Molecular Devices SkanWasher 300, User Manual

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Maintenance
28 SkanWasher 300 User Guide
Advanced Maintenance
Dispense test
Step 1 Set up program for dispense test using the following parameters:
z
Aspirate 2 sec
z
Wash 350 µl
z
Aspirate 2 sec
z
Dispense 350 µl
z
END
Step 2 Run program and inspect dispense levels, note any low volumes.
Step 3 Repeat 3 times.
Step 4 Inspect each well and note if any wells have lower volumes. If low
volume consistent with all three dispenses, well must be cleared.
Follow procedure for
Clogged Probes . If inconsistent and lower vol-
ume has occurred on any of the three dispenses, perform an Empty
Head Procedure for a reverse flush of wash head.
Clogged Probes or Rinse Inlet Channels
Clogged Probes
Probes have the potential of being clogged by small particles from the wash
liquid, crystallization, or protein build-up. It is important to perform Daily and
Weekly maintenance to keep probes free of debris.
Step 1 Perform Daily Maintenance to rinse system.
Step 2 Perform Weekly Maintenance to clean probes.
Step 3 Clear particles from probes.
‹
Identify which probe is clogged.
‹
Use a probe wire to clear the probe.
Step 4 Replace Rinse reservoir bottle with reservoir bottle filled with dis-
tilled water.
Step 5 Verify waste tubing is connected to vacuum port and pump is on.
Step 6 Perform a forward flush to remove the particles.
‹
Insert a clean microplate into the microplate holder.
‹
Press the
[Rinse]
key to enter the Rinse/Prime mode.
‹
Press the
[Start]
key a second time to start the prime program.
‹
Repeat 3 times.