Molecular Devices SkanWasher 300, User Manual

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Troubleshooting
42 SkanWasher 300 User Guide
Vacuum pump Troubleshooting
Adjusting vacuum pump
Step 1 Start pump with only the vacuum line inserted to pump.
‹
If hydrophobic filter installed, make sure the ventilation hole is
covered.
‹
Turn manometer clockwise to close the regulator.
‹
Start the pump. Air should be flowing freely and the manometer
should read 950-1000 mbar.
Step 2 Close vacuum tubing by putting your finger over the opening.
Vacuum should reach a level of 50-100 mbar.
Step 3 Open manometer regulator screw about half a turn. The peak
vacuum will be reduced a few millibars, but this prevents condensa-
tion in the pump.
Step 4 Connect the empty 15-L waste collection vessel to vacuum pump
and washer.
Step 5 Test the vacuum system performance. Record the reading from the
manometer at 30 seconds, 60 seconds, 120 seconds, and at 3-4 min-
utes. The expected performance (+/- 10%) is as follows:
‹
With a start value of 1000 mbar, reading after 30 seconds should
be 500 mbar.
‹
With a start value of 1000 mbar, reading after 60 seconds should
be 300 mbar.
‹
With a start value of 1000 mbar, reading after 120 seconds should
be 200 mbar.
‹
With a start value of 1000 mbar, reading after a few minutes
should be 100 mbar.
Step 6 Evaluate system if performance not met. Rule out leaks in the sys-
tem and/or build up of condensated particles in filter. Repeat test if
indicated.
E-04 Waste Full
Waste Reservoir
¾¾ Empty
Waste
The
[Start]
key has
been pressed for wash
or rinse/prime, but the
waste reservoir is full.
Empty the waste reservoir
to continue.
Press the
[Stop]
key to
return from the Error posi-
tion.
Error Cause Things to Try