Nikon Eclipse Ti-E Instructions Manual Download Page 33

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Chapter 2 Microscopy

2.2 Bright-Field (BF) Microscopy

Reset convenient functions.

Coarse

Fine

ExFine

Epi Shutter

FL Block

Refocus

Escape

PFS

OFFSET

OUT

DICHR

OIC MI

RROR

L80

EYE

MEMORY

DISPLAY

Z-RESET

1.5X

BRIGHTNES

R100

L100

FOCUS

PFS

1. Release the condenser refocusing clamp on

the dia pillar illuminator by rotating it
counterclockwise.

2. Open the shutter at the eyepiece by pushing

in the shutter operation lever on the right
side of the eyepiece tube.

3. Move the Bertrand lens out of the optical path

by moving the Bertrand lens operation lever
on the front of the eyepiece tube to position
“O”.

4. Rotate the intermediate magnification

selector dial on the front of the microscope
to the “1x” side.

Turn on the dia illuminator.

Coarse
Fine
ExFine

Obj.

OFF

6V30W

MAX.

12V1

L80

EYE

DISPLAY

MEMORY

PFS

RECALL

Z-RESET

BRIGHTNESS

R100

L100

FOCUS

1. Set the EXTERNAL switch on the back of the

power supply to “ON”.

2. Turn on the power supply by pressing the “I”

side of the POWER switch on the power
supply.

3. Turn on the Ti-E microscope by pressing the

“ON” side of the POWER switch on the back
of the microscope.

The Ti-E microscope is turned on, and the
current status is displayed on the status display
panel.

4 Turn on the lamp by pressing the dia

illumination lamp ON/OFF switch on the left
side of the microscope.

The display content of the status display panel
can be switched between the following three
screens by pressing the DISPLAY switch. Switch
the screen as necessary to display the
information required for the operation.

Display content of the status display panel

Z :

_ _ _ _

0 . 0 0 0 u m

E 1 0 0

C o a r s e

• Upper:

Z-axis position

• Lower:

Optical path | Focus knob resolution

_ _ _ _ _ _ _ _ _ _ _ _

1 0 x / 0 . 2 5

E 1 0 0

C o a r s e

_ _

P F S : O u t

• Upper:

Objective data

• Lower:

Optical path | Focus knob resolution | PFS

_ _ _

1 0 x

Z :

_ _ _ _

0 . 0 0 0 u m

E 1 0 0

C o a r s e

_ _

P F S : O u t

• Upper:

Magnification | Z-axis position

• Lower:

Optical path | Focus knob resolution | PFS

Additional display patterns will become available when

using motorized option devices. For details of the display
content, refer to the instruction manual provided with Hub
Controller A.

1-1

1-2

1-3

1-4

2-2

2-1

2-4

2-3

Summary of Contents for Eclipse Ti-E

Page 1: ...Inverted Microscope Instructions M450 E 07 10 NF 1 ...

Page 2: ......

Page 3: ...been made to ensure the accuracy of this manual errors or inconsistencies may remain If you note any points that are unclear or incorrect please contact your nearest Nikon representative Some of the equipment described in this manual may not be included in the set you have purchased If you intend to use any other equipment with this product read the manual for that equipment too When using TI HUBC...

Page 4: ...e following symbols refer to the instruction manual and read the relevant instructions Symbol Meaning CAUTION HOT This symbol can be found on the top of the dia pillar illuminator and on the 12V 100W lamphouse and cautions the following The lamp and the lamphouse will be extremely hot while and immediately after using the lamp To avoid the risk of burns do not touch the lamp and the lamphouse whil...

Page 5: ...TI ND6 PFS Perfect Focus Unit Safety labels are affixed to the bottom of the TI ND6 PFS Perfect Focus Unit CLASS 1 LED PRODUCT IEC EN60825 1 2001 CAUTION CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTRLY WITH OPTICAL INSTRUMENTS 5 9 3 9 0 1 JAPAN TI ND6 PFS PFS Motorized Nosepiece bottom view 1 Caution label CAUTION CLASS 1M INVISIBLE LED RADIATION WHEN OPEN DO NOT VIEW DIRECTRLY WI...

Page 6: ...rld Under normal use you will not need to pay particular attention to the supplied power voltage TI PS100W Power Supply TI PS100W Power Supply can be used with 100 to 240 VAC at 50 60 Hz and can be used with most wall outlets in the world Under normal use you will not need to pay particular attention to the supplied power voltage TE PS30W Power Supply A or TE PSE30 Power Supply A The input voltage...

Page 7: ...is turned on Follow the cautions below to prevent burns and fire To avoid burns do not touch the lamp and the lamphouse while the lamp is on or for approximately thirty minutes after it has been turned off To avoid the risk of fire do not place fabric paper or highly flammable volatile materials i e gasoline petroleum benzine paint thinner and alcohol near the lamphouse while the lamp is on or for...

Page 8: ...ed mainly for microscopic observations and micromanipulations of specimens such as living cells and tissues in a Petri dish Note the following when handling the specimens Before handling a specimen check whether it is hazardous Wear rubber gloves when handling hazardous i e potentially infectious specimens Be careful not to spill the specimen If a specimen is spilt onto the microscope decontaminat...

Page 9: ...ak electromagnetic waves Do not install the product near precision electronic devices to avoid affecting their performance If signal reception by a TV or radio is affected move the TV or radio set away from the product 4 Cautions on moving the product When carrying the product hold the product firmly by the bottom front recess and the bottom rear When moving the product do not hold by the focusing...

Page 10: ...void placing the product in direct sunlight or immediately under the room lights The image quality is degraded in a bright environment due to the extraneous light entering the objective A room light immediately above the microscope may also enter the objective as extraneous light especially when using a condenser lens with a longer working distance i e ELWD and LWD In this case it is recommended t...

Page 11: ...the ports that are not in use 7 Caution on motorized devices Do not force movements on the motorized parts by hand 8 Note on vibrations caused by motorized operations This product is designed to minimize the amount of vibration during motorized operation However depending on the application the vibration may have an effect on the microscopy 9 Note on the Z axis position display Ti E and Ti E B mic...

Page 12: ... Power Supply A for 220 240V 25 1 9 AC Adapter 26 2 Microscopy 27 2 1 Introduction to Microscopy 28 2 2 Bright Field BF Microscopy 30 2 3 Phase Contrast Ph Microscopy 38 2 4 External Phase Contrast Microscopy 43 2 5 In focus Observation with PFS 49 3 Operation 53 3 1 Power On Off 54 3 1 1 Power On 54 3 1 2 Power Off 54 3 2 Dia Illumination Operation 55 3 2 1 Combination of Lamp Dia Pillar Illumina...

Page 13: ...14 PFS Perfect Focus System Operation 81 3 14 1 PFS Overview 81 3 14 2 Starting and Stopping In focus Observation with the PFS 84 3 14 3 Offset Adjustment 86 3 14 4 Registration and Restoration of Offset 87 3 14 5 If the Objective is Changed 88 3 14 6 If the PFS Function Does Not Start 88 4 Assembly 89 5 Troubleshooting 118 5 1 Image Viewing 118 5 2 Operation 120 5 3 Electrical System 120 5 4 Perf...

Page 14: ...e used in specific combinations Do not use these devices in an inappropriate combination For combinations of the lamp the dia pillar illuminator and the power supply device refer to page 55 For details on microscopy procedures see Chapter 2 Microscopy For details on the operation of each part see Chapter 3 Operation If the microscope has not been assembled yet first refer to Chapter 4 Assembly Whe...

Page 15: ...V30W MAX 12V100W L80 EY E DIS PLAY ME MO RY PFS ON RECALL Z R ESE T BR IGHT NE SS R1 00 L100 FO CU S Figure 1 2 Ti E left view Attach a protective cap on the side port when the side port is not in use Left operation panel Left side port Focus knob Dia illumination lamphouse Figure D LH LC Filter sliders both sides 4 total Dia pillar illuminator Figure TI DH Condenser mount Nosepiece Figure TI ND6 ...

Page 16: ... Obj ON OFF 6V30W MAX MIN 12V100W Figure 1 4 Microscope base left view Epi illumination field diaphragm slider mount Focus knob Left side port Left operation panel Dia pillar illuminator mount A carrying handle is attached for transportation Connector panel rear Right side port Focus knob Bottom port on the bottom Ti E B only Front operation panel Status display panel Right operation panel Eyepiec...

Page 17: ...i E B EYE L100 R100 B100 BRIGHTNESS switch display brightness selector Status display panel 20x2 DISPLAY switches display item selector Obj switch objective selector Coarse Fine ExFine switch indicator focus knob resolution selector indicator Dia illumination lamp ON OFF switch Dia illumination lamp brightness control knob Focus knob Epi Shutter switch epi illumination shutter operation Coarse Fin...

Page 18: ...any interference received including interference that may cause undesired operation This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numØrique de la classe A est conforme la norme NMB 003 du Canada Figure 1 9 Microscope base rear view Epi fl illumination mount DC24V IN connector for AC adapter MIC CONTROL connector for Hub Controller A PFS connector for PFS motorized nos...

Page 19: ...YE Figure 1 12 TI T BPH Eyepiece Base Unit for External Phase Contrast This is an eyepiece tube base for external phase contrast microscopy A turret is provided at the front to attach external phase plates Three positions A to C are available Focus settings can be adjusted for three positions individually Besides phase plates can be centered with the screws at both side and clamped with the screw ...

Page 20: ...mic Eyepiece Tube B Figure 1 15 TI TERG Ergonomic Eyepiece Tube This is an eyepiece tube with a tilt mechanism for user s physical attribute This eyepiece tube has a manual shutter and a Bertrand lens The optical path for the binocular part can be blocked with the shutter for photomicrography Besides the objective pupil can be observed when the Bertrand lens is placed in the optical path Eyepiece ...

Page 21: ...SE Figure 1 17 PFS Offset Controller The offset controller body is magnetically attached to the base plate Use caution when lifting PFS Offset Controller as the base plate may become detached and fall DICHROIC MIRROR IN OUT lever dichroic mirror operation lever Optical component housing Do not place items on the box Do not apply excessive force Objective sockets x6 DIC prism ports x6 Interface cab...

Page 22: ...in stage comes with the following two concentric rings 25 mm bore with oiling notch 40 mm bore TI SAM Attachable Mechanical Stage Figure 1 20 Attachable Mechanical Stage on Plain Stage This stage is attached to the plain stage when using specimen holders The specimen can be moved in the X and Y directions by operating the stage knob Various specimen holders can be used by attaching the following a...

Page 23: ...he TI DS Dia Pillar Illuminator 30W has a built in lamphouse but requires a separate condenser The following illustration shows the TI DS Dia Pillar Illuminator 30W with the ELWD S Condenser EN TI DS 30W 6V 30W HALOGEN Figure 1 22 TI DS Dia Pillar Illuminator 30W with ELWD S Condenser D LH LC Precentered Lamphouse LC Requires TI PS100W power supply Lamp cable Condenser mount rotatable Condenser mo...

Page 24: ...ar Illuminator 30W Figure 1 24 TI C Condenser Turret mounted on TI DS Dia Pillar Illuminator 30W The system condenser can be mounted on the TI DS Dia Pillar Illuminator 30W for HMC observation only Condenser focus knob Condenser focus knob Condenser centering knob Condenser lens 4 types ELWD LWD HMC and CLWD Condenser cassette clamp screws both sides of cassette Condenser turret Condenser cassette...

Page 25: ...e 1 25 ELWD S Condenser The ELWD S Condenser supports bright field microscopy and phase contrast microscopy It can be used with both 100W and 30W dia pillar illuminators Condenser lens Turret Centering knob Clamp screws both sides Condenser centering knobs both sides ...

Page 26: ...knob on the microscope for output voltage control When this switch is turned off the brightness control knob on the front of the power supply becomes enabled and the brightness control knob on the microscope becomes disabled EXTERNAL external control signal input connector Connect the control cable to this connector and the LAMP CTRL connector on the rear of the microscope 1 2 6 8 7 3 4 5 Pin Sign...

Page 27: ... the connector for connecting the power supply device to a wall outlet Be sure to use the specified power cord for the connection CTRL external control ON OFF switch Turn this switch on to use the brightness control knob on the microscope for output voltage control When this switch is turned off the brightness control knob on the front of the power supply becomes enabled and the brightness control...

Page 28: ...e microscope is supplied via an AC adapter The AC adapter can be used with 100 to 240 VAC at 50 60 Hz and can be used with most wall outlets in the world Use the AC adapter included with the product Use a power cord specified in Chapter 7 Specifications AC inlet AC adapter DC output cable ...

Page 29: ...ng Ti Control refer to the Ti Control instruction manual When using Ti E or Ti E B with TI HUBC A Hub Controller A attached on the back Refer to the instruction manual provided with TI HUBA B Hub Controller A The TI HUBC A Hub Controller A is used to control the motorized components When the TI HUBC A Hub Controller A is attached to the back of the microscope operation procedures for the motorized...

Page 30: ...ce Tube B Bright field microscopy see 2 2 Phase contrast microscopy without external phase contrast devices see 2 3 External phase contrast microscopy see 2 4 In focus observation with PFS see 2 5 For the name and position of each part refer to Chapter 1 Part Names For details on operation methods refer to Chapter 3 Operation If the microscope has not been assembled yet first refer to Chapter 4 As...

Page 31: ...le configuration 1 Ti E microscope body 2 AC adapter power cord 3 TI T B Eyepiece Base Unit 4 TI TD Eyepiece Tube B 5 CFI 10x eyepiece x2 6 TI DH Dia Pillar Illuminator 100W filters NCB11 ND4 GIF 7 D LH LC Precentered Lamphouse LC 12V 100W Halogen Lamp 8 TI PS100W Power Supply power cord 9 TI C Condenser Turret LWD Condenser Lens condenser cassette for bright field microscopy condenser cassette fo...

Page 32: ...for a better image 1 Reset convenient functions Page 31 2 Turn on the dia illuminator Page 31 3 Adjust the illumination for optimal color reproduction Page 32 4 Prepare the optical path Page 32 5 Set up for bright field microscopy Page 33 7 Adjust the diopters and the interpupillary distance Page 34 8 Re adjust the focus Page 34 9 Center the condenser Page 35 10 Observe the specimen Page 36 11 Cha...

Page 33: ...essing the I side of the POWER switch on the power supply 3 Turn on the Ti E microscope by pressing the ON side of the POWER switch on the back of the microscope The Ti E microscope is turned on and the current status is displayed on the status display panel 4 Turn on the lamp by pressing the dia illumination lamp ON OFF switch on the left side of the microscope The display content of the status d...

Page 34: ...ive is in the optical path Display example for objective 10x NA 0 25 ____________10x 0 25 E100_Coarse__PFS Out 2 Direct a 100 light towards the eyepiece observation port by pressing the EYE switch on the front operation panel The selected switch will light up in green and the port name E100 will be displayed on the status display panel Display example for output port Z ____0 000um E100_Coarse 3 Fu...

Page 35: ...field of view 3 Look into the eyepiece Adjust the focus onto the specimen by using the Coarse Fine ExFine switches and the focus knobs on the sides of the microscope Use the Coarse Fine ExFine switches to change the focus knob resolution speed of vertical movement for easier focus adjustment There are three resolutions Coarse Fine and ExFine extra fine The current setting will be displayed by an i...

Page 36: ... Obj switch 5 Look into the left eyepiece with your left eye Adjust the focus onto the specimen by rotating the diopter adjustment ring on the left eyepiece Do not touch the focus knobs at this time 6 Repeat steps 2 through 5 two times 7 Adjust the right eyepiece Repeat steps 2 through 6 but this time using the right eyepiece instead of the left 8 Adjust the interpupillary distance of the binocula...

Page 37: ... the microscope 2 Rotate the field diaphragm knob on the dia pillar illuminator until the field diaphragm image is visible in the field of view 3 Adjust the focus onto the field diaphragm image by rotating the condenser focus knob on the dia pillar illuminator 4 Move the field diaphragm image to the center of the field of view by turning the two condenser centering screws on the dia pillar illumin...

Page 38: ...ition B This will allow you to observe the objective pupil plane and the aperture diaphragm image Adjust the focus by rotating the Bertrand lens focusing knob on the right side of the eyepiece tube and then adjust the size of the aperture diaphragm image to 70 80 the size of the objective pupil plane When done move the Bertrand lens out of the optical path by moving the operation lever to position...

Page 39: ...press the Refocus switch without rotating the focus knobs Condenser refocusing clamp If there is a need to move the condenser up and down tighten the condenser refocusing clamp before moving the condenser This will make it easier to restore the condenser to the original position 12 End the observation Coa rse Fine ExF ine Obj ON OFF 6V30W MAX 12V10 L80 EYE DISPLAY MEMORY PFS ON RECALL Z RESE T BRI...

Page 40: ...cal path Center the position of the condenser annular diaphragm in the condenser cassette so that it is aligned with the phase plate ring in the objective 1 Adjust the focus onto the specimen with BF microscopy Page 39 2 Set up for phase contrast microscopy Page 39 3 Center the condenser annular diaphragm Page 40 4 Observe the specimen Page 40 5 Change the objective Page 41 6 Change the specimen P...

Page 41: ...t If the aperture diaphragm is not fully open the optical path of the annular diaphragm will overlap with the aperture diaphragm and the phase contrast effect cannot be achieved Be sure to fully open the aperture diaphragm when performing phase contrast microscopy 4 Fully open the aperture diaphragm by rotating the aperture diaphragm knob on the dia pillar illuminator clockwise to the limit When u...

Page 42: ...oarse Fine ExFine Epi Shutter FL Block Refocus Escape PFS OFFSET OUT DICHRO IC MIRROR IN L80 EYE MEMORY DISPLAY ON Z RESET 1X 1 5X BRIGHT NESS R100 L100 FOCUS PFS 1 Check that the aperture diaphragm of the condenser is fully open 2 Adjust the size of the field diaphragm image to closely match the size of the field of view by rotating the field diaphragm knob on the dia pillar illuminator 3 Move th...

Page 43: ...e Fine ExFine Epi Shutter FL Block Refocus Escape PFS OFFSET OUT DICHRO IC MIRROR IN L80 EYE MEMORY DISPLAY ON Z RESET 1X 1 5X BRIGHT NESS R100 L100 FOCUS PFS Use the following functions as necessary Inclining of dia pillar illuminator When using the 100W dia pillar illuminator the entire dia pillar illuminator can be inclined backward by loosening the fixing knob on its back side so as to secure ...

Page 44: ... off the dia illumination by pressing the dia illumination lamp ON OFF switch on the left side of the microscope 2 Turn off the microscope by pressing the OFF side of the POWER switch on the back of the microscope 3 Turn off the power supply by pressing the O side of the POWER switch on the back of the power supply If placing a cover on the microscope wait until the lamp has cooled sufficiently 7 ...

Page 45: ...y can now be performed with normal objectives without a phase ring and phase contrast objectives are no longer required Figure 2 3 Optical path for external phase contrast microscopy A camera port is also provided on the external phase contrast optics system enabling photomicroscopy of phase contrast images For example a primary image plane camera port i e side port of an inverted microscope can b...

Page 46: ... 1 Ph3 LWD CLWD P Apo 60x WI NA1 2 60x Ph3 2 Ph3 LWD CLWD P Apo VC 60x WI NA1 2 60x Ph3 3 Ph3 LWD CLWD P Apo VC 60x H NA1 4 60x Ph3 4 Ph4 CLWD Apo TIRF 60x H NA1 49 60x Ph4 5 Ph4 CLWD P Apo TIRF 60x H NA1 45 60x Ph4 6 Ph3 LWD CLWD P Apo VC 100x H NA1 4 100x Ph3 7 Ph4 CLWD Apo TIRF 100x H NA1 49 100x Ph4 External phase contrast microscopy workflow Outline Place the objective refer to the table abov...

Page 47: ...ular diaphragm will overlap with the aperture diaphragm and the phase contrast effect cannot be achieved 4 Fully open the aperture diaphragm by rotating the aperture diaphragm knob on the dia pillar illuminator clockwise to the limit 5 Move the Bertrand lens into the optical path by moving the Bertrand lens in out lever on the front of the eyepiece tube to position B Adjust the focus onto the exte...

Page 48: ... Coarse Fine ExFine Epi Shutter FL Block Refocus Escape PFS OFFSET OUT DICHRO IC MIRROR IN L80 EYE MEMORY DISPLAY ON Z RESET 1X 1 5X BRIGHT NESS R100 L100 FOCUS PFS S I D E EY E 1 Fully open the aperture diaphragm of the condenser 2 Adjust the size of the field diaphragm image to closely match the size of the field of view by rotating the field diaphragm knob on the dia pillar illuminator 3 Move t...

Page 49: ...MIRROR IN L80 EYE MEMORY DISPLAY ON Z RESET 1X 1 5X BRIGHT NESS R100 L100 FOCUS PFS S I D E EY E Use the following functions as necessary Inclining of dia pillar illuminator When using the 100W dia pillar illuminator the entire dia pillar illuminator can be inclined backward by loosening the fixing knob on its back side so as to secure more working space Escape switch Refocus switch If there is a ...

Page 50: ...urn off the dia illumination by pressing the dia illumination lamp ON OFF switch on the left side of the microscope 2 Turn off the microscope by pressing the OFF side of the POWER switch on the back of the microscope 3 Turn off the power supply by pressing the O side of the POWER switch on the back of the power supply If placing a cover on the microscope wait until the lamp has cooled sufficiently...

Page 51: ...ual this method will be referred to as in focus observation or PFS observation NOTE 2 NOTE 1 The boundary surface is also referred to as an interface NOTE 2 PFS Perfect Focus System In focus observation workflow Outline Perform bright field microscopy or phase contrast microscopy with the TI ND6 PFS PFS Motorized Nosepiece To perform in focus observation you must register the objective information...

Page 52: ...h an IR filter infrared filter to the filter sliders on the pillar illuminator and move it into the optical path PFS uses near infrared light for focus management Be sure to place the IR filter into the optical path to minimize the effect of the heat rays infrared light emitted by the illumination 3 Display the PFS information on the status display panel by pressing the DISPLAY switch on the front...

Page 53: ... focus slightly 3 Start the PFS function by pressing the PFS ON switch on the front operation panel The PFS ON switch will be lit and the PFS information will change to PFS On PFS control started ____________10x 0 25 E100_Coarse__PFS On 4 Observe the specimen P F S D O W N U P P F S OFFSET CONTROLLER FINE COARSE 1 The observation surface can be moved up and down without losing the focus by rotatin...

Page 54: ...n the top of the PFS Motorized Nosepiece to the OUT position The PFS information display will change to PFS Out Dichroic mirror Out ____________10x 0 25 E100_Coarse__PFS Out Be sure to stop the PFS function before changing the specimen or ending the observation 6 End the observation Coa rse Fine ExF ine Obj ON OFF 6V30W MAX 12V10 L80 EYE DISPLAY MEMORY PFS ON RECALL Z RESE T BRIGHTNES S R100 L100 ...

Page 55: ...t function properly unless the objective information has been registered correctly For details on using Ti Control refer to the Ti Control instruction manual This chapter describes the operation methods for each part of the product For the name and position of each part refer to Chapter 1 Part Names For the microscopy procedure refer to Chapter 2 Microscopy If the microscope has not been assembled...

Page 56: ...evice complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 EQUIPMENT 4N75 MODEL Ti E INSPECTION TUV Rheinland Product Safety JAPAN this device must accept any interference received including interference that may cause undesired operation This Class A digital apparatus complies with Canadian ICES 003 Cet ...

Page 57: ...0W EN TI DS 30W 6V 30W HALO GEN Both power supply units have the same appearance 3 2 2 Power Supply Setting ON OFF E X T E R N A L MADE IN JAPAN MODEL TI PS100W Figure 3 3 Power supply rear view The EXTERNAL switch or CTRL switch on the back of the power supply can be set to allow switching dia illumination lamp on off and controlling brightness from the left operation panel of the microscope If t...

Page 58: ... the voltage is decreased the light becomes dark and reddish White light When accurate color reproduction is critical set the brightness adjustment knob to the 6V30W or 12V100W position depending on the lamp used then move the NCB11 filter into the optical path This setting will provide the whitest light To adjust the brightness adjust the ND filters on the dia pillar illuminator 3 2 4 Brightness ...

Page 59: ... position display after setting the focus you will be to use the set position as a reference point For details refer to Section 3 10 Focusing Mechanism Operation 5 PFS control switches and indicator These controls are used for in focus observation with the PFS Perfect Focus System For details refer to Section 3 14 PFS Operation FOCUS indicator The indicator shows the in focus status When the objec...

Page 60: ...Apo_TIRF 000x 0 00 Apo TIRF Apo TIRF Plan 000x 0 00 Plan Plan P_Apo 000x 0 00 P Apo Plan Apo P_Apo_TIRF 000x 0 00 P Apo TIRF Plan Apo TIRF P_Apo_VC 000x 0 00 P Apo VC Plan Apo VC P_Fluor 000x 0 00 P Fluor Plan Fluor Plan_UW 000x 0 00 Plan UW Plan UW S_Fluor 000x 0 00 S Fluor S Fluor S_P_Fluor 000x 0 00 S P Fluor S P Fluor HMC 000x 0 00 HMC HMC Other 000x 0 00 Other Other information Objective stat...

Page 61: ... switch for a clockwise rotation or the rear switch for a counterclockwise rotation TI HUBC A Hub Controller A is required to use the TI ND6 E Motorized Sextuple DIC Nosepiece TI N6 Sextuple Nosepiece and TI ND6 Sextuple Nosepiece are manually operated and cannot be controlled by the Obj switch 4 Dia illumination lamp ON OFF switch Turns the dia illumination on off Push the switch in to turn on th...

Page 62: ...ized shutter for the epi illumination The Epi Shutter switch becomes enabled when TI HUBC A Hub Controller A and a motorized shutter for epi illumination are attached For details refer to the instruction manual provided with Hub Controller A 4 FL Block switch filter block selector Changes the filter block in the optical path by rotating the motorized filter turret The FL Block switch becomes enabl...

Page 63: ...100 Figure 3 10 Ti E B optical path selector switches Switch Light distribution 1 EYE Eyepiece observation port 100 2 L100 Left side port 100 3 R100 Right side port 100 4 B100 Bottom port 100 3 may be set to R80 right side port 80 eyepiece observation port 20 or L80 left side port 80 eyepiece observation port 20 depending on your purchased configuration 3 4 3 Eyepiece Base Unit Options Figure 3 11...

Page 64: ...otomicroscopy ND Neutral Density Reduces the light intensity to 1 16 Transmittance Approx 6 NCB11 Corrects the color temperature for normal microscopy or filming by daylight type color TV cameras NCB Neutral Color Balance Color reproducibility is optimal when this filter is placed in the optical path and the lamp voltage is matched to the lamp rating Keep this filter out of the optical path when f...

Page 65: ... the image sensor as it may result in vignetting The field diaphragm of the TI DS Dia Pillar Illuminator 30W has a fixed opening size It cannot be adjusted Field diaphragm adjustment Move field diaphragm image into center of field of view Adjust its size to match field of view Figure 3 14 Field diaphragm adjustment 1 Move the 10x objective into the optical path 2 Rotate the field diaphragm knob on...

Page 66: ...agm open close lever to the left to reduce the aperture size or to the right to increase the aperture size Adjust the aperture diaphragm so that the size of the diaphragm image is at 70 80 of the size of the objective pupil plane Adjustment with Bertrand lens on eyepiece tube B Figure 3 17 Adjustment with Bertrand lens When using TI TD Eyepiece Tube B or TI TERG Ergonomic Eyepiece Tube use the Ber...

Page 67: ...e contrast microscopy with the TI C Condenser Turret If the aperture diaphragm is not fully open the optical path will be blocked The aperture diaphragm of the ELWD S condenser is used only for bright field microscopy This aperture diaphragm is independent of the phase contrast annular diaphragm Adjustment of the aperture diaphragm will not affect the annular diaphragm ...

Page 68: ...ntroller A Phase contrast microscopy When performing phase contrast microscopy use a condenser cassette that has the same Ph code as the objective and the external phase contrast ring and center the annular diaphragm after moving the condenser cassette into the optical path Be sure to fully open the aperture diaphragm If the aperture diaphragm is not fully open the optical path will be blocked Com...

Page 69: ... 3 20 ELWD S Condenser The ELWD S Condenser supports bright field microscopy and phase contrast microscopy The ELWD S Condenser can be used with both 100W and 30W pillar illuminators Condenser lens Turret Condenser centering knob both sides Centering knob clamp screw both sides ...

Page 70: ...path 6 Look into the left eyepiece with your left eye Adjust the focus onto the specimen by rotating the diopter adjustment ring on the left eyepiece Do not touch the focus knobs at this time 7 Repeat steps 3 through 6 two times 8 Adjust the right eyepiece Repeat steps 2 through 7 but this time using the right eyepiece instead of the left 3 9 2 Interpupillary Distance Adjustment Figure 3 22 Interp...

Page 71: ...by moving the Bertrand lens operation lever at the lower front to position B Rotate the Bertrand lens focusing knob on the right to adjust the focus This will allow you to view the objective pupil plane a bright circle and the aperture diaphragm image Position Optical element Description O Blank Use to observe the microscope image with the eyepiece tube B Bertrand lens Use to observe the objective...

Page 72: ...ocus adjustment is approximately 7 5 mm upward and approximately 2 5 mm downward from the reference position Focus knob resolution selector The resolution for focus knob rotation can be set to one of the following three levels Setting Distance traveled per rotation Coarse 300 μm per rotation reference value Fine 50 μm per rotation ExFine extra fine 6 25 μm per rotation When set to COARSE mode the ...

Page 73: ...m E100_Coarse Figure 3 26 Z RESET switch operation The Z axis position of the focus mechanism displayed on the status display panel increases when the nosepiece is elevated and decreases when the nosepiece is lowered Once you have set the focus at the desired height pressing the Z RESET switch on the front operation panel and resetting the Z axis position display will allow you to use the set posi...

Page 74: ... to record the current position and lower the objective to the retracted position The objective can be brought back up to the focused position by pressing the Refocus switch making refocusing easier This function is useful for temporarily moving the objective out of the way for example when replacing the specimen or changing the objective When the objective is at the retracted position its height ...

Page 75: ...that is thicker than the supported glass thickness i e when observing a specimen in a Petri dish it is recommended that you use an objective with a correction ring so that the optical system can be adjusted accordingly See Section 3 11 3 Objectives with Correction Ring Glass thickness of 0 17 mm Figure 3 31 Glass thickness of 0 17 mm When using an objective labeled 0 17 face the cover glass downwa...

Page 76: ... bottom plate For the thickness of the bottom plate take an actual measurement or refer to the specifications provided by the manufacturer An acrylic concentric ring is useful as it will allow you to view the operating parts from above the stage as you work 2 Focus on the specimen by rotating the focus knobs 3 If the resolution and contrast of the image are poor slightly rotate the correction ring...

Page 77: ... 2 Taking care not to let bubbles form apply the bare minimum amount of oil onto the tip of the objective If too much oil is applied the excess oil may overflow onto the stage and other parts Use as little oil as possible just enough to fill the space between the objective tip and the specimen and take care not to allow the oil to get on other parts 3 Place the specimen onto the stage 4 Slowly rai...

Page 78: ...leum benzine wipe with absolute alcohol ethyl or methyl for a better finish If petroleum benzine is unavailable use methyl alcohol However as methyl alcohol does not clean as well as petroleum benzine it will be necessary to wipe a few more times Three to four wipes are usually sufficient When wiping oil off of the specimen take care not to damage the specimen Caution Residual oil on an oil immers...

Page 79: ...imen with deionized or distilled water Caution Do NOT use tap water If tap water is used impurities may adhere to and solidify on the lens causing the lens to become scratched when being cleaned Plan Apo 60xWI NA 1 2 is equipped with a correction ring to achieve optimal aberration on cover glasses of different thicknesses A reading of 17 on the scale indicates 0 17 mm When using a cover glass meas...

Page 80: ...nser focus knob to the limit This function is useful for use with high magnification condensers or when the pillar cannot be inclined The condenser refocusing clamp has a range of motion of 13 mm 3 12 2 Condenser Mount Rotation Figure 3 39 Condenser mount The condenser mount can be rotated if the condenser mount rotation clamp screw is loosened Use this function to adjust the orientation of the tu...

Page 81: ...r into and out of an incline take caution not to get your hands and fingers caught in the hinge When attaching relatively heavy objects to the pillar be sure to secure them properly A loose screw may result in the attachment falling off when the pillar is inclined In particular be sure to properly secure high intensity lamphouses and lamphouse adapters 3 12 4 Device Attachment Screw Holes Figure 3...

Page 82: ...25 mm bore with oiling notch 40 mm bore Figure 3 43 Concentric rings Two concentric rings of different sizes are provided with the microscope ø25 mm and ø40 mm Use whichever is appropriate depending on the size of the specimen When using the 40 mm ring the objective may collide with the bottom surface of the stage if the nosepiece is rotated with the stage moved out of the observation area In such...

Page 83: ...n continuously observing or photographing changes in living cells as it is not affected by changes in the specimen or by discoloration under fluorescent light and is capable of maintaining the focus by compensating for minor focus shifts that may occur with time or by stage movements NOTE 1 When using a dry objective the boundary surface between the cover glass and air will be detected NOTE 2 The ...

Page 84: ... difficult to capture the relatively small amount of reflective light from the boundary surface 3 Specimens with large amounts of scattered or reflected light For the same reason as for the sliced specimens non sliced specimens that scatter a large amount of light are also unsupported 4 Cells attached to a container with a glass bottom thicker than 170 μm When using a dry objective with a specimen...

Page 85: ...0 72 Dry Plan Apo 60xWI 1 2 0 22 WI Plan Fluor ELWD 40xC 0 6 3 7 2 7 Dry Plan Apo DM 60xHA 1 4 0 21 Oil Plan Fluor ELWD ADL 40xC 0 6 3 7 2 7 Dry Plan Apo DM 60xH 1 4 0 21 Oil Plan Fluor ELWD DM 40xC 0 6 3 7 2 7 Dry Plan Apo 100xH 1 4 0 13 Oil Plan Fluor 100x H 1 3 0 2 Oil Plan Apo DM 100xH 1 4 0 13 Oil Plan Fluor ADH 100x H 1 3 0 19 Oil Plan Apo TIRF 60xH 1 45 0 13 Oil Plan Fluor DLL 100x H 1 3 0 ...

Page 86: ... filter into the optical path For epi illumination attach an IR filter ø25 mm to the filter sliders on the epi illuminator 3 Place the dichroic mirror into the optical path by moving the DICHROIC MIRROR IN OUT lever on the top of the PFS Motorized Nosepiece to the IN position 4 Focus on the specimen by rotating the focus knobs on the microscope 5 Start the PFS function by pressing the PFS ON switc...

Page 87: ...or details refer to Section 3 14 3 Offset Adjustment MEMORY PFS ON RECALL FOCUS MEMORY PFS ON RECALL FOCUS Figure 3 49 Stopping the PFS function 7 To stop the PFS function press the illuminated PFS ON switch The PFS ON switch indicator turns off and the PFS function stops Be sure to stop the PFS function before replacing the specimen or changing the objective 8 When observing without using the PFS...

Page 88: ...ARSE Figure 3 50 PFS Offset Controller PFS Offset Controller operation The PFS offset controller has an offset dial component and a base plate that are magnetically attached By changing the orientation of the dial component on the base plate the dial can be faced to the left or the right The dial can also be faced upward if used alone without the base plate DO WN U P PFS OFFSET CONTROLLER FINE COA...

Page 89: ...essed The offset can be registered independently for each objective on the nosepiece max 6 The registered offsets will be retained until the system is turned off Restoring the offset MEMORY PFS ON RECALL FOCUS Figure 3 53 Restoring the offset 1 Check that the MEMORY switch is lit 2 Press the RECALL switch The offset lens automatically moves to the registered offset position Manual operation with t...

Page 90: ... into the optical path by moving the dichroic mirror operation lever on the top of the PFS nosepiece to the IN position Even if the focus is set on the boundary surface the focus may not be set on the observation target Adjust the offset as necessary as described in Section 3 14 3 Offset adjustment In particular when using a dry objective difference in cover glass thickness may cause the initial p...

Page 91: ...th care and avoid subjecting it to strong physical shocks In particular the accuracy of objectives may be lost by even weak physical shocks Caution Notes on setup To use the PFS Motorized Nosepiece you will need to register the objective information on the microscope After assembling the product connect a PC and set up the microscope before use For setup use the Ti Control setup software for Ti se...

Page 92: ...e the power supply and contact Nikon Use of a power supply with the incorrect voltage rating may result in malfunctions electric shock or fire Assembly diagram Stage Eyepiece tube Eyepiece base unit Power supply device To a wall outlet To a wall outlet Nosepiece Objective Eyepieces Main body microscope base TI DH Dia Pillar Illuminator 100W and system condenser configuration TI DS Dia Pillar Illum...

Page 93: ... of two or more people When lifting the microscope base hold it securely by the recess at the bottom front and the carrying handle at the rear 3 Remove the clamping bolt A clamping bolt is attached to the left side of the body to secure the product during transportation Remove the clamping bolt with the provided 4 mm hex screwdriver and attach the provided rubber cap to the clamping bolt hole If p...

Page 94: ...roscope base There are two positioning pins on the bottom of the eyepiece base unit Align these pins with the holes on the microscope base 2 Secure the eyepiece base unit by tightening four hex socket head screws with the provided 4 mm hex screwdriver Selecting objectives and phase plates The phase plate must be selected to suit the objective being used Refer to the following table to select an ob...

Page 95: ...eyepiece base unit Screw in the phase plate into the socket on the turret 4 Remove the special tool and affix the provided label below the indication on the front of the turret 5 Repeat steps 1 thru 4 for positions A thru C as necessary Phase plate replacement 1 Select position A B or C by rotating the turret on the external phase contrast eyepiece base unit 2 Insert the special tool into the opti...

Page 96: ...etail joint on the bottom of the eyepiece tube into the female circular dovetail joint on the eyepiece base unit 3 Tighten the eyepiece tube clamp screw with a 2 mm hex screwdriver Check that the eyepiece tube and the eyepiece base unit are fixed securely 4 Attach the eyepieces to the binocular part of the eyepiece tube When inserting align any one of the three grooves on the eyepiece to the protr...

Page 97: ...t the center of the microscope base 2 Press the PFS Motorized Nosepiece from the front to the rear and secure it by tightening the two M5 hex socket head screws provided with the nosepiece Be sure to use washers with the hex socket head screws 3 Guide the cable from the PFS Motorized Nosepiece through the groove on the microscope base to the back of the microscope Be sure to guide the cable throug...

Page 98: ...e orientation of the nosepiece and place it on the rectangular groove on the focusing part at the center of the microscope base 2 Press the nosepiece from the front to the rear and secure it by tightening the two M5 hex socket head screws provided with the nosepiece Be sure to use washers with the hex socket head screws Do not attach the objectives until the stage has been attached Hexagonal socke...

Page 99: ...s described below Figure 4 16 Protection Plate for the PFS6 Nosepiece attachment 1 Using a 2 mm hex screwdriver provided with Ti E or Ti E B remove the four M3 hex socket head screws from the top of the FL turret as shown in the figure at left 2 Attach the Protection Plate for the PFS6 Nosepiece onto the top of the FL turret Insert and tighten the four M3 hex socket head screws that were removed i...

Page 100: ...wo of four M3 hex socket head screws from the top of the filter turret TI FLC TI FLC E or TI FLC E HQ as shown in the figure at left 2 Attach the Protection Plate for the Nosepiece onto the top of the FL turret Insert and tighten the two M3 hex socket head screws that were removed in step 1 3 Attach the nosepiece TI N6 TI ND6 or TI ND6 E to the Ti E or Ti E B microscope body and secure with the 4 ...

Page 101: ... the positioning pin 2 Using the 4 mm hex screwdriver secure the dia pillar illuminator by tightening the four M5 hex socket head screws provided with the dia pillar illuminator 3 Attach the condenser mount to the dia pillar illuminator 1 Remove the fall stop screw 2 Attach the condenser mount by sliding it onto the dovetail groove on the dia pillar illuminator Slide the mount upward to the limit ...

Page 102: ...the cable clamp on the back of the dia pillar illuminator The cable clamp is attached to the dia pillar illuminator with the hooks on its sides To remove the cable clamp push in the hooks from the sides The cable clamp can hold up to four cables 5 Attach the provided ferrite core on the connector end of the lamp cable 1 Snap open the ferrite core 2 Wrap the lamp cable once around the ferrite core ...

Page 103: ... illuminator by tightening the four M5 hex socket head screws provided with the dia pillar illuminator 3 Attach the provided ferrite core on the pillar end of the lamp cable 1 Snap open the ferrite core 2 Wrap the lamp cable once around the ferrite core so that the cable passes through the ferrite core twice Adjust the position of the ferrite core so that it is approximately 20 to 30 mm from the p...

Page 104: ... the tab of the filter slider 3 Insert the filter slider into the slot of the dia pillar illuminator The filter slider has latches that determine the limit of slide operation Press the latches up and press the filter into the slot You can insert up to four filter sliders for 100 W type illuminator and three filter sliders for 30 W type illuminator You can insert filters sliders from the right or t...

Page 105: ... the base of the dia pillar illuminator Align the holes on the bottom of the stage to these pins Stages with a stage movement knob are typically attached with the knob positioned in the far right However they can also be rotated by 180 degrees and be attached with the knob in the near left Figure 4 29 Stage attachment 2 Secure the stage by tightening the four M5 hex socket head screws provided wit...

Page 106: ...ge Figure 4 30 Objective attachment 1 Remove the concentric ring specimen holder and other equipment from the stage 2 Screw in an objective into a socket on the nosepiece through the opening in the stage Attach the objectives so that magnification is increased by rotating the nosepiece clockwise as viewed from above Nosepiece Objective ...

Page 107: ...a Pillar Illuminator 100W Figure 4 31 Lamphouse Cover Removal Figure 4 32 Lamp Replacement CAUTION HOT Do not touch the lamp and the lamphouse while the lamp is on or for thirty minutes after it has been turned off 1 Insert a hex wrench into the hole on the top of the lamphouse cover Loosen the clamp screw and remove the lamphouse cover 2 Push in the lamp clamp lever and remove the used lamp from ...

Page 108: ...crew on the back of the dia pillar illuminator and remove the limit plate from the rear cover 2 Open the rear cover on the back of the dia pillar illuminator by lifting it backward 3 Remove the used lamp from the socket 4 Insert a new lamp into the socket Be sure to use the specified lamp Do not touch the lamp glass with your bare hands 5 Close the rear cover 6 Re attach the limit plate onto the r...

Page 109: ... the condenser holder The condenser clamp screw is located inside the hole on the right side of the condenser holder If the condenser mount is shifted from the reference position the screw will not be visible in the hole In that case loosen the condenser mount rotation clamp screw align the positioning groove on the mount with the positioning pin on the condenser holder by rotating the mount and t...

Page 110: ...secure it with two hex socket head screws Up to five condenser cassettes can be attached to the turret Attach the cassettes so that their numbering is increased by rotating the turret clockwise as viewed from above 4 Screw in the condenser lens into the bottom of the turret For combinations of condenser lenses and condenser cassettes see Chapter 3 Operation 3 Attaching the ELWD S condenser Figure ...

Page 111: ...MC Condenser attachment system condenser 1 Attach the 52 mm polarization filter to the extension tube provided with the HMC condenser lens Threads are cut on the inside of the extension tube and on the outside of the polarization filter 2 Attach the extension tube to the dia pillar illuminator 3 Attach the condenser holder to the extension tube 4 Attach the turret on the system condenser to the co...

Page 112: ...amera device and then attach the camera device with the adapters into the side port adapter Attach a protective cap onto the port when the port is not in use Example Attaching the C mount TV camera to the direct C mount adapter 1 Screw the C mount TV camera securely into the direct C mount adapter 2 Insert the direct C mount adapter into the side port adapter and secure it by tightening the lockin...

Page 113: ... to the camera device and then attach the camera device with the adapters into the bottom port adapter Attach a protective cap onto the port when the port is not in use Example Attaching the C mount TV camera to the direct C mount adapter 1 Screw the C mount TV camera securely into the direct C mount adapter 2 Insert the direct C mount adapter into the bottom port adapter and secure it by tighteni...

Page 114: ...see page 101 MIC CONTROL POWER OFF ON LAMP CTRL DC24V IN USB Gepruftes Medizinprodukt Approved medical device Freiwillige Produktprufung R 5 3 1 0 0 1 This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 this device may not cause harmful interference and 2 EQUIPMENT 4N75 MODEL Ti E INSPECTION TUV Rheinland Product Safety JAPAN this device must a...

Page 115: ... DISPLA Y ON Z RESET 1X 1 5X BRIGH TNESS R100 L100 FOCUS PFS P F S DO W N UP P F S OFFSET CONTROLLER FINE COARSE Figure 4 45 PFS Offset Controller connection When using the PFS Motorized Nosepiece connect the PFS Offset Controller to the Ti E or Ti E B microscope body Connect the cable from the PFS Offset Controller to the OFFSET connector on the right side of the microscope body PFS Offset Contro...

Page 116: ...ired operation This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numØrique de la classe A est conforme la norme NMB 003 du Canada Figure 4 47 Ti E Ti E B rear view Power to the microscope is supplied via an AC adapter The AC adapter can be used with 100 to 240VAC at 50 60Hz and can be used with most wall outlets in the world 1 Check that the POWER switch on the Ti E or Ti...

Page 117: ...mote Control Pad the objective information can be registered from the remote control pad For details refer to the instruction manual provided with the TI RCP Remote Control Pad 1 Preparing the software To register the objective information on the microscope install the following software on a commercial PC Nikon Ti Control The Ti Control software and its instruction manual can be downloaded from t...

Page 118: ...are installation details refer to the instruction manual of the software 3 Start the installed software and register the information for the objectives attached to the PFS Motorized Nosepiece Information for PFS compatible objectives is pre registered on the software For each socket on the nosepiece specify the objective to be used 4 Save the registered information The settings are stored on the b...

Page 119: ... BRIGHTNESS R100 L100 FOCUS PFS P F S D O W N U P P F S OFFSET CONTROLLER FINE COARSE Figure 4 50 Ti E Dia illumination lamphouse Figure D LH LC Filter sliders both sides 4 total Dia pillar illuminator Figure TI DH Condenser mount Nosepiece Figure TI ND6 PFS Stage knob Right side port Focus knob PFS Offset Controller Front operation panel Power supply device Figure TI PS100W Eyepiece base unit Fig...

Page 120: ...lement or on the culture vessel Clean the optical elements Use a clean culture vessel Dirt or dust on the lens or other optical element or on the culture vessel Clean the optical elements Use a clean culture vessel Dirt or dust is seen in the field of view Field diaphragm image not focused on focal plane of specimen Focus and center the condenser correctly Dirt or dust on the lens or other optical...

Page 121: ...e dia pillar illuminator upright until it reaches the limit NCB11 filter not in optical path Move the ND filter into the optical path Yellow tinged image Lamp voltage too low Adjust brightness control knob to match lamp rating ND filter not in optical path Move the ND filter into the optical path Field of view too bright Lamp voltage too high Lower lamp voltage with the brightness control knob Ope...

Page 122: ...h the power switch is set to ON The AC adapter or the power cord for the power supply is not connected at all or is not connected securely Turn off the power switch and check connection of AC adapter and power cord The dia illuminator lamp does not light Dead lamp Replace with a specified lamp Dia illuminator lamp burns out in a short time Unsupported lamp used Replace with a specified lamp Contro...

Page 123: ...wer Disconnect and reconnect the PFS Offset Controller MEMORY switch pressed during offset recall operation Retry after offset recall operation is complete Offset cannot be registered by pressing the MEMORY switch No beep Objective in the optical path does not support PFS Panel display ER1 Register the objectives correctly and move an appropriate objective into the optical path Offset cannot be re...

Page 124: ...face not detected within time limit Panel display ER2 When using a dry objective PFS is turned off for safety if no boundary surface is detected within 5 seconds PFS dichroic mirror not in the optical path Panel display ER3 PFS is turned off for safety Move dichroic mirror into the optical path and turn on PFS again Nosepiece is placed out of a click stop position Panel display ER4 PFS is turned o...

Page 125: ...lammable Handle with care Do not use near an open flame or operate a power switch in the vicinity When using absolute alcohol follow the instructions provided by the manufacturer 6 2 Cleaning the Microscope Body Use silicon cloth to clean the microscope body For persistent dirt dampen a piece of gauze with neutral detergent and wipe gently Do not use organic solvents They may cause discoloration o...

Page 126: ...vary depending on how fast the knob is rotated D LH LC Precentered Lamphouse LC Input ratings 12 VDC 100 W Lamp ratings 12 V 100 W halogen lamp Specified lamp model Halogen lamp OSRAM HLX 64623 or PHILIPS 7724I Average lamp life 2000 hours TI PS100W Power Supply Input ratings 100 to 240 VAC 10 1 8 A 50 60 Hz Built in fuse ratings 250 V T4A Output ratings 12 VDC 100 W Maximum output current 8 4 A E...

Page 127: ... μm per rotation When set to COARSE mode the distance traveled by rotating a focus knob will vary depending on how fast the knob is rotated TI DS Dia Pillar Illuminator 30W Input ratings 6 VDC 30 W Lamp ratings 6 V 30 W halogen lamp Specified lamp model Halogen lamp PHILIPS 5761 Average lamp life 100 hours TE PS30 Power Supply A TE PSE30 Power Supply A Input ratings TE PS30W 100 to 120 VAC 10 50 6...

Page 128: ... Note 1 Some objectives offer a wider offset range Note 2 The following objectives feature special offset ranges S Fluor DL 40xC 30 to 10 μm Plan Apo 40x 30 to 10 μm Plan Apo DM 40x 30 to 10 μm Plan Apo TIRF 60xH 5 to 0 5 μm Apo TIRF 60xH 5 to 0 5 μm Focusing performance Focusing time 700 msec or less at a position near the focal point at the boundary surface Focusing accuracy 1 3 or less of the f...

Page 129: ...4 0 13 Oil Plan Fluor 10x 0 3 16 Dry Plan Fluor DLL 10x 0 3 16 Dry Plan Fluor ELWD 20xC 0 45 7 4 Dry Plan Fluor ELWD ADL 20xC 0 45 7 4 Dry Plan Fluor ELWD DM 20xC 0 45 7 4 Dry Plan Fluor 40x 0 75 0 72 Dry Plan Fluor 40xH 1 3 0 2 Oil Plan Fluor DLL 40x 0 75 0 72 Dry Plan Fluor ELWD 40xC 0 6 3 7 2 7 Dry Plan Fluor ELWD ADL 40xC 0 6 3 7 2 7 Dry Plan Fluor ELWD DM 40xC 0 6 3 7 2 7 Dry Plan Fluor 100xH...

Page 130: ...ndard 3 conductor grounding 3 conductor grounding Type H05VV F 2 5 m long maximum rated at 250V AC minimum For Japan PSE approved detachable power cord set 3 conductor grounding 3 conductor grounding Type VCTF 3x0 75mm2 1 8 m long maximum rated at 125V AC minimum 7 5 2 Power Cord for the Power Supply AC inlet and plug appearance For countries where the power supply is 100 to 120 VAC but not Japan ...

Page 131: ...n accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This product complies with Canadian EMI ICES 003 Class A This Class A digital apparatus complies with Canadian ICES 003 Cet app...

Page 132: ...33MM NCB11 Filter 33MM ND2 A Filter 33MM ND16 A D LH LC Lamphouse W LC TI PS100W PS100 240V TE PS30W PS A 115V TE PSE30 PS A 230V TI DS DIA Pillar 30W TI DH DIA Base Kit TE C ELWD S Condenser TE C ELWD S Condenser C HT Terasaki Holder C HSG Slide Glass Holder 35MM Petri Dish Holder Stage Ring Holder 96 Hole Plate Holder TI S E Motorized Stage TI S ER Motorized Stage RE TI BSUK70 Stage Up Kit TI FL...

Page 133: ...ystick TI HUBC A Hub TI AC A AC Adapter A TI RCP Remote Control Pad TI ERGO Ergo Controller C Mount CCTV Camera C Mount TV Adapter A TI FL FL Attachment TI SFL S EPI FL Attachment TI TIRF TIRF Illumination TI TIRF E Motorized TIRF TI TIRF Stage Up Lens C1 Single Mode Fiber TI LUSU Shutter Unit C HDFIA HG100W Adapter C HGFIF15 HG Fiber C HGFI HG Fiber Illuminator HMX Lamphouse Halogen Lamp Socket 1...

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Nikon Eclipse Ti-E, Instructions Manual

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