Chapter 27. Capturing Simultaneous Photo Activation Imaging (Resonant & Galvano Scanner)
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(2) Set the photo activation time settings.
Wait:
Set the waiting time until activation starts.
Interval:
Set the time interval of photo activation.
ROIs:
Set stimulation groups used for activation.
Duration:
Set the duration time of photo activation.
When [Loops] is set, duration is automatically determined.
Loops:
Set the number of photo activation execution times.
(3) Set the time series time settings.
Duration:
Set the duration time of image acquisition.
When [Loops] is set, duration is automatically determined.
Loops:
Set the number of image acquisition execution times.
(4) Check the [Save to File] checkbox to acquire images while saving them.
Note: Images are saved in nd2 file format.
Note: We recommend acquiring time series images while saving them.
(5) Click
to read the settings for photo activation simultaneous imaging.
(6) Click the
[Run now] button to start photo activation simultaneous imaging.
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(3)
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(6)