2017-07 MAN-C0034-06
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NanoString Technologies
®
USER MANUAL
Enter the following information to create a Run Set:
1. nCounter Assay Type
– If the user has access to more than one nCounter assay type, the assay that was previously selected during
sign in will be displayed (
FIGURE 4.2
). To change this selection, the user must log out, then sign in again and select a different assay
type.
2. Run Set ID
– The Run Set ID must provide a unique name to identify the Run Set.
3. Test Configuration Code
– The Test Configuration Code is an alphanumeric barcode located inside the CodeSet box. It determines the
number of samples that can be processed.
4. CodeSet Kit Number
– The CodeSet Kit Number is a numeric barcode located inside the CodeSet box and is also described as a
CodeSet Barcode Sticker. It determines the CodeSet expiration date. Because there may be a delay between creating a Run Set and
processing the samples, a warning will be provided if the CodeSet is within two weeks of its expiration date when the Run Set is
created.
5. Sample Data
– The Reference Sample is always located in wells 1 and 2; they do not require any input and cannot be edited. Well
numbers 3-12 are used for patient RNA samples. Fields may vary for different assay types. As an example, Prosigna® requires the
following information:
a. Sample ID Label
(required) – Sample IDs can be entered using barcoded sample tubes and a barcode scanner connected to the
computer. If a scanner is not available or if the barcodes are damaged, sample IDs can be manually entered using a keyboard.
NanoString recommends using unique sample IDs to track samples.
b. # of Positive Nodes
(required) – Users can choose between zero positive nodes, 1-3 positive nodes or >=4 positive nodes (where
available).
c. Tumor Size
(required) – Users can choose between <= 2 cm or > 2 cm.
d. Memo
(optional) – Any notes about the sample can be entered here. There is a maximum limit of 32 characters.
NOTE:
Leave the remaining fields blank if any strip tube wells are not needed. If additional fields are required for more samples,
use a different test configuration that accommodates more samples.
IMPORTANT:
Some handheld scanners can misinterpret barcodes if they are not properly configured. It is essential that
the Test Configuration Code and CodeSet Kit Number be entered correctly. If you encounter errors, contact
dxsupport@
nanostring.com
for assistance.
6. Set E-mail Recipients
– If desired, choose e-mail recipients by selecting users from the contact list on the left side and clicking the
Add>>
button. Conversely, e-mail recipients can be removed by selecting the users from the list on the right side and clicking the
<<Remove
button (
FIGURE 4.21
). Press the
Ctrl
key (or the
Command
key if using an Apple computer) on the keyboard while selecting
multiple addresses to add or remove multiple recipients at once.
a. E-mail Status Updates to
– Users assigned here will receive e-mail updates anytime the Run Set status has changed.
b. E-mail Report Notifications to
– Users assigned here will receive an e-mail when the sample reports for the Run Set are
available for download. E-mails will include a link to log in and download the appropriate nCounter reports.
NOTE:
A user must have proper permissions set in their user profile in order to be able to receive e-mail report notifications.
Attempts to assign a user without appropriate access to receive report notifications will result in an error message. To change
these permissions, an administrator must edit the user’s profile and add the privilege.
