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Following screen will be displayed with increasing red bar over the time.
The Calibration Step 1 will take a few seconds. Do not turn off the instrument.
During the process, prepare diluted trypan blue solution by mixing 0.4% trypan blue stain with equal
volume of either distilled water, phosphate buffered saline (PBS), or plain medium.
Put 10
l of diluted trypan blue solution into the chamber of new cell counting slide (see Section 4.3).
After completion of the Calibration Step 1, following screen will be displayed.
Now, place the cell counting slide containing diluted trypan blue solution into the counting slide port.
Be sure the chamber containing the trypan blue solution to be placed inside the instrument. Do not
turn the cell counting slide upside-down.
