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CONTENTS
Chapter 1. Introduction ............................................................................................................................................... 3
Basic Operation ............................................................................................................................................... 4
Chapter 2. Startup....................................................................................................................................................... 5
Chapter 3. Operation of the Microscope ..................................................................................................................... 7
Chapter 4. Capturing Color Images (Standard Detector) .......................................................................................... 13
Chapter 5. Capturing Multistained Images: Cross Talk Reduction (Standard Detector) ............................................ 18
Chapter 6. Capturing Confocal Zoom ....................................................................................................................... 22
Chapter 7. Capturing ROI Scan and CROP Scan..................................................................................................... 24
Chapter 8. Capturing Z Series Images ..................................................................................................................... 27
Chapter 9. Capturing Z Series Images (While Changing Brightness) ....................................................................... 30
Chapter 10. Creating Three-Dimensional Image ........................................................................................................ 32
Chapter 11. Creating a Slice View Image and a Projection Image ............................................................................. 35
Chapter 12. Capturing Time Series Images................................................................................................................ 36
Chapter 13. Time Measurement ................................................................................................................................. 38
Chapter 14. Capturing Photo Activation Imaging (Galvano Scanner / Time-Series Activation)................................... 40
Chapter 15. Image Display Function .......................................................................................................................... 43
Chapter 16. Extracting ND2 Files ............................................................................................................................... 47
Chapter 17. Exporting ND2 Files ................................................................................................................................ 48
Chapter 18. Shutdown................................................................................................................................................ 50
Spectrum Imaging ......................................................................................................................................... 51
Chapter 19. Capturing Spectral Images (Spectral Detector)....................................................................................... 52
Chapter 20. Separating Spectral Image (Unmixing) ................................................................................................... 57
Chapter 21. Live Unmixing (Spectral Unmixing for Live Image) ................................................................................. 61
Chapter 22. Capturing Virtual Filter Images (Spectral Detector)................................................................................. 63
Motorized Stage ............................................................................................................................................. 69
Chapter 23. Capturing Multipoint Time Series Images ............................................................................................... 70
Chapter 24. Capturing Large Images ......................................................................................................................... 72
High-Speed Imaging ...................................................................................................................................... 77
Chapter 25. Capturing High-Speed Images (Resonant Scanner)............................................................................... 78
Chapter 26. Capturing High-Speed ZT Series Images (Resonant Scanner) .............................................................. 82
Chapter 27. Capturing Simultaneous Photo Activation Imaging (Resonant & Galvano Scanner)............................... 86