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III.  Operation of each part

46

Cover glass thickness

On every objective, there is a mark indicating the thickness of the cover glass to be used with.    (“

  / 0.17” means to use

a cover glass of 0.17 mm thick.)    When observing with a “0.17” marked objective, place a specimen so that its cover glass
(0.17 mm thick) faces the objective.    (In case of an inverted microscope, set a specimen so that its cover glass faces
down.)    For an objective with a “1.2” mark, place a specimen so that its slide glass faces the objective, because the
normal slide glass thickness is 1.2 mm.    (In case of an inverted microscope, set a specimen so that its cover glass faces
up.)    When you observe a specimen in a laboratory dish or the like at high magnification through a glass not conforming
to the specified thickness, we recommend use of an objective that has a correction ring capable of correcting the glass
thickness error.

Oil immersion objective

The objective marked with “Oil” is an oil immersion objective.
Before using the oil immersion objective, be sure to immerse the space between the end of the objective and the specimen,
with the supplied oil (Nikon immersion oil).    Use the non-fluorescent oil (option) when you carry out fluorescent
microscopy using the oil immersion objective for fluorescent microscopy.
Keep out air bubbles from the oil, since they will deteriorate visibility of images.    Air bubbles may be found by observing
the exit pupil (bright circle) of the objective.    (To observe the exit pupil of the objective, turn the eyepiece tube turret to
position “B” and focus with the Bertrand lens focusing screw.    If your microscope does not have an eyepiece tube turret,
remove one eyepiece and insert the centering telescope with an adapter and turn the eyepiece part of the centering
telescope to focus.)
To remove air bubbles, rotate the revolving nosepiece slightly, and move the oil-immersed objective back and forth once
or twice.    Or, wipe the oil off, then reapply oil to the objective.
If excessive oil is applied, surplus oil flows out and adheres to the stage or other component.    Use a minimum necessary
amount of oil (enough to fill the space between the end of the objective and the specimen.    Use care not to put oil to any
other components.
If oil remains on the oil immersion objective or adheres to the surface of a dry objective, it will greatly reduce image
visibility.    After use, thoroughly wipe the oil off the objective surface.    Also make sure that no oil has spread to the
surfaces of other objectives.
To remove oil, moisten a lens tissue or clean cloth with petroleum benzine and lightly wipe the lens surface a few times.
Use a fresh part of a lens tissue every time.    For best results, wipe last with absolute alcohol (ethyl or methyl alcohol).
If you cannot obtain petroleum benzine, use methyl alcohol.    Because methyl alcohol is a weaker cleaning agent, you will
need to wipe the surface repeatedly.    (Usually, three or four wipes are adequate to clean the surface.)
Absolute alcohol and petroleum benzine are quite inflammable.    Use great care when handling them and when setting the
power switch on and off.    Be very careful with fire.
The use of acrylic stage ring (option) will facilitate the immersion operation with the culture dish left on the stage.    Set the
stage ring so that its opening runs along the rim of the revolving nosepiece.    Rotate the nosepiece till the objective shows
from the opening of the stage ring and hold the nosepiece there to immerse the top lens of the objective.

Water immersion objective

The objective marked with “WI” is a water immersion objective.    (The one with longer working distance is for the upright
type microscopes.)
Before using the water immersion objective, be sure to immerse the space between the end of the objective and the
specimen, with the demineralized or distilled water.    (Do not use the tap water for immersion since when it dries up,
impurities will stuck on the lens surface and may cause a scratch during cleaning.)
Plan Apo 60xWI objective (N.A. = 1.2) has a correction ring to compensate the difference of the cover glass thickness for
the best abberation compensation.    The mark “17” on the graduation shows the position for the 0.17 mm thick cover glass.
Before using the cover glass, measure its thickness with the micrometer or the like, and set the correction ring to match
that thickness for the best compensation.

Summary of Contents for ECLIPSE TE2000-E

Page 1: ...M314E 04 7 CF 3 1 5 Inverted Microscope ECLIPSE TE2000 E ECLIPSE TE2000 U ECLIPSE TE2000 S Instructions ...

Page 2: ......

Page 3: ...eiture of all claims against warranty For your safety read the instruction manual carefully and thoroughly before using the instrument Do not discard this manual but keep it near the product for easy reference In this manual safety instructions are indicated with the symbols shown below Be sure to follow the instructions indicated with these symbols to ensure correct and safe operation Symbol Mean...

Page 4: ...r in a well ventilated location To ensure that it radiates heat properly and does not overheat never cover or place any object on the adapter To prevent malfunction always turn off the power switch switch to O of the HUB controller before attaching the AC adapter Specified AC adapter Manufacturer PHIHONG ENTERPRISE Taiwan Model PSA30U 120 N Rated input voltage AC 100 240 V 0 7 A 50 60 Hz Voltage f...

Page 5: ...pen Do not break used lamps instead dispose of them as special industrial waste or according to the laws applicable to your municipal waste system 4 Do not wet the microscope If the instrument gets wet a short circuit may result that may cause malfunction or abnormal heating If you accidentally spill a liquid on the instrument immediately turn off the power switch flip to the side and unplug the p...

Page 6: ...as a room that receives direct sunlight Choose a location that is free from dust or dirt Do not install the microscope in a warm 60 C or more humid 85 or more location Mold or condensation will form on the lenses and filters Leave enough space against the nearby wall since the lamphouse will become hot by lamp illumination When using the T DH dia illuminator 100W leave a certain space between the ...

Page 7: ...nator 30W SLWD condenser and T TS eyepiece tube S 25 3 Microscope system consisting of TE2000 E dia illuminator 30W SLWD condenser and T TS eyepiece tube S 29 4 Photomicrography using a 35 mm camera mounted on the front port 34 III Operation of each part 37 1 Power ON OFF 38 2 Brightness adjustment 39 3 Optical path switching 40 4 Using filters 42 5 Using field diaphragm 42 6 Using condenser apert...

Page 8: ... controller which mounts on the rear side of the microscope allows the user to control attached motorized units For details refer to the instruction manual provided with the T HUBC HUB controller Dia illuminator P 13 Eyepiece tube P 7 Power supply P 17 Stage P 15 Microscope rear P 16 Microscope base P 8 Condenser P 14 This is a photograph of TE2000 U microscope with T DH dia illuminator 100W LHS H...

Page 9: ... Open B Bertrand lens with focusing screw C Close Shutter M 2 5X magnifier T TS eyepiece tube S A Diopter adjustment ring B Eyepiece T TERG ergonomic eyepiece tube A Diopter adjustment ring B Eyepiece C Eyepiece tube turret O Open B Bertrand lens with focusing screw C Close Shutter T TI intermediate tube A Clamp screw for holding various devices B Mount for various devices such as Trinocular eyepi...

Page 10: ...eyepiece tube Eyepiece tube clamp screw Front port REFOCUS switch Raises the objective to the memorized position Photo mask dial Clockwise turn OUT Counterclockwise turn IN Intermediate magnification dial 1X No intermediate magnification 1 5X 1 5X intermediate magnification for all ports Fine focus knob The distance of travel achieved by one rotation of the knob can be set to Fine Middle or Coarse...

Page 11: ...jective refocusing ring Forward turn Release Backward turn Lock TE2000 U right side TE2000 U left side Dia illumination ON OFF switch Brightness adjustment dial This dial is disabled when the remote control pad is used for bright adjustment Stage Objective Mount for cassette holder For the installation of the cassette holder for epi fl microscopy Left side port Black cover for elevating section Re...

Page 12: ... illumination ON OFF switch Brightness adjustment dial This dial is disabled when the remote control pad is used for bright adjustment Stage Objective Mount for cassette holder For the installation of the cassette holder for epi fl microscopy Left side port Black cover for elevating section Remove the cover when installing the epi fl attachment Mount for analyzer Install the analyzer here for DIC ...

Page 13: ...5 can be mounted T BDCA direct C mount adapter Digital still camera for the microscopes such as DXM1200 can be mounted here Various adapters for side port Side port adapter This adapter is supplied with the microscope T BPA photo adapter Double port adapter Various TV adapters Various adapters for bottom port TE2000 U except for the bottom port type and TE2000 S are not equipped with bottom port B...

Page 14: ...f the focus knob main unit and the base plate which are connected by a magnet The direction of the T EFN focus knob can be switched between right and left by changing the main unit s position relative to the base plate Note that the focus adjustment knob can be operated independently with the knob facing up Knob rightward rotation clockwise Raises the objective brings the objective closer to the s...

Page 15: ... D Condenser focus knob E Condenser clamp screw F Condenser mount positioning pin G Condenser mount positioning groove H Condenser mount rotation clamp screw I Condenser centering screw J Condenser mount rotatable K LHS H100P 1 12V100W lamphouse L Lamphouse clamp screw M Lamphouse cover clamp screw N Condenser holder removable O Condensr holder clamp screw P Lamp cable Q Condenser holder fall stop...

Page 16: ... for HMC observation A Condenser aperture diaphragm B Condenser module C Condenser module clamp screw D Condenser lens 3 types available ELWD LWD HMC E Annular diaphragm centering screw on Ph modules only ELWD S condenser SLWD condenser A Centering handle clamp screw B Centering handle C Turret A B C SLWD condenser can only be mounted on T DS dia illuminator 30W ...

Page 17: ...I Parts of the microscope and their names 15 5 Stages focusing module T SR rectangular stage A Stage ring B Y axis stage movement knob C X axis stage movement knob T SP plain stage A Stage ring ...

Page 18: ...b is supplied with the TE2000 E only SHUTTER Refer to VI 21 Connection of external equipment in the instruction manual supplied with the HUB controller STAGE Refer to VI 21 Connection of external equipment in the instruction manual supplied with the HUB controller PC Connect a PC EXP Refer to VI 22 Connection to the EXP connector in the instruction manual supplied with the HUB controller TE PS Con...

Page 19: ...brightness adjustment dial on the microscope or the DIA LAMP adjustment key on the T RCP remote control pad when the T DH dia illuminator 100W is used Turning this switch OFF activates the brightness adjustment dial on the power supply Pilot lamp Turns on when the power is on Pin 2 ditto TE PS30 power supply for 100 120 V area TE PSE30 power supply for 220 240 V area Pin 3 Lamp ON OFF Input Open O...

Page 20: ...TE2000 U or TE2000 S with the T HUBC HUB controller mounted on the rear For the microscopy procedure refer to the instruction manual supplied with the T HUBC HUB controller The T HUBC HUB controller which is mounted on the rear of the microscope controls the operations of all attached motorized units Note that when the T HUBC HUB controller is mounted on the rear of the microscope the microscope c...

Page 21: ...oscope system consisting of TE2000 S dia illuminator 30W SLWD condenser and T TS eyepiece tube S 2 1 Bright field microscopy 2 2 Phase contrast microscopy 3 Microscope system consisting of TE2000 E dia illuminator 30W SLWD condenser and T TS eyepiece tube S 3 1 Bright field microscopy 3 2 Phase contrast microscopy 4 Photomicrography using a 35 mm camera mounted on the front port Microscope system ...

Page 22: ...e Objectives for bright field microscopy Objectives for phase contrast microscopy Others 1 1 Bright field BF microscopy Key point Detach all optical elements required for other types of observation from the optical path Position the condenser correctly adjust the focus and centering and adjust the clarity of the image by moving the aperture diaphragm 1 Reset the convenient functions 1 Turn the con...

Page 23: ...perture diaphragm fully 5 Turn the condenser focus knob to lower the condenser mount to the lowest position Supplementary information When the system condenser is mounted with the ELWD condenser lens position the condenser mount at the location about 1 cm below the uppermost position When using the ELWD S condenser position the condenser mount at the location about 2 cm below the uppermost positio...

Page 24: ...about the same as that of the view field 7 Turn the two condenser centering screws on the dia illuminator to move the field diaphragm image to the center of the view field Field of view Field diaphragm image Field of view Field diaphragm image 9 Conduct observation 1 Select an objective of desired magnification 2 Move the aperture diaphragm lever on the system condenser to set the opening to 70 to...

Page 25: ...rret and set it to the same Ph code as that of the objective placed in the optical path in step 1 3 Turn the aperture diaphragm lever on the condenser to the right limit to open the aperture diaphragm fully Supplementary information If the aperture diaphragm is not fully open the optical path of the annular diaphragm will be restricted preventing the realization of the appropriate phase contrast e...

Page 26: ...w field Supplementary information If precise color fidelity is not required the brightness can be adjusted by varying the lamp voltage using the brightness adjustment dial on the left side of the microscope 5 Conduct observations using an objective with a different magnification value 1 Move a different Ph objective of desired magnification into the optical path 2 Turn the condenser turret and set...

Page 27: ...m of the SLWD condenser is fixed There is no aperture diaphragm 1 Turn on the dia illumination 1 Make sure that the input voltage indication on the rear side of the power supply corresponds with the voltage of the commercial power source in the area If different do not turn on the switch Contact your nearest Nikon representative immediately 2 Turn on the CTRL switch on the rear side of the power s...

Page 28: ...X objective into the optical path 5 While looking into the left eyepiece with your left eye turn the diopter adjustment ring of the left eyepiece to bring the specimen into focus Do not touch the fine coarse focus knob on the microscope main body in this step 6 Repeat steps 2 through 5 two more times 7 Adjust the right side by following the same procedure Substitute right for left in steps 2 throu...

Page 29: ... the PhL diaphragm into the optical path 3 Remove one eyepiece and insert the centering telescope using the adapter 4 Turn the eyepiece of the centering telescope to bring the annular diaphragm image into focus 5 Turn the two annular diaphragm centering screws on the condenser so that the annular diaphragm image coincides with the phase plate image in the objective 6 Reinstall the eyepiece to the ...

Page 30: ...ove a different Ph objective of desired magnification into the optical path 2 Slide the Ph annular diaphragm slider to set to the same Ph code as that of the objective placed in the optical path in step 1 6 Upon the completion of microscopy Follow the same procedure as for bright field BF microscopy ...

Page 31: ...servation from the optical path The size of the field diaphragm of the SLWD condenser is fixed There is no aperture diaphragm Since the 6V30W lamp ON OFF and lamp voltage adjustment cannot be controlled using the T RCP remote control pad use the switch and control on the microscope main body 1 Turn on the dia illumination 1 Make sure that the input voltage indication on the rear side of the power ...

Page 32: ...ine coarse focus knob to bring the specimen into focus 5 Adjust the diopter and distance between the eyepieces 1 Turn the photo mask dial on the front side of the microscope counterclockwise to move the photo mask into the optical path 2 Look into the left eyepiece with your left eye Turn the diopter adjustment ring of the left eyepiece to bring the double crosshairs of the photo mask into focus 3...

Page 33: ...Supplementary information If precise color fidelity is not required brightness can be adjusted by varying the lamp voltage using the brightness adjustment dial on the left side of the microscope 7 Upon the completion of microscopy 1 Turn off the power switch of the HUB controller Flip the switch to 2 Turn off the power switch of the power supply Flip the switch to 3 After the lamp mounting section...

Page 34: ...l path 2 Slide the Ph annular diaphragm slider to move the PhL diaphragm into the optical path 3 Remove one eyepiece and insert the centering telescope using the adapter 4 Turn the eyepiece of the centering telescope to bring the annular diaphragm image into focus 5 Turn the two annular diaphragm centering screws on the condenser so that the annular diaphragm image coincides with the phase plate i...

Page 35: ...the remote control pad to move a different Ph objective of desired magnification into the optical path 2 Slide the Ph annular diaphragm slider to set to the same Ph code as that of the objective placed in the optical path in step 1 6 Upon the completion of microscopy Follow the same procedure as for bright field BF microscopy ...

Page 36: ... D CFI 10X eyepiece T DH dia illuminator 100W LHS H100P 1 12V100W lamphouse 12V100W halogen lamp TE2 PS100W power supply System condenser LWD condenser lens Condenser module for bright field microscopy Condenser module for phase contrast microscopy T SR rectangular stage T N6 sextuple nosepiece Objectives for bright field microscopy Objectives for phase contrast microscopy Others 1 Focus on the sp...

Page 37: ...front side of the microscope counterclockwise to move the photo mask into the optical path 2 Turn the fine coarse focus knob on the microscope to bring the specimen into focus Supplementary information Setting the eyepiece tube turret to position M facilitates the focusing process Take care not to touch the diopter adjustment ring of the eyepiece in this step 3 Adjust the position of the specimen ...

Page 38: ...formation If precise color fidelity is not required such as when monochromic film is used the brightness may be reduced by using the brightness adjustment dial on the left side of the microscope 7 Prevent the entry of extraneous light 1 Set the eyepiece tube turret to position C 2 Install the finder cap to the camera finder or cover the finder with a cloth Supplementary information If an eyepiece ...

Page 39: ...ption of each part If you are using the TE2000 E model read the instruction manual supplied with the T HUBC HUB controller When the T HUBC HUB controller is mounted on the rear side of the microscope read the instruction manual supplied with the HUB controller Before using the instrument read the and Notes on handling the microscope at the beginning of this manual and be sure to follow the warning...

Page 40: ...12V100W lamphouse is needed TE2 PS100W For 100 240V areas TE PS30 For 100 120V areas TE PSE30 For 230V areas TE2000 TE2000 E TE2000 U TE2000 S 6V30W Halogen lamp PHILIPS 5761 T DS 30W The remote control pad and PC cannot be used to control the lamp ON OFF and lamp voltage adjustment Power ON OFF There is a power switch at the front of the power supply Pressing the switch to side will turn ON the p...

Page 41: ...umination To make the illumination dark turn the dial to smaller number which results in giving the reddish hue to the illumination On normal use set the dial between 6 to 12 If the color reproducibility is of concern such as when taking pictures using day light type color film set the dial to the same indication as the ratings of the lamp and place the NCB11 filter in the optical path The illumin...

Page 42: ...ort Right side port Left side port 1 100 2 20 80 3 1 4 20 80 5 100 Equipment that can be attached to the port Eyepiece tube Intermediate Tube trinocular eyepiece tube various equipment SLR camera F mount digital camera Photomicro graphy equipment for microscopes Digital camera TV camera Photomicro graphy equipment for microscopes Digital camera TV camera 1 Dial position 3 cannot be used on normal ...

Page 43: ...ed out the optical path is switched from the observation port to the bottom port If the bottom port is not used be sure to press in the bottom port changeover lever The optical paths to any other ports can be switched in the same manner as for the TE2000 U For TE2000 S Light distribution Position of optical path switchover dial Observation port Left side port SIDE 20 80 EYE 100 Equipment that can ...

Page 44: ...graphy HA Heat absorption filter For reducing the influence of heat rays in the illumination against specimen Though the dia illuminator has a built in heat absorption filter set this filter in the optical path if your specimen is very sensitive to heat 5 Using field diaphragm The field diaphragm restricts the illumination on a specimen to an observed area Raising the field diaphragm lever to its ...

Page 45: ...g the lever to the right opens it Adjust so that the diaphragm reaches 70 to 80 of the exit pupil of the objective T TD eyepiece tube D or T TERG ergonomic eyepiece tube Turn the eyepiece tube turret to B to move the Bertrand lens into the optical path Turn the Bertrand lens focusing screw to observe an image of the objective s exit pupil bright circle and the aperture diaphragm Other eyepiece tub...

Page 46: ...r example a Ph condenser for Ph microscopy and a DIC condenser for DIC microscopy The system condenser of this microscope features modules that contain the optical elements Up to five modules can be incorporated into the unit As a result the user needs only to rotate the turret in order to execute various microscopic applications without changing condensers Modules can be arranged freely in the tu...

Page 47: ...or compensation for even thickness Adjusting the correction ring 1 Turn the correction ring to match the bottom plate thickness of the container The thickness should be a measured value or the value stated by the manufacturer of the container The acrylic stage ring is available to improve working efficiency since it enables the part of operation to be seen from above the stage 2 Focus on the speci...

Page 48: ...ecessary amount of oil enough to fill the space between the end of the objective and the specimen Use care not to put oil to any other components If oil remains on the oil immersion objective or adheres to the surface of a dry objective it will greatly reduce image visibility After use thoroughly wipe the oil off the objective surface Also make sure that no oil has spread to the surfaces of other ...

Page 49: ... position 3 Bring the 40x objective in the optical path 4 Look into the right eyepiece with your right eye Turn the focus knob to focus on the specimen 5 Bring the 4x or 10x objective in the optical path 6 Look into the right eyepiece with your right eye turn the diopter adjustment ring on the right eyepiece to focus on the specimen Do not touch the focus knob at this time 7 Repeat steps 3 to 6 tw...

Page 50: ... in focus Once the refocusing ring is clamped at the in focus position refocusing is much easier after shifting the objective for specimen change or other purpose All you have to do is rotate the coarse focus knob until it reaches the limit The refocusing ring is useful if for example the objective is very close to the stage because the container of the specimen has a thick bottom plate and the ma...

Page 51: ...the opposite direction then turn once again in the original direction 12 T DH dia illuminator 100W Condenser refocusing clamp can be used only when LWD condenser lens is attached After focusing the field diaphragm image on the specimen surface using the condenser focus knob turn the condenser refocusing clamp clockwise to mark that position Even if the condenser is raised for specimen replacement ...

Page 52: ...he upper two holes are for the devices that should be moved away from the stage when the dia illuminator is tilted The lower two holes are for the devices which should remain on the stage when the dia illuminator is tilted 13 T SR rectangular stage Normally mount the stage so that its handle comes to the rear right of the microscope It is also possible to mount the stage in the diagonally opposite...

Page 53: ...es of the view field become darker 35mm SLR camera F mount that have aperture opening priority auto mode A such as FM3A FE10 FE2 FE F100 F90 F70 F801 series F601 series F501 F5 F4 F3 etc Front port T BSLR SLR camera adapter 2 5X Digital still camera for microscopes DXM1200 DN100 etc Front port Side port Bottom port T BDCA direct C mount adapter Photomicrographic equipment for microscopes U III H I...

Page 54: ...ecified port you will need to switch back to the observation port frequently 4 Check the trimming For TE2000 E TE2000 U Use the photo mask For TE2000 S Attach the mask eyepiece instead of an eyepiece and check or look through the finder on the camera or photomicrography equipment 5 Focus on the film surface For TE2000 E TE2000 U Turn the focus knob so that both the photo mask and the specimen imag...

Page 55: ...ret It should be noted however that the range shown by the mask eyepiece is only a rough guide If precise trimming is required look and check through the finder on the camera or the photomicrography equipment The following illustration shows the range taken on photo when the photomicrography equipment is attached on the side port The range differs according to the magnification of the projection l...

Page 56: ...cratches or fingerprints on lenses will adversely affect the image Assemble the microscope so as not to put scratches on the lenses The microscope is a precision optical equipment Handle carefully and do not subject it to strong shocks The precision of the objectives in particular can be adversely affected even by weak shocks When using the TE2000 E When mounting the T HUBC HUB controller on the r...

Page 57: ...ator Filter slider Condenser holder Lamp 4 1 4 2 9 4 5 8 7 Combination of T DS dia illuminator 30W and ELWD S condenser Checking the power supply voltage Make sure that the input voltage indicated on your power supply corresponds with the voltage of the commercial power supply in your area If the two values do not match do not use the power supply and contact your nearest Nikon representative imme...

Page 58: ...andle from the base Place the dia illuminator oo the rear mount Make sure that the positioning pins on the mount fit into the holes on the illuminator Fix the illuminator with four M5 hexagonal head bolts supplied together If T DH dia illuminator 100W is to be used do the following also 1 Attach the condenser mount Attach the condenser mount sliding it from the bottom to the top along the dovetail...

Page 59: ...d immediately after the lamp is distinguished When exchanging the lamp turn off the lamp and wait till the lamp cools sufficiently Do not touch the lamp with bare hands Dusts and fingerprints on the lamp surface will lead to uneven illumination and shortening of the lamp life Use gloves When using T DH dia illuminator 100W W W W Remove the cover of the lamphouse by loosening the clamp screw on the...

Page 60: ...ottom mount of the condenser holder so that the indication A faces front Fix the turret securely by the condenser clamp screw 3 Insert the modules to the turret and fix each of them by two hexagonal screws Five modules can be attached to the turret Set them so that the indication on the module increases as the turret is rotated clockwise when seen from above 4 Screw on the condenser lens to the bo...

Page 61: ...or is to be connected to the OUTPUT connector on the power supply Do not forget to tighten the lock ring on the connector 2 Control cable Connect one side the LAMP CTRL connector on the microscope and the other side to the EXTERNAL connector on the power supply If TE PS30 or TE PSE30 power supply is used connect to CTRL connector instead of the EXTERNAL connector When installing the HUB controller...

Page 62: ...ttempt to rotate the camera in the direction of the arrow Positioning groove Adapter clamp screw 11 Side port See page 51 for the photomicrography devices that can be attached to the side port and the adapters needed 1 Remove the plastic cap on the side port by loosening the clamp screw 2 Insert the side port adapter and fix it by the clamp screw 3 Insert the adapter to the side port adapter and f...

Page 63: ...ontainer Objective s correction ring not set to the bottom plate thickness of the container Adjust correctly Bottom plate thickness of the container is outside the correction range of the objective Use container with bottom plate thickness within correction range Poor image quality Poor contrast Poor resolution Field diaphragm image not focused on the specimen surface Perform condenser focusing an...

Page 64: ... 2 Operational Symptoms Causes Countermeasures Stage mounted incorrectly Mount it correctly Image not in focus although the objective is raised to the highest position Objective refocusing ring clamped Release the clamp by turning the ring counterclockwise to the limit Cannot focus with 20x and 40x objective Bottom plate thickness of the container is outside the correction range of the objective U...

Page 65: ...pe does not work EXTERNAL switch on the power supply CTRL switch when TE PS30 TE PSE30 is used is OFF Turn ON Lamp burnt Replace with specified lamp Dia illumination ON OFF switch on the microscope does not work Control cable not connected Connect properly Brightness adjustment dial on the power supply does not work EXTERNAL switch on the power supply CTRL switch when TE PS30 TE PSE30 is used is O...

Page 66: ... the power switch on off etc Follow the instructions provided by the manufacturer when using absolute alcohol 2 Cleaning the microscope We recommend that you use a silicon cloth to clean the microscope For persistent dirt dampen a piece of gauze with neutral detergent and wipe lightly Using organic solvents could result in discoloration of the plastic parts 3 Disinfecting the microscope We recomme...

Page 67: ...ut ratings of T DH dia illuminator 100W DC 12 V 100 W Lamp ratings 12V100W halogen lamp Lamp type Halogen lamp OSRAM HLX 64623 or PHILIPS 7724I Average lamp lifetime 2 000 hrs TE2 PS100W power supply Input ratings AC 100 240 V 50 60 Hz 2 4 A Voltage fluctuation 10 Output ratings DC 12 V 100 W 8 4 A Built in fuse ratings 250 V T4A Protection class Class 1 Others UL Recognized product GS approved Op...

Page 68: ... μm TE2000 E 1 μm TE2000 U S Input ratings of T DS dia illuminator 30W DC 6 V 30 W Lamp ratings DC 6V30 W halogen lamp Lamp Halogen lamp PHILIPS 5761 Average lamp lifetime 100 hrs TE PS30 power supply TE PSE30 power supply Input ratings TE PS30 AC 100 120 V 50 60 Hz 0 6 A TE PSE30 AC 230 V 50 60 Hz 0 4 A Voltage fluctuation 10 Output ratings DC 6 V 30 W Built in fuse ratings 250 V F2AH Protection ...

Page 69: ...ut current 2 5 A Others UL Listed product GS approved CE satisfied 4 Power cord for power supply and for AC adaptor of HUB controller Be sure to use the following power cords When the supply voltage is 100 to 120 V UL Listed detachable cord set 3 conductor grounding Type SVT No 18 AWG 3 m long maximum rated at 125 V AC minimum When the supply voltage is 220 to 240 V Approved according to EU EN sta...

Page 70: ...area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A est conforme à la norme NMB 003 du Canada Note that the microscope will not meet the above standards if used in combination with a motorized XY stage or shutter made b...

Page 71: ...VII Technical specifications 69 Blank Page ...

Page 72: ...ge Riser T HUBC Hub Controller T RCP Remote Controller T AC AC Adapter 2 Stages 1 Eyepiece Tubes 6 Nosepieces 7 Illumination Pillars 8 Epi fl Attachments 4 Side Port A B D T SAM Attachable Mechanical Stage T SP Plain Stage 2 Stages Acrylic Stage Ring Y TT Trinocular Tube TUW Centering Telescope T TD Eyepiece Tube D T TS Eyepiece Tube S Teaching Heads Y TF Trinocular Tube FUW Eyepiece Tubes Y IDT D...

Page 73: ...p Socket U Transformer Epi fl Collector Lens Q2 C FC Epi fl Collector Lens T FLC E Motorized Cassette Holder System Condenser Turret Provided with the HMC lens Remote Cable Remote Cable TE AT Double Lamphouse Adapter T DS Dia illuminator 30W T DH Dia illuminator 100W T FLC Cassette Holder Epi fl Filter Blocks T FL Epi fl Attachment T FL E Epi fl Attachment with Motorized Shutter T FLA FL Illuminat...

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