18
Capturing Multistained Images:
Cross Talk Reduction
(Standard Detector)
5
Chapter 5. Capturing Multistained Images: Cross Talk Reduction (Standard Detector)
5.1
Perform Steps 4.1 to 4.6 in Chapter 4, “Capturing Color Images”.
5.2
Set a channel series.
(1) Select [Custom] from the [Ch series].
(2) Click the [Custom] button to open the Line Channel Series Setup dialog box.
(3) In the Line Channel Series Setup dialog, set the order of scanning for each channel.
Setting the order of scanning arbitrarily:
Set the order of scanning channels using the channel scanning order matrix.
Using line sequence:
Select the [1->4] or [4->1] button.
Apply lasers to each scan line and start scanning.
Note: Be sure to set the TD (transmitted image) scanning order so that the scanning order comes
together with other channels because single TD scan is disabled. (Example: Laser is applied to
Ch3 and TD by second scanning.)
Fluorescence wavelength
TD
Channel
scanning order matrix
Scanning order
Line sequence
Channel names