Chapter 3 Operation
3.7 Aperture Diaphragm Operation
64
3.7
Aperture Diaphragm Operation
Figure 3-15 Aperture diaphragm open/close lever
70~80
100
Adjust the size of the aperture diaphragm image
to 70-80% the size of the objective pupil plane.
Figure 3-16 Aperture diaphragm adjustment
The aperture diaphragm adjusts the numerical
aperture of the illumination system.
By adjusting the aperture diaphragm, you can adjust
the resolution, brightness, contrast, and focal depth
of the microscope image. Closing the aperture
diaphragm will reduce the resolution and brightness,
and increase the contrast and focal depth. These
properties are interrelated, and cannot be adjusted
independently. Adjust the aperture diaphragm
according to the specimen and purpose.
Aperture diaphragm adjustment is particularly
important for bright-field microscopy, DIC
microscopy, and photomicroscopy. Typically,
adjusting the aperture diaphragm to 70-80% of the
numerical aperture of the objective will result in an
appropriate contrast and a favorable image.
Adjust the aperture diaphragm while looking at the
actual diaphragm image. Move the aperture
diaphragm open/close lever to the left to reduce the
aperture size, or to the right to increase the aperture
size. Adjust the aperture diaphragm so that the size
of the diaphragm image is at 70-80% of the size of
the objective pupil plane.
Adjustment with Bertrand lens on eyepiece tube
B
Figure 3-17 Adjustment with Bertrand lens
When using TI-TD Eyepiece Tube B or TI-TERG
Ergonomic Eyepiece Tube, use the Bertrand lens in
the eyepiece tube for adjustment.
Move the Bertrand lens into the optical path by
moving the Bertrand lens in/out lever at the lower
front of the eyepiece tube to position “B.” Rotate the
Bertrand lens focusing knob on the right to adjust the
focus. This will allow you to view the objective pupil
plane (a bright circle) and the aperture diaphragm
image.
Adjustment with centering telescope
Figure 3-18 Adjustment with centering telescope
When using TI-TS Eyepiece Tube B, use the
centering telescope for adjustment.
Remove one of the eyepieces. Using the adapter,
attach a centering telescope instead. Rotate the
eyepiece of the centering telescope to adjust the
focus. This will allow you to view the objective pupil
plane (a bright circle) and the aperture diaphragm
image.
Aperture
diaphragm
open/close lever
Objective pupil
plane
Aperture diaphragm
image
Bertrand lens
focusing knob
Set to position “B”
Flange
Eyepiece
Summary of Contents for Eclipse Ti-E
Page 1: ...Inverted Microscope Instructions M450 E 07 10 NF 1 ...
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