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Molecular Devices SkanWasher 300, User Manual

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iv

SkanWasher 300 User Guide

Replacing Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Microplate Lift Adjustment (Tilt and Vertical). . . . . . . . 33

Chapter 7: 

Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

Troubleshooting Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Error Messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Vacuum pump Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . 42

Adjusting vacuum pump . . . . . . . . . . . . . . . . . . . . . . . . . 42
Discharge test for vacuum pump . . . . . . . . . . . . . . . . . . . 43
Compare pump with internal vacuum sensor  . . . . . . . . . 43

Application Tips: SkanWasher 300/ SkanStacker . . . . . . . . . 44

Appendix A: 

Serial Communication Option (RS-232) . . . . . . . . . . . . . . . A-1

Installation of Serial Communication option . . . . . . . . . . . . A-1
Parameters  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-2
Communication Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . A-3

Appendix B: 

Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-7

Physical. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-7
Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-7
Chemical. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . B-7

Appendix C: 

 Shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . C-6

Appendix D: 

 Decontamination Form . . . . . . . . . . . . . . . . . . . . . . . . . . . . D-8

Appendix E: 

Wash Program Sheets. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  E-9

Appendix F: 

 Warranty  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  F-15

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  1-1

Summary of Contents for SkanWasher 300

Page 1: ...SkanWasher 300 User Guide AN ALL INCLUSIVE AUTOMATIC MICROPLATE WASHER...

Page 2: ...ation MDC and is not to be used in any type of promotion or advertising without permission from MDC All other trademarks or registered trademarks are the property of their respective owners Disclaimer...

Page 3: ...tions 11 Pressure Vacuum Washer Parts 13 External Vacuum Source 13 Hydrophobic filter 13 Internal Pressure Source 13 Chapter 4 Software Configuration 14 Setup Function 15 Edit Program 15 Edit Options...

Page 4: ...cuum pump 43 Compare pump with internal vacuum sensor 43 Application Tips SkanWasher 300 SkanStacker 44 Appendix A Serial Communication Option RS 232 A 1 Installation of Serial Communication option A...

Page 5: ...7 Control Panel LCD and Touch Keys 3 10 Figure 8 Rear of the SkanWasher 300 3 11 Figure 9 Aspirate probe height position 4 17 Figure 10 Dispense probe height position 4 17 Figure 11 Wash Head Inlet C...

Page 6: ...vi SkanWasher 300 User Guide Table 1 Wash Step Selections 4 18 Table 2 Troubleshooting Chart 7 39 Tables...

Page 7: ...assay The SkanWasher 300 is designed to wash 96 well microplates The process of setting up wash parameters is flexible and allows you to modify or choose the following settings Aspirate probe height a...

Page 8: ...be listed for care and maintenance of instrument Chapter 7 Troubleshooting tips error messages and advanced troubleshoot ing will be outlined Appendix This section will include instrument specificati...

Page 9: ...lt page 19 for procedure Serial Number The serial number of the unit can be found on the left side of the instrument Package Content The SkanWasher 300 system includes the following SkanWasher 300 Ins...

Page 10: ......

Page 11: ...ll white mark on the bottom of the casing Verify correct fuses are in place change if indicated z 230 VAC 1A fuse z 115 VAC 2A fuse Reinstall switch Step 3 Connect the power cord Insert the power cord...

Page 12: ...ect vacuum tubing from Vacuum quick connect on waste reservoir to tubing from vacuum pump Hydrophobic filter should be connected to tubing from vacuum pump with arrow on filter facing pump Figure 1 Wa...

Page 13: ...the appropriate plate type round or flat bottom wells in use by selecting the correct printed pattern on the side of the microplate holder See Figure 3 Push the plate holder onto the two rods on the...

Page 14: ...Installation 7 SkanWasher 300 User Guide Figure 3 Microplate holder flat or round bottom plates Figure 4 Microplate holder and lift...

Page 15: ...portation screws on top of the washhead see Figure 5 Replace screws with two 2 PCV grip screws included with packaging using the Allen key from tool kit Step 8 Set up vacuum pump and adjust if necessa...

Page 16: ......

Page 17: ...wash head is removable using the two adjustment knobs to allow access to its internal channels for cleaning and maintenance Microplate Lift The microplate lift ensures that the microplate is position...

Page 18: ...lower when the instrument is powered on Control Panel The control panel located on the front of the SkanWasher 300 contains eight touch keys three LEDs and an LCD display all of which are used to pro...

Page 19: ...Press the Stop key to interrupt current operation and the LCD will display a stop message Press the Stop key a second time to return to the main menu The microplate lift will move down to home posi ti...

Page 20: ...nd rinse reservoir bottle Main power Located on the back panel of the unit This contains the main power receptacle which includes the power switch and fuses 1 amp for 230 Vac 2 amp for 115 Vac The sel...

Page 21: ...to protect the pump by preventing liquid from entering into the pump system A filter with a pore size of 0 2 m is recom mended The pump protection membrane filter with continued use will reduce flow c...

Page 22: ......

Page 23: ...ation can be varied The amount of fluid kept in the well can be adjusted Dispense probe height position in the well during washing can be set Up to 16 wash sequence steps can be selected Each step can...

Page 24: ...key depressed When pressing the Setup key the Setup Main menu will display two options Edit Program A wash program can be edited or created using this selection Edit Options Instrument parameters can...

Page 25: ...st the inlet wash liquid flow To disable the pressure pump select 0 0 bar For normal wash select 0 25 bar approximately 4 psi Volume Adjust Adjustment can be made to fine tune the dispensed vol ume us...

Page 26: ...in lift and press Enter to raise microplate to probes Use arrow keys to set at correct height see Figure 10 Total adjustable distance is 6 mm Wash Program 1 16 Up to 16 wash steps can be added There...

Page 27: ...ume of wash from 100 900 l Liquid will be dis pensed from Inlet port Wash time Set wash time from 0 1 to 10 seconds Liquid will be dis pensed from Inlet port Aspirate Set aspiration time from 2 0 10 s...

Page 28: ...r On Off Read Sensor Sensor On Off The sensors can be enabled or disabled by selecting this option Step 1 Press Setup on the Control Panel to enter the Setup Main Menu Step 2 Press Down arrow key to s...

Page 29: ...ings IMPORTANT When sensors disabled all four safety features will be disengaged Air Pressure Value is actual air pressure on internal pressure source displayed in bar Changes can be made through soft...

Page 30: ......

Page 31: ...on the side of microplate holder to verify that the holder is in the correct plate configuration Press Rinse key to enter prime program Press Start z Prime program will prime both Rinse and Inlet chan...

Page 32: ...late holder Check pattern on the side of microplate holder to verify that the holder is in the correct plate configuration Step 7 Press Start key Step 8 Repeat Step 5 8 to wash additional plates Step...

Page 33: ...Press the Rinse key to enter the Rinse Prime mode Step 5 Press the Rinse key a second time to start the rinse program The rinse program will rinse for 5 seconds using fluid from the Rinse port and the...

Page 34: ...he instru ment Step 4 Verify waste tubing is connected to vacuum port and vacuum pump is on Step 5 Insert an empty microplate into the microplate holder Step 6 Reverse flush the wash head through Rins...

Page 35: ...waste tubing to vacuum port Reconnect liquid and pressure tubing to correct ports Step 10 Enable sensors z Go to Setup Main Menu select Edit Options choose Sensor On Off z Enable sensors Step 11 Instr...

Page 36: ...le with bottle filled with decontami nate solution Connect tubing from reservoir bottle to Rinse port and pressure tubing to pressure port Verify waste tubing is connected to vacuum port and vacuum pu...

Page 37: ...de Press the Rinse key a second time to start the rinse program The rinse program will rinse for 5 seconds and then soak the probe tips for 5 seconds This cycle is repeated 3 times Step 9 Repeat Rinse...

Page 38: ...r Rinse Inlet Channels Clogged Probes Probes have the potential of being clogged by small particles from the wash liquid crystallization or protein build up It is important to perform Daily and Weekly...

Page 39: ...h head Step 3 Disable sensors from Setup Edit Option Sensor On Off consult page 19 for instructions Step 4 Fill a reservoir bottle with distilled water Step 5 Connect tubing to instrument Connect rins...

Page 40: ...Maintenance 30 SkanWasher 300 User Guide Figure 11 Wash Head Inlet Channel Seal Block...

Page 41: ...ash head block to expose the outlet channels Step 2 Observe for particles in outlet channel Step 3 If particles not removed and one probe can be identified to be clogged replace probe Or If particles...

Page 42: ...h pliers Remove old glue particles from wash head Step 3 Insert new probe burr diameter 1 10 mm Apply Loctite Activator no 7649 on the mounting area on the probe Push probe in until it touches the was...

Page 43: ...e column angle adjustment To be performed when columns 1 12 are not aligned to the aspiration probes Step 1 Set aspiration probe height through the program configuration in the software Step 2 Loosen...

Page 44: ...are aligned to rows A through H If nec essary re adjust Aspiration Probe horizontal adjustment To be performed when the aspiration probes do not enter the wells or are posi tioned incorrectly at the...

Page 45: ...Maintenance SkanWasher 300 User Guide 35 Figure 14 Adjustment Procedure Lift Version A 1 4 2 3 B A...

Page 46: ...to columns 1 through 12 If necessary re adjust Aspiration Probe row angle adjustment To be performed when rows A H are not aligned to the aspiration probes see Figure 16 Step 1 Set aspiration probe h...

Page 47: ...rain wash head Turn instrument off Unscrew the wash head locking screws 2 Lift the wash head up slowly Disconnect the air pressure supply tube and the ribbon cable from the wash head Step 2 Locate the...

Page 48: ...Maintenance 38 SkanWasher 300 User Guide Figure 16 Row Angle Adjustment Lift Version B...

Page 49: ...dies No power to instru ment instrument will not turn on z Check that power cord is plugged in z Check power supply z Check voltage selection switch z Check fuses Consult Chapter 2 Installation Air in...

Page 50: ...instrument memory Battery on main board may be low battery life is from three to five years Test the battery strength to see if it needs to be replaced Strength should be 3 68 volts 5 Replace battery...

Page 51: ...r 1 bar Verify vacuum pump is switched on Verify tubing connected properly Verify caps on reservoir bottles and waste collection vessel are tight E 02 Air Pressure Pressure High Low Verify Setup The S...

Page 52: ...washer Step 5 Test the vacuum system performance Record the reading from the manometer at 30 seconds 60 seconds 120 seconds and at 3 4 min utes The expected performance 10 is as follows With a start...

Page 53: ...ve reading on vacuum pump manometer Reading should drop to 950 1000 mbar in less than 10 seconds Step 4 If test failed check for obstruction Compare pump with internal vacuum sensor Step 1 Go to Setup...

Page 54: ...rom pump gauge Vacuum pressure determines the rate of aspiration Increasing the vacuum pressure value decreases the vacuum To convert mm Hg to mbar pressure consult conversion table at web site http w...

Page 55: ...e the aspirate probe must be close to the bottom of the well Check Dispense position in Edit Program from the Setup Menu in the software Re set height of dispense probes Residual volume too high or lo...

Page 56: ......

Page 57: ...t computer connector is a female 9 pin D SUB that can be connected directly to a serial port on a PC Installation of Serial Communication option Step 1 Exchange 3 pin connector for the 5 pin connector...

Page 58: ...ed with kit to connect to waste and RS 232 cable to connect to host computer Figure 17 SW300 Main PCB Connectors Parameters Baud rate 9 600 baud Data bits 8 Parity None Stop bits 1 The communication p...

Page 59: ...sensor check is enabled vacuum level air pressure waste level and microplate sensor will be verified prior to initiating the wash Start command accepted Reply G Not accepted due to sensor alarm Reply...

Page 60: ...program that was not defined The SkanWasher 300 will accept a select program command only if the wash program number between 1 and 8 has been defined 1 31h 2 32h 8 38h The wash program now selected 3F...

Page 61: ...whether or not a microplate is present will be verified prior to initiating the wash Start command accepted Reply G Not accepted due to sensor alarm Reply E When wash sequence finished Reply S 53h St...

Page 62: ......

Page 63: ...lectronic regulation Microplate lift Step motor driven Performance Aspirate 5 l average residual volume well Dispense z Accuracy 4 CV at 350 l z Volume 100 350 l Internal volume Wash head prime volume...

Page 64: ......

Page 65: ...ls are used use appropriate decontami nate solution for each material Step 2 Perform Empty Head procedure to empty dispense head of all fluid Step 3 Clean exterior surface of instrument with alcohol f...

Page 66: ......

Page 67: ...econtamination ______________________________________ _____________________________________________________________ _____________________________________________________________ ______________________...

Page 68: ......

Page 69: ...ak Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse 1 5 sec...

Page 70: ...pense Soak Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 250 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse...

Page 71: ...____ l 3 Wash Step Aspirate Wash Dispense Soak Rinse 2 0 sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec 200 l 5 Wash Step Aspirate Wash Dispense Soak Rinse 5 0 sec ____ l 6 Wash S...

Page 72: ...3 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 4 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 5 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 6 Wash Ste...

Page 73: ...ispense Soak Rinse _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soa...

Page 74: ...ispense Soak Rinse _____ sec ____ l 6 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 7 Wash Step Aspirate Wash Dispense Soak Rinse _____ sec ____ l 8 Wash Step Aspirate Wash Dispense Soa...

Page 75: ...esent proof of purchase documentation which includes the date of purchase and Molecular Devices Corporation must have the Warranty Registration form completed signed and returned by you within ten 10...

Page 76: ...of the bargain and does not constitute a warranty that the products shall conform to that description Any sample or model used in connection with this agree ment is for illustrative purposes only is n...

Page 77: ...amination 6 23 6 26 C 6 D 8 Dispense probes 4 14 4 17 6 32 Dispense test 6 28 Dispense volume 4 18 E Edit Options 4 15 4 19 Edit Program 4 15 ELISA setting 7 44 Empty Wash Head Procedure 6 24 EPROM A...

Page 78: ...1 3 Save 4 19 Sensor 4 19 Serial communication A 1 Serial number 1 3 Setup function 4 15 Setup key 3 10 3 11 Shipment C 6 Soak 4 18 Sodium hydroxide 6 26 Software 4 14 Specifications B 7 Stop key 3 11...

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