
Microplate
Reader
Operation
Manual
Chapter 5 Operation Guide
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21
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Fig 19
5.3 Spectral Analysis
See Fig 20, select target wells, click “Graph” button to enlarge the spectral curve, the
absorbance of each wavelength shown on the curve. Users can click “Back” button to the
graphic main interface.
Fig 20
Click “Spectral Analysis”, see Fig 21.
Calculation types includes:
Spectral Maximum: read the maximum value greater than the threshold value
within a set range.
Spectral Normalization
:
set the spectral range, take the maximum absorption peak
as 1, the remaining values will be converted into percentage based on this