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Microplate

 

Reader

 

Operation

 

Manual

Chapter 5 Operation Guide

─

14

─

Table 2

Endpoint

Kinetic

Spectrum

┗

Selected Parameters

┗

Selected Parameters

┗

Selected Parameters

┗

Wavelength

┗

Mode

┗

Fast

┗

Precision

┗

Wavelength

┗

λ1 (405)

┗

λ2 (450)

┗

λ3 (492)

┗

λ4 (630)

┗

Wavelength

┗

Mode

┗

Fast

┗

Precision

┗

Wavelength

┗

λ1 (405)

┗

λ2 (450)

┗

λ3 (492)

┗

λ4 (630)

┗

Wavelength

┗

Mode

┗

Fast

┗

Precision

┗

Wavelength

┗

Start Wavelength

┗

End Wavelength

┗

Step

┗

Kinetic

┗

Total Time

┗

Total Time

┗

Kinetic region

┗

No. of readings

┗

Number

┗

Kinetic region

┗

Shake

┗

Speed

┗

Low

┗

Medium

┗

High

┗

Type

┗

Continuous

┗

Pulsed

┗

Time

┗

Shake

┗

Speed

┗

Low

┗

Medium

┗

High

┗

Type

┗

Continuous

┗

Pulsed

┗

Time

┗

Shake

┗

Speed

┗

Low

┗

Medium

┗

High

┗

Type

┗

Continuous

┗

Pulsed

┗

Time

┗

Wait Time at start

┗

Wait Time at start

┗

Wait Time at start

┗

Area Selection

┗

Area Selection

┗

Area Selection

About

wavelength

option,

Endpoint

and

Kinetic

configured

with

four

wavelengths(defaults are 405nm, 450nm, 492nm and 630nm), users can click modify

wavelength by manual, but the value must be within the range 200nm~1000nm. Spectral

analysis can accept wavelength of any band, but also should be within 200nm~1000nm.

In addition, there is a button named “Log” on top right corner of the main display area,

the button is only available after the current protocol been executed.

The “Log” is mainly used for recording the finishing time of each step of the protocol.

Note: Steps can not be edited if a protocol been executed, users can save it as a new

one and then edit.

Summary of Contents for UT-200A

Page 1: ...Operation Manual UT 200A Microplate Reader PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION Hagavish st Israel 58817 Tel 972 3 5595252 Fax 972 3 5594529 mrc mrclab com MRC 1 22 ...

Page 2: ...Version modification Record Version No Date Modification Description V1 0 2019 12 16 Initial Release V2 0 2020 5 11 Revised performance parameters and add accessories included ...

Page 3: ...nstrument works and the operation guide please read carefully before operation and keep for future reference Opening check Please check the instruments as well as all accessories with packing list when you first open it If you find any wrong or missing please contact distributor or manufacturer ...

Page 4: ...t on the scheduled using life of the Instrument and the protection provided Indoor use only Warning Biological contamination All samples for test quality control calibration are regarded as infectious and any part contact with samples will also need to be treated as infectious Please wear gloves when operate this device Before using the device read the Manual carefully These units are designed for...

Page 5: ... socket when you pull out the plug and don t pull the electric line only The Instrument should be put in the place of low temperature less dust no water and no sun or strong lamp What s more the place should be good ventilation no corrosively gas or strong disturbing magnetic field far away from central heating camp stove and other hot resource Power off when you finish your work Pull off the conn...

Page 6: ...hapter 4 Installation 4 1 Opening check 4 2 Installation 4 3 Installation steps 4 Chapter 5 Operation Guide 6 1 Instrument self check 6 2 System settings 7 3 Protocol Management 8 4 Read a Microplate 10 5 Result processing 16 6 Report exporting 23 7 Power off 23 Chapter 6 Maintenance storage transportation 24 1 Maintenance 24 2 Storage and transportation 24 Chapter 7 Trouble shooting 25 Chapter 8 ...

Page 7: ... can be used alone and also connect with PC by ReaderIt II software for plenty of data analysis 4 96 well visual layout allows easy setting of blank sample positive negative quality control and multi value comparison 5 With dual optical system as well as reference optical channel which guarantee stable detection data 6 End point method kinetics and spectral scanning are available as well as plates...

Page 8: ...ty 0 2nm Read out range 0 0 4 0 OD Linearity 450nm R2 0 999 0 0 3 0 Accuracy 450nm 1 0 0 003A 0 2 0 2 0 2 0 2 5 Precision 450nm CV 0 5 Precision mode CV 1 0 Fast mode Stability 450nm 0 005A 0 0 2 0 2 2 0 2 5 Measurement speed 8 seconds at Fast mode 96 well plate 28 seconds at Precision mode 96 well plate Plate shaking Linear Incubation range RT 4 C to 45 C Temp uniformity 0 5 C 37 C Connections 1 ...

Page 9: ...al Chapter 3 Instrument Structure 3 Chapter 3 Instrument Structure Structure Rear cover Touch screen Roof cover External Button Plate holder Front door Extension module USB Port LAN Port Switch Power Socket B type USB Port Air outlet ...

Page 10: ... 2 Installation Working condition locate instrument on a flat dry and clean work table keep the front side with enough space for plate holder in and out also keeping 15cm space for back left and right side to enable put or connect wires Working environment a Clean air free from corrosion steam or smoke b Temperature should be within the range of 10 C 40 C c Relative humidity should be within the r...

Page 11: ...ace of the power adapter please connect it to the power according to the direction of the above picture 3 Switch I O button to I to turn on the instrument the front panel will cycle through a start up and self test screen Warning Don t connect instrument to power socket without ground wire ...

Page 12: ...on Guide 6 Chapter 5 Operation Guide 1 Instrument self check This chapter introduces default protocol operation beginning with self check after power on Refer to the picture below Fig 2 User login interface will appear after self checking see Fig 3 Fig 3 ...

Page 13: ...ed by Admin Default is 123456 or set when creating Only for their own files Guest Can not be deleted No password Note Please keep the Admin password or contact the manufacturer or your distributor when forgotten the password of Admin Home interface as below Fig 4 Fig 4 The Admin button on the lower left is for logout to login interface 2 System settings Users can can make system settings according...

Page 14: ...en for users 3 Click Home button on top left corner for the main interface 3 Protocol Management Click Absorbance to Fig 6 interface This interface mainly composed by six parts the navigation bar at the top the sidebar the main display area the optional bar switching bar type select area and the status bar at the lower right area Fig 6 The navigation bar The optional bar Switching bar The Sidebar ...

Page 15: ...t will pop up when a modified protocol not saved Save is for the current protocol saving which can be found in protocol list A shortcut for exporting raw data to U disk in format of csv Including sorting delete import export rename and save as etc Protocol list will be appear if clicking Protocols button see Fig 7 click blank area will close the protocol list interface Unfold state Protocol edit s...

Page 16: ... see Fig 7 unfold state Account Protocols of other accounts can be checked but this function is only available for Admin account When protocol list in edit state below operations are available Sorting protocols can be sorted according to Name Date and State Checkbox can be chosen for more than one for batch operation Delete delete selected protocols Export for exporting selected protocols to U dis...

Page 17: ...ration and unit of the first well the protocol will finish subsequent standard samples automatically according to the set operator and step size also users can modify by manual by clicking corresponding wells Eg If the set concentration is 125ng μL the operator is and the step size is 2 the concentration of the first well should be 125ng μL the second well 250ng μL and the third 500ng μL etc Sampl...

Page 18: ...g for example users can set all wells as unknown do absorbance test then layout of plate The test data and layout are separated users can modify or analyze the layout of historical data in real time Remark the absorbance value of the test can not be changed 4 2 Parameter setting After finishing the Elisa plate layout settings click Run on the side bar to Fig 10 Fig 10 ...

Page 19: ...function can be on or off Progress bar displays the running status of the current protocol Plate in out control the plate holder in and out also there is Physical button on the front of the enclosure Start Stop also Physical button on the front of the enclosure Shake this button is independent from the shaking function in settings and specially used for plate defoaming Meanwhile the optional bar t...

Page 20: ...Medium High Type Continuous Pulsed Time Wait Time at start Wait Time at start Wait Time at start Area Selection Area Selection Area Selection About wavelength option Endpoint and Kinetic configured with four wavelengths defaults are 405nm 450nm 492nm and 630nm users can click modify wavelength by manual but the value must be within the range 200nm 1000nm Spectral analysis can accept wavelength of ...

Page 21: ...ee Fig 11 Fig 11 Click button on screen or pressing Start button on the enclosure If the protocol has been implemented a hint will pop out for re naming the protocol input a new name click ok to run the protocol meanwhile the plate holder will move into the Reader for sample detection the screen will turn dark as Fig 12 and all buttons will unavailable except stop button or users can press Stop bu...

Page 22: ...modes Endpoint Kinetic and Spectrum 5 1 Endpoint result As Fig 13 shows data processing type of endpoint result includes Raw Data Blank Subtraction Basic Calculation Standard Curve Classification and Quality Control Raw data displays absorbance values of each well users can switch different wavelengths by pressing the button in the middle upper of the main interface Blank Subtraction blank absorba...

Page 23: ...p 1 button to switch to other standard curves Note The fitting type must be same when several groups of standard curves or users can not export them together only can export one by one Fig 14 If the fitting is not that good users can modify the fitting type from Parameters on the right of the interface or perform curve fitting after preprocessing the measured absorbance value and the input standar...

Page 24: ...e Qualitative analysis According to the negative and positive reference set in the layout interface samples can be qualitative analyzed as shown in Fig 15 input corresponding formula on the right side of the interface the instrument will mark negative or positive sample wells automatically positive is marked with low positive is with negative is without any mark Fig 15 Note Protocol layout interfa...

Page 25: ...ce must be set with Quality well otherwise this button will be not available 5 2 Kinetics As Fig 17 shows the main display area is not absorbance values but kinetic curves Fig 17 Choose the target well click Graph button can enlarge the kinetic curve see Fig 18 then click Back to graph main interface ...

Page 26: ...sis as the above Fig 18 shows For functions and algorithms of Raw Data Blank Subtraction and Basic Calculation please see Fig 5 1 Click Kinetic Analysis button the optional bar on the right side will change see Fig 19 At present it includes below calculations Average SD CV Integral Baseline Subtraction Select Single Reading Select Reading Range Maximum Rate Maximum Peak ...

Page 27: ...velength shown on the curve Users can click Back button to the graphic main interface Fig 20 Click Spectral Analysis see Fig 21 Calculation types includes Spectral Maximum read the maximum value greater than the threshold value within a set range Spectral Normalization set the spectral range take the maximum absorption peak as 1 the remaining values will be converted into percentage based on this ...

Page 28: ...gth Fig 21 Description of each button on the upper right corner of the main display area Print the current content of the main display area Export the current content of the main display area in picture format to U disk Choose the well to view the kinetic curve Graph button can enlarge the curve see Fig 16 Back is for graphic main interface Switch graphic display to data list display click Plate b...

Page 29: ...area four formats are available Xls Csv Pdf Txt Choose the content to export in Output Content on the right side will appear then click Output to export data to U disk Print due to too much of data print function is only for instrument basic information including instrument serial number software version etc 7 Power off Remove ELISA plate from the Reader then plate in the plate holder to the chamb...

Page 30: ...intenance list Content Day Wee k Year When needed Make sure the instrument power off correctly Keep the instrument away from dust Remove overflowing solution right away in case any damage then clean it by deionized distilled water If the surface been contaminated with a biohazard sterilize it by mild disinfectant Clean instrument enclosure regularly Clean the plate holder when necessary Verificati...

Page 31: ...facturer 7 E401 E403 E415 E425 E435 E445 when self checking Motor failure Please contact your distributor or manufacturer 8 E011 E056 when self checking Incubation failure Please contact your distributor or manufacturer 9 Test results are greatly deviated or all are zero Xenon lamp damaged Restart the instrument and try again if still same problem please contact your distributor or manufacturer 10...

Page 32: ...cessories 26 Chapter 8 Accessories No Item Type Unit Qty Remarks 1 Power cord PCS 1 2 Adapter 24V 160W PCS 1 3 Mouse Logitech PCS 1 4 Performance test statement PCS 1 5 Operation Manual PCS 1 6 Packing List PCS 1 7 U Disk PCS 1 8 Certification PCS 1 ...

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