II. Microscopy
26
3.
Adjust the optical path.
1) Move the < 10X > objective into the optical path.
2) Set the “optical path switchover dial” on the right side of the
microscope to position < EYE >. (-> Direct 100% of the
light to the observation port.)
3) Place a specimen on the stage.
4.
Set the microscope for bright-field microscopy.
1) Slide the Ph annular diaphragm slider to move the < Open
> position into the optical path.
2) While looking into the eyepieces, turn the fine/coarse focus
knob to bring the specimen into focus.
5.
Adjust the diopter and interpupillary distance.
1) Turn the “diopter adjustment rings” of both right and left
eyepieces so that the engraved line of each diopter
adjustment ring aligns with the outer ring rim of the
eyepiece. (This sets the diopter adjustment to the "0"
position.)
2) Move the < 40X > objective into the optical path.
3) While looking into the left eyepiece with your left eye, turn
the “fine/coarse focus knob” of the microscope to bring the
specimen into focus.
4) Move the < 10X > objective into the optical path.
5) While looking into the left eyepiece with your left eye, turn
the “diopter adjustment ring” of the left eyepiece to bring
the specimen into focus. (Do not touch the fine/coarse
focus knob on the microscope main body in this step.)
6) Repeat steps 2) through 5) two more times.
7) Adjust the right side by following the same procedure.
(Substitute "right" for "left" in steps 2) through 5), and
repeat these steps three times.)
8) Adjust the interpupillary distance to consolidate the view
fields of both eyepieces.
6.
Conduct observation.
1) Select an objective of desired magnification.
2) Move the “ND filter” on the dia-illuminator in and out of the
optical path to adjust the brightness of the view field.
Supplementary information
If precise color fidelity is not required, the brightness
can be adjusted by varying the lamp voltage using the
“brightness adjustment dial” on the left side of the
microscope.
7.
Upon the completion of microscopy
1) Turn off the power switch of the power supply. (Flip the
switch to
{
.)
2) After the lamp mounting section cools, place the vinyl cover
on the microscope.
Engraved
line
4-2)
4-4)
4-3)
3-2)
Outer
ring rim
Diopter
adjustment ring
4-1)
4-5)
4-8)
