3
Table of Contents
Safety Information ----------------------------------------------------------------------------------------
5
General Guidelines --------------------------------------------------------------------------------------
7
Environment Conditions --------------------------------------------------------------------------------
8
Chapter 1
– Introduction
----------------------------------------------------------------------
9
1.1 Product Overview --------------------------------------------------------------------------------
9
1.2 Key Features --------------------------------------------------------------------------------------
10
1.3 Product Contents ---------------------------------------------------------------------------------
11
1.4 Product Specifications --------------------------------------------------------------------------
12
1.5 Product Description ------------------------------------------------------------------------------
13
Chapter 2
– Setting up
--------------------------------------------------------------------------
15
2.1 Installation ------------------------------------------------------------------------------------------ 15
2.2 Start-Up Screen ----------------------------------------------------------------------------------- 17
2.3 Settings ---------------------------------------------------------------------------------------------
18
2.4 Calibrating the Touchscreen (Calibrate Touchscreen) ----------------------------------
-
20
2.5 Changing Options for Cell Counting (Counting Options) -------------------------------
22
2.6 Setting the Date and Time ---------------------------------------------------------------------
25
2.7 Calibrating LUNA-
II™ (Calibrate) ------------------------------------------------------------
26
2.8 Updating the Software (Update Software) -------------------------------------------------
30
Chapter 3
– Protocol Setting
----------------------------------------------------------------
31
3.1 Parameters in the Protocol --------------------------------------------------------------------- 31
3.2 Setting the Protocols ----------------------------------------------------------------------------
33
3.3 Selection of Protocol ----------------------------------------------------------------------------- 37
Chapter 4
– Counting Cells
------------------------------------------------------------------
39
4.1 Preparing the Instrument -----------------------------------------------------------------------
39
4.2 Sample Preparation ------------------------------------------------------------------------------ 40
4.3 Loading Samples into the Luna
™ Cell Counting Slide ---------------------------------
-
40
4.4 Counting Cells ------------------------------------------------------------------------------------- 41
4.5 After Counting: Image View -------------------------------------------------------------------- 44
4.6 After Counting: Histogram & Gating ---------------------------------------------------------
46
4.7 After Counting: Saving and Printing the Results ------------------------------------------ 49