Leica CM1510 – Cryostat
17
8.1.1 Quick-freeze shelf with cover
The cryochamber has a quick-freeze shelf
(Fig. 17)
for
up to 10 specimen discs.
The supplied cover prevents excessive frost formation
on the quick-freeze shelf as the temperature of the shelf
is always lower than the cryochamber temperature.
The cover should only be removed when the quick-
freeze shelf is used for specimen freezing and replaced
when not used to minimize frost formation, especially
during defrosting.
Specimen freezing
•
Cut the specimen to size.
The specimen should be no greater than 2 cm x 2
cm x 2 cm.
•
Use a specimen disc at room temperature.
•
Apply enough cryocompound to the specimen disc.
•
Place the specimen on the disc and orient.
•
Remove the cover from the quick-freeze shelf.
•
Place the specimen disc with the specimen in one
of the holes of the quick-freeze shelf and freeze the
specimen.
Specimen freezing can be accelerated by ad-
ditionally using a mobile or stationary heat ex-
tractor, which are available as optional ac-
cessories (see Chapter 12. ‘Optional acces-
sories‘).
•
Once the specimen is frozen, insert the specimen
disc in the specimen head
(see Fig. 18.2)
and start
sectioning.
8.
Daily operation
8.1
Specimen freezing
Fig. 17
Summary of Contents for CM1510
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