page 33: ... for observing deep areas of a thick brain slice or optic nerve specimen 1 Introduction 1 Since the IR wavelength used is 775 or 900 nm the TV camera in use should be sensitive in the wavelength used Example C2741 79 CCD camera mfd by Hamamatsu Photonics The IR light is harmful to your eyes Avoid visual observation and use the TV monitor whenever possible Should visual observation be used mount th...

Page 34: ...to lens One of PE2X PE2 5X and PE3 3X 1X TV adapter U PMTV1X Mount adapter One of 5 models TV camera IR specification model The mount adapter should be one of 1 U CMAD3 mount adapter 2 U BMAD bayonet mount adapter 3 U IMAD Ikegami camera mount adapter 4 U SMAD Sony camera mount adapter 5 combination of U FMT F T mount U TMAD T mount adapter Note When the contrast is enhanced rather excessively by ...

Page 35: ...LH10IR lamp housing 1 by loosen ing the 3 clamping screws 2 with an Allen wrench width across flats of 2 5 mm 2 While positioning the 45SCF filter 3 so that the arrow on its frame points in the opposite direction of the lamp housing insert the filter in the lamp housing and clamp by tightening the ring spring 4 provided with the filter 3 Attach the collector lens to the original position IR Filter...

Page 36: ...ntroller the diaphragm should be left open here so that the system can manifest full performance IMPORTANT 2 Then engage the IR filter 32BP775 or 32IR900 in the light path by pushing in the filter slider 3 While observing the monitor perform DIC observation using IR a Turn the condenser turret other than the WI DICD to select the DIC prism matching the objective to be used b Engage the objective t...

Page 37: ...so use the U SC3 or U UCD8 swinging condenser If other condenser is combined it will not be possible to illuminate the entire field of view 3 During low magnification fluorescence observation objective switching or stage movement be careful so that the UIS objective does not interfere with the specimen or culture container 4 The low magnification fluorescence objectives have been designed to manif...

Page 38: ...luor4X 340 low magnification fluorescence objective 3 into the position on the shallower side of the U SLRE Sliding Revolving Nosepiece U SLRE 1 Raise the revolving nosepiece mount fully 2 Loosen the revolving nosepiece mount screw 4 on the microscope frame using the Allen screwdriver provided with it 3 Align the mount dovetail 5 of the sliding revolving nosepiece with the revolving nosepiece moun...

Page 39: ...rror Units An optional mirror unit can be fabricated by attaching the custom order barrier filter excitation filter and dichroic mirror to the U MF XL Dimension Conditions of Optical Components of Mirror Unit Barrier filter 32 0 1 0 2 mm max thickness 4 mm Excitation filter 25 0 1 0 2 mm max thickness 6 mm Dichroic mirror See figure on the right When replacing the dichroic mirror take special care...

Page 40: ...set to position Set the light path selector knob to po sition or 20 c Field of view is obscured or not evenly illuminated The light path selector knob is in an in termediate position Set the light path selector knob to a click position according to the pur pose 20 The revolving nosepiece is not in a click position Set it in a click position 18 The condenser is installed incorrectly Re install it 4...

Page 41: ...Secure it by pushing in the sliding dovetail all the way until the stopper 45 The objective is engaged incorrectly in the light path Make sure that revolving nosepiece clicks into place correctly 18 The objective is placed incorrectly may be loose in the revolving nosepiece position Insert the objective all the way into the revolving nosepiece position until it is stopped The stage center plate is...

Page 42: ...ce to match the other so that both sides are of the same type You are not accustomed to parallel opti cal axis When looking into eyepieces do not stare at image from the beginning but see the overall field of view It is some times recommended to turn your eyes away from eyepieces look far off and look into eyepieces again 4 Stage a Stage travel in the horizontal X axis direction stops in the middl...

Page 43: ...m fine adjustment sensitivity of 1 µm Resolution 0 01 µm Maximum stage speed 3 mm sec Stroke per rotation 0 1 mm fine 1 mm coarse Full stroke range 25 mm 4 Revolving nosepiece Model WI SRE3 Swinging Revolving Nosepiece U SLRE Sliding Revolving Nosepiece WI SNPXLU2 Single Position Revolving Nosepiece Attachable modules DIC prisms mountable DIC prisms mountable 5 Observation tube Model U TR30 2 Wide...

Page 44: ...F to 104 F Maximum relative humidity 80 for temperatures up to 31 C 88 F decreasing linearly through 70 at 34 C 93 F 60 at 37 C 99 F to 50 relative humidity at 40 C 104 F Supply voltage fluctuations 10 Pollution degree 2 in accordance with IEC60664 Installation overvoltage category II in accordance with IEC60664 ...

Page 45: ... 4 4 Water immersion impossible UMPlanFI W Water Immersion Universal Plan Semi Apochromat FN 26 5 10XW 0 30 3 30 1 10 100X 20 2 2 20XW 0 50 3 30 0 67 200X 6 1 1 1 LUMPlan W LUMPlan W IR 2 Long WD Water Immersion Universal Plan Semi Apochromat IR 2 For infrared light FN 26 5 40XW 0 80 3 30 0 42 400X 2 0 0 55 40XW IR 2 0 80 3 40 0 42 400X 2 0 0 55 60XW 0 90 2 00 0 37 600X 1 3 0 37 60XW IR 2 0 90 2 0...

Page 46: ...minator The filter turret and cross stage are to be clamped respectively using the special tools provided with them Note For the detailed assembly procedures of the reflected light fluorescence system the BX UCB control box and the TH4 power supply unit refer to their instruction manuals Eyepieces WHN10X FN 22 35WHN10X FN 22 Trinocular tube U TR30 2 FN 22 U ETR3 FN 22 Revolving arm Revolving nosep...

Page 47: ...ing nosepiece clamping screw is not used Turret Reflected light fluorescence illuminator BX RFAA BX URA2 BX RFA Light shielding tube Revolving arm WI NPA Sliding revolving nosepiece U SLRE Revolving nosepiece clamping screw Swinging revolving nosepiece WI SRE3 Clamping screws XLU single position revolving nosepiece WI SNPXLU2 Objective XLFluor2X 340 XLFluor4X 340 RMS adapter WI RMSAD Objective XLU...

Page 48: ...tions Change the setup of the DIP switches to enable the use of the Z board with the BX61WI Changing the On Board DIP Switch Setting Fig 47 Set all other switches than those listed below to the OFF positions S2 Set No 2 and No 3 to ON S3 Set No 2 No 4 and No 5 to ON If the setting is not correct the objective may lower and hit the speci men during initialization Mounting the Z Board Fig 48 Set the...

Page 49: ...ng nosepiece attach the DIC prisms before at taching the revolving nosepiece see page 25 1 Loosen the revolving nosepiece clamping screw 1 using an Allen screw driver and slide in the revolving nosepiece 2 along the mount dovetail 2 Push the revolving nosepiece all the way in and tighten the clamping screw WI SRE3 Only When the microscope has been assembled the confocality adjustment and centering...

Page 50: ...just focusing using the objective on the front side then move the target region in the specimen on the center of the field 2 Switch the objective to that on the front side 3 Insert the centering knobs into the threaded holes 6 and turn them to move the target in the objective on the center After completing centering store the centering knobs in the accommo dation position on the front side of the ...

Page 51: ...r onto the condenser if required The waterproof cover is applicable only to the WI UCD WI DICD and WI OBCD condensers 1 Fit the hole of the waterproof cover 1 in the extremity of the condenser and clamp with the clamping band In DIC observation the condenser has to be removed and attached during adjustments Therefore in this case do not attach the clamping band but just fit the hole of the waterpr...

Page 52: ...ever gently back to the original position to clamp the bulb To prevent reduced bulb life or cracking do not touch the bulb with bare hands If fingerprints are accidentally left on the bulb wipe the bulb with a soft cloth 5 Fit the lamp housing from up and tighten the clamping screw 1 by applying downward pressure Fig 56 Caution for Bulb Replacement During or Right after Use The bulb lamp housing a...

Page 53: ... mounting screw holes 4 and 6 and clamp the cross stage by tightening the hex socket screws with the Allen wrench provided with the microscope frame When the waterproof cover is used attach hex socket screws to the screw holes 4 and 6 by passing through the waterproof cover Lowering the Stage Height When no condenser is used the stage height can be lowered by 50 mm by loosening the 2 holder clampi...

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