Chapter 2 Microscopy
2.2 Bright-Field (BF) Microscopy
33
5
Set up for bright-field microscopy.
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escap
e
PFS
OFFSE
T
OUT
DICHR
OIC MIR
ROR
IN
L80
EYE
MEMORY
DISPL
AY
ON
Z-RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
1. Move the condenser cassette for bright-field
microscopy into the optical path by rotating
the condenser turret to position “A”.
6
Set specimen and adjust the focus.
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z-RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
1. Place the specimen onto the stage.
2. Move the stage to bring the observation
target into the center of the field of view.
3. Look into the eyepiece. Adjust the focus onto
the specimen by using the Coarse/Fine/
ExFine switches and the focus knobs on the
sides of the microscope.
Use the Coarse/Fine/ExFine switches to change
the focus knob resolution (speed of vertical
movement) for easier focus adjustment. There
are three resolutions: Coarse, Fine, and ExFine
(extra fine). The current setting will be displayed
by an indicator.
Focus knob resolution and Z-axis position can be
displayed on the status display panel.
Display example for focus knob resolution
and Z-axis position
Z :
_
+ 1 2 4 . 2 2 5 u m
E 1 0 0
_
F i n e
The Z-axis position information on the status
display panel can be reset to zero by pressing the
Z-RESET switch on the front operation panel.
By pressing the Z-RESET switch when the focus
is on the specimen, you will be able to use the
Z-axis position value as reference when
readjusting the focus.
5-1
6-2
6-3
6-1
6-3
Summary of Contents for Eclipse Ti-E
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